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Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. 31P-NMR Spectroscopy
O. Dlouhý, U. Javornik, O. Zsiros, P. Šket, V. Karlický, V. Špunda, J. Plavec, G. Garab
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
19-13637S
Grantová Agentura České Republiky
K 128679
Hungarian Scientific Research Fund
CZ.02.1.01/0.0/0.0/16_019/0000797
SustES - Adaptation strategies for sustainable ecosystem services and food security under adverse environmental conditions
SGS02/PřF/2021
Ostravská Univerzita v Ostravě
07359/2019/RRC
Silesian Region
NLK
Directory of Open Access Journals
od 2012
Free Medical Journals
od 2012
PubMed Central
od 2012
Europe PubMed Central
od 2012
ProQuest Central
od 2012-03-01
Open Access Digital Library
od 2012-01-01
Open Access Digital Library
od 2012-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2012
PubMed
34572003
DOI
10.3390/cells10092354
Knihovny.cz E-zdroje
- MeSH
- chloroplasty chemie MeSH
- galaktolipidy chemie MeSH
- magnetická rezonanční spektroskopie metody MeSH
- membránové lipidy chemie MeSH
- teplota MeSH
- tylakoidy chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been documented that fully functional thylakoid membranes, in addition to the bilayer, contain an inverted hexagonal (HII) phase and two isotropic phases. To shed light on the origin of these non-lamellar phases, we performed 31P-NMR spectroscopy experiments on sub-chloroplast particles of spinach: stacked, granum and unstacked, stroma thylakoid membranes. These membranes exhibited similar lipid polymorphism as the whole thylakoids. Saturation transfer experiments, applying saturating pulses at characteristic frequencies at 5 °C, provided evidence for distinct lipid phases-with component spectra very similar to those derived from mathematical deconvolution of the 31P-NMR spectra. Wheat-germ lipase treatment of samples selectively eliminated the phases exhibiting sharp isotropic peaks, suggesting easier accessibility of these lipids compared to the bilayer and the HII phases. Gradually increasing lipid exchanges were observed between the bilayer and the two isotropic phases upon gradually elevating the temperature from 5 to 35 °C, suggesting close connections between these lipid phases. Data concerning the identity and structural and functional roles of different lipid phases will be presented in the accompanying paper.
EN FIST Center of Excellence SI 1000 Ljubljana Slovenia
Faculty of Chemistry and Chemical Technology University of Ljubljana SI 1000 Ljubljana Slovenia
Slovenian NMR Center National Institute of Chemistry SI 1000 Ljubljana Slovenia
Citace poskytuje Crossref.org
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