Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds

PTN. Hoang, JM. Rouillard, J. Macas, I. Kubalová, V. Schubert, I. Schubert

. 2021 ; 130 (1) : 15-25. [pub] 20210114

Jazyk angličtina Země Rakousko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22004568
E-zdroje Online Plný text

NLK ProQuest Central od 1997-03-01 do Před 1 rokem
Health & Medicine (ProQuest) od 1997-03-01 do Před 1 rokem

Odkazy

PubMed 33443586
DOI 10.1007/s00412-020-00749-2
Knihovny.cz E-zdroje

Duckweeds represent a small, free-floating aquatic family (Lemnaceae) of the monocot order Alismatales with the fastest growth rate among flowering plants. They comprise five genera (Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia) varying in genome size and chromosome number. Spirodela polyrhiza had the first sequenced duckweed genome. Cytogenetic maps are available for both species of the genus Spirodela (S. polyrhiza and S. intermedia). However, elucidation of chromosome homeology and evolutionary chromosome rearrangements by cross-FISH using Spirodela BAC probes to species of other duckweed genera has not been successful so far. We investigated the potential of chromosome-specific oligo-FISH probes to address these topics. We designed oligo-FISH probes specific for one S. intermedia and one S. polyrhiza chromosome (Fig. 1a). Our results show that these oligo-probes cross-hybridize with the homeologous regions of the other congeneric species, but are not suitable to uncover chromosomal homeology across duckweeds genera. This is most likely due to too low sequence similarity between the investigated genera and/or too low probe density on the target genomes. Finally, we suggest genus-specific design of oligo-probes to elucidate chromosome evolution across duckweed genera.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22004568
003      
CZ-PrNML
005      
20220127145142.0
007      
ta
008      
220113s2021 au f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s00412-020-00749-2 $2 doi
035    __
$a (PubMed)33443586
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a au
100    1_
$a Hoang, Phuong T N $u Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Stadt Seeland, Germany $u Biology Department, Dalat University, District 8, Dalat City, Lamdong Province, Vietnam
245    10
$a Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds / $c PTN. Hoang, JM. Rouillard, J. Macas, I. Kubalová, V. Schubert, I. Schubert
520    9_
$a Duckweeds represent a small, free-floating aquatic family (Lemnaceae) of the monocot order Alismatales with the fastest growth rate among flowering plants. They comprise five genera (Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia) varying in genome size and chromosome number. Spirodela polyrhiza had the first sequenced duckweed genome. Cytogenetic maps are available for both species of the genus Spirodela (S. polyrhiza and S. intermedia). However, elucidation of chromosome homeology and evolutionary chromosome rearrangements by cross-FISH using Spirodela BAC probes to species of other duckweed genera has not been successful so far. We investigated the potential of chromosome-specific oligo-FISH probes to address these topics. We designed oligo-FISH probes specific for one S. intermedia and one S. polyrhiza chromosome (Fig. 1a). Our results show that these oligo-probes cross-hybridize with the homeologous regions of the other congeneric species, but are not suitable to uncover chromosomal homeology across duckweeds genera. This is most likely due to too low sequence similarity between the investigated genera and/or too low probe density on the target genomes. Finally, we suggest genus-specific design of oligo-probes to elucidate chromosome evolution across duckweed genera.
650    _2
$a Araceae $x klasifikace $x genetika $x růst a vývoj $7 D029064
650    _2
$a chromozomy rostlin $x genetika $7 D032461
650    12
$a molekulární evoluce $7 D019143
650    12
$a genom rostlinný $7 D018745
650    _2
$a hybridizace in situ fluorescenční $x metody $7 D017404
650    _2
$a karyotypizace $7 D007621
650    _2
$a oligonukleotidové sondy $x chemie $x genetika $7 D015345
650    _2
$a fylogeneze $7 D010802
650    _2
$a druhová specificita $7 D013045
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Rouillard, Jean-Marie $u Arbor Biosciences, Ann Arbor, MI, 48 102, USA $u Chemical Engineering Department, University of Michigan, Ann Arbor, MI, USA
700    1_
$a Macas, Jiří $u Biology Centre, Czech Academy of Sciences, Institute of Plant Molecular Biology, CZ 37005, České Budějovice, Czech Republic
700    1_
$a Kubalová, Ivona $u Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Stadt Seeland, Germany
700    1_
$a Schubert, Veit $u Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Stadt Seeland, Germany
700    1_
$a Schubert, Ingo $u Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Stadt Seeland, Germany. schubert@ipk-gatersleben.de
773    0_
$w MED00002160 $t Chromosoma $x 1432-0886 $g Roč. 130, č. 1 (2021), s. 15-25
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33443586 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220113 $b ABA008
991    __
$a 20220127145138 $b ABA008
999    __
$a ok $b bmc $g 1751884 $s 1155717
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 130 $c 1 $d 15-25 $e 20210114 $i 1432-0886 $m Chromosoma $n Chromosoma $x MED00002160
LZP    __
$a Pubmed-20220113

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace