• Je něco špatně v tomto záznamu ?

Experimental transmission of Leishmania (Mundinia) parasites by biting midges (Diptera: Ceratopogonidae)

T. Becvar, B. Vojtkova, P. Siriyasatien, J. Votypka, D. Modry, P. Jahn, P. Bates, S. Carpenter, P. Volf, J. Sadlova

. 2021 ; 17 (6) : e1009654. [pub] 20210611

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22004217

Grantová podpora
BBS/E/I/00007039 Biotechnology and Biological Sciences Research Council - United Kingdom

Leishmania parasites, causative agents of leishmaniasis, are currently divided into four subgenera: Leishmania, Viannia, Sauroleishmania and Mundinia. The recently established subgenus Mundinia has a wide geographical distribution and contains five species, three of which have the potential to infect and cause disease in humans. While the other Leishmania subgenera are transmitted exclusively by phlebotomine sand flies (Diptera: Psychodidae), natural vectors of Mundinia remain uncertain. This study investigates the potential of sand flies and biting midges of the genus Culicoides (Diptera: Ceratopogonidae) to transmit Leishmania parasites of the subgenus Mundinia. Sand flies (Phlebotomus argentipes, P. duboscqi and Lutzomyia migonei) and Culicoides biting midges (Culicoides sonorensis) were exposed to five Mundinia species through a chicken skin membrane and dissected at specific time intervals post bloodmeal. Potentially infected insects were also allowed to feed on ear pinnae of anaesthetized BALB/c mice and the presence of Leishmania DNA was subsequently confirmed in the mice using polymerase chain reaction analyses. In C. sonorensis, all Mundinia species tested were able to establish infection at a high rate, successfully colonize the stomodeal valve and produce a higher proportion of metacyclic forms than in sand flies. Subsequently, three parasite species, L. martiniquensis, L. orientalis and L. sp. from Ghana, were transmitted to the host mouse ear by C. sonorensis bite. In contrast, transmission experiments entirely failed with P. argentipes, although colonisation of the stomodeal valve was observed for L. orientalis and L. martiniquensis and metacyclic forms of L. orientalis were recorded. This laboratory-based transmission of Mundinia species highlights that Culicoides are potential vectors of members of this ancestral subgenus of Leishmania and we suggest further studies in endemic areas to confirm their role in the lifecycles of neglected pathogens.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22004217
003      
CZ-PrNML
005      
20220127145430.0
007      
ta
008      
220113s2021 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.ppat.1009654 $2 doi
035    __
$a (PubMed)34115806
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Becvar, Tomas $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
245    10
$a Experimental transmission of Leishmania (Mundinia) parasites by biting midges (Diptera: Ceratopogonidae) / $c T. Becvar, B. Vojtkova, P. Siriyasatien, J. Votypka, D. Modry, P. Jahn, P. Bates, S. Carpenter, P. Volf, J. Sadlova
520    9_
$a Leishmania parasites, causative agents of leishmaniasis, are currently divided into four subgenera: Leishmania, Viannia, Sauroleishmania and Mundinia. The recently established subgenus Mundinia has a wide geographical distribution and contains five species, three of which have the potential to infect and cause disease in humans. While the other Leishmania subgenera are transmitted exclusively by phlebotomine sand flies (Diptera: Psychodidae), natural vectors of Mundinia remain uncertain. This study investigates the potential of sand flies and biting midges of the genus Culicoides (Diptera: Ceratopogonidae) to transmit Leishmania parasites of the subgenus Mundinia. Sand flies (Phlebotomus argentipes, P. duboscqi and Lutzomyia migonei) and Culicoides biting midges (Culicoides sonorensis) were exposed to five Mundinia species through a chicken skin membrane and dissected at specific time intervals post bloodmeal. Potentially infected insects were also allowed to feed on ear pinnae of anaesthetized BALB/c mice and the presence of Leishmania DNA was subsequently confirmed in the mice using polymerase chain reaction analyses. In C. sonorensis, all Mundinia species tested were able to establish infection at a high rate, successfully colonize the stomodeal valve and produce a higher proportion of metacyclic forms than in sand flies. Subsequently, three parasite species, L. martiniquensis, L. orientalis and L. sp. from Ghana, were transmitted to the host mouse ear by C. sonorensis bite. In contrast, transmission experiments entirely failed with P. argentipes, although colonisation of the stomodeal valve was observed for L. orientalis and L. martiniquensis and metacyclic forms of L. orientalis were recorded. This laboratory-based transmission of Mundinia species highlights that Culicoides are potential vectors of members of this ancestral subgenus of Leishmania and we suggest further studies in endemic areas to confirm their role in the lifecycles of neglected pathogens.
650    _2
$a zvířata $7 D000818
650    _2
$a Ceratopogonidae $x parazitologie $7 D002519
650    _2
$a hmyz - vektory $x parazitologie $7 D007303
650    12
$a Leishmania $7 D007891
650    _2
$a leishmanióza $x přenos $7 D007896
650    _2
$a myši $7 D051379
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Vojtkova, Barbora $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
700    1_
$a Siriyasatien, Padet $u Vector Biology and Vector Borne Disease Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
700    1_
$a Votypka, Jan $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
700    1_
$a Modry, David $u Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice, Czech Republic $u Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic $u Department of Veterinary Sciences/CINeZ, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
700    1_
$a Jahn, Petr $u Equine Clinic, University of Veterinary Sciences, Brno, Czech Republic
700    1_
$a Bates, Paul $u Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, United Kingdom
700    1_
$a Carpenter, Simon $u Entomology Group, The Pirbright Institute, Pirbright, Surrey, United Kingdom
700    1_
$a Volf, Petr $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
700    1_
$a Sadlova, Jovana $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
773    0_
$w MED00008922 $t PLoS pathogens $x 1553-7374 $g Roč. 17, č. 6 (2021), s. e1009654
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34115806 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220113 $b ABA008
991    __
$a 20220127145427 $b ABA008
999    __
$a ok $b bmc $g 1751619 $s 1155366
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 17 $c 6 $d e1009654 $e 20210611 $i 1553-7374 $m PLOS pathogens $n PLoS Pathog $x MED00008922
GRA    __
$a BBS/E/I/00007039 $p Biotechnology and Biological Sciences Research Council $2 United Kingdom
LZP    __
$a Pubmed-20220113

Najít záznam

Citační ukazatele

Možnosti archivace