• Je něco špatně v tomto záznamu ?

Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

JC. Brandmeier, K. Raiko, Z. Farka, R. Peltomaa, MJ. Mickert, A. Hlaváček, P. Skládal, T. Soukka, HH. Gorris

. 2021 ; 10 (18) : e2100506. [pub] 20210715

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22003778

Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL-1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22003778
003      
CZ-PrNML
005      
20220127145856.0
007      
ta
008      
220113s2021 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1002/adhm.202100506 $2 doi
035    __
$a (PubMed)34263562
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Brandmeier, Julian C $u Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, 93053, Germany
245    10
$a Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin / $c JC. Brandmeier, K. Raiko, Z. Farka, R. Peltomaa, MJ. Mickert, A. Hlaváček, P. Skládal, T. Soukka, HH. Gorris
520    9_
$a Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL-1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.
650    _2
$a lidé $7 D006801
650    _2
$a imunoanalýza $7 D007118
650    12
$a nanočástice $7 D053758
650    _2
$a velikost částic $7 D010316
650    _2
$a fotony $7 D017785
650    _2
$a troponin $7 D014336
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Raiko, Kirsti $u Department of Life Technologies/Biotechnology, University of Turku, Kiinamyllynkatu 10, Turku, 20520, Finland
700    1_
$a Farka, Zdeněk $u Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, 93053, Germany $u Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, Brno, 625 00, Czech Republic
700    1_
$a Peltomaa, Riikka $u Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, 93053, Germany $u Department of Life Technologies/Biotechnology, University of Turku, Kiinamyllynkatu 10, Turku, 20520, Finland
700    1_
$a Mickert, Matthias J $u Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, 93053, Germany
700    1_
$a Hlaváček, Antonín $u Institute of Analytical Chemistry of the Czech Academy of Sciences, v. v. i., Brno, 602 00, Czech Republic
700    1_
$a Skládal, Petr $u Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, Brno, 625 00, Czech Republic
700    1_
$a Soukka, Tero $u Department of Life Technologies/Biotechnology, University of Turku, Kiinamyllynkatu 10, Turku, 20520, Finland
700    1_
$a Gorris, Hans H $u Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, 93053, Germany
773    0_
$w MED00189489 $t Advanced healthcare materials $x 2192-2659 $g Roč. 10, č. 18 (2021), s. e2100506
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34263562 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220113 $b ABA008
991    __
$a 20220127145852 $b ABA008
999    __
$a ok $b bmc $g 1751284 $s 1154927
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 10 $c 18 $d e2100506 $e 20210715 $i 2192-2659 $m Advanced healthcare materials $n Adv Healthc Mater $x MED00189489
LZP    __
$a Pubmed-20220113

Najít záznam

Citační ukazatele

Možnosti archivace