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Variety of size and form of GRM2 bacterial microcompartment particles
EE. Cesle, A. Filimonenko, K. Tars, G. Kalnins
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1992 to 1 year ago
PubMed Central
from 1992 to 1 year ago
Europe PubMed Central
from 1992 to 1 year ago
Medline Complete (EBSCOhost)
from 2010-01-01 to 1 year ago
Wiley Free Content
from 1996 to 1 year ago
PubMed
33763934
DOI
10.1002/pro.4069
Knihovny.cz E-resources
- MeSH
- Bacterial Proteins chemistry metabolism MeSH
- Cryoelectron Microscopy MeSH
- Klebsiella pneumoniae chemistry metabolism ultrastructure MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Bacterial microcompartments (BMCs) are bacterial organelles involved in enzymatic processes, such as carbon fixation, choline, ethanolamine and propanediol degradation, and others. Formed of a semi-permeable protein shell and an enzymatic core, they can enhance enzyme performance and protect the cell from harmful intermediates. With the ability to encapsulate non-native enzymes, BMCs show high potential for applied use. For this goal, a detailed look into shell form variability is significant to predict shell adaptability. Here we present four novel 3D cryo-EM maps of recombinant Klebsiella pneumoniae GRM2 BMC shell particles with the resolution in range of 9 to 22 Å and nine novel 2D classes corresponding to discrete BMC shell forms. These structures reveal icosahedral, elongated, oblate, multi-layered and polyhedral traits of BMCs, indicating considerable variation in size and form as well as adaptability during shell formation processes.
CEITEC Central European Institute of Technology Masaryk University Brno Czech Republic
References provided by Crossref.org
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