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Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing
C. Belser, FC. Baurens, B. Noel, G. Martin, C. Cruaud, B. Istace, N. Yahiaoui, K. Labadie, E. Hřibová, J. Doležel, A. Lemainque, P. Wincker, A. D'Hont, JM. Aury
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
ANR-10-LABX-0001-01
Agence Nationale de la Recherche (French National Research Agency)
ANR-10-INBS-09-08
Agence Nationale de la Recherche (French National Research Agency)
Nature Open Access od 2018-12-01
PubMed Central od 2018
Europe PubMed Central od 2018
ProQuest Central od 2018-01-01
ROAD: Directory of Open Access Scholarly Resources od 2018
Odkazy
PubMed
34493830
DOI
10.1038/s42003-021-02559-3
Knihovny.cz E-zdroje
- MeSH
- banánovník genetika MeSH
- chromozomy rostlin genetika MeSH
- genom rostlinný * MeSH
- nanoporové sekvenování MeSH
- nanopóry MeSH
- telomery * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Long-read technologies hold the promise to obtain more complete genome assemblies and to make them easier. Coupled with long-range technologies, they can reveal the architecture of complex regions, like centromeres or rDNA clusters. These technologies also make it possible to know the complete organization of chromosomes, which remained complicated before even when using genetic maps. However, generating a gapless and telomere-to-telomere assembly is still not trivial, and requires a combination of several technologies and the choice of suitable software. Here, we report a chromosome-scale assembly of a banana genome (Musa acuminata) generated using Oxford Nanopore long-reads. We generated a genome coverage of 177X from a single PromethION flowcell with near 17X with reads longer than 75 kbp. From the 11 chromosomes, 5 were entirely reconstructed in a single contig from telomere to telomere, revealing for the first time the content of complex regions like centromeres or clusters of paralogous genes.
CIRAD UMR AGAP Institut Montpellier France
Commissariat à l'Energie Atomique Institut François Jacob Genoscope Evry France
UMR AGAP Institut Univ Montpellier CIRAD INRAE Institut Agro Montpellier France
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