• Je něco špatně v tomto záznamu ?

Magnetic nanoparticles of Ga-substituted ε-Fe2 O3 for biomedical applications: Magnetic properties, transverse relaxivity, and effects of silica-coated particles on cytoskeletal networks

K. Královec, R. Havelek, D. Koutová, P. Veverka, L. Kubíčková, P. Brázda, J. Kohout, V. Herynek, M. Vosmanská, O. Kaman

. 2020 ; 108 (7) : 1563-1578. [pub] 20200324

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22004886

Magnetic nanoparticles of ε-Fe1.76 Ga0.24 O3 with the volume-weighted mean size of 17 nm were prepared by thermal treatment of a mesoporous silica template impregnated with metal nitrates and were coated with silica shell of four different thicknesses in the range 6-24 nm. The bare particles exhibited higher magnetization than the undoped compound, 22.4 Am2 kg-1 at 300 K, and were characterized by blocked state with the coercivity of 1.2 T at 300 K, being thus the very opposite of superparamagnetic iron oxides. The relaxometric study of the silica-coated samples at 0.47 T revealed promising properties for MRI, specifically, transverse relaxivity of 89-168 s-1 mmol(f.u.)-1 L depending on the shell thickness was observed. We investigated the effects of the silica-coated nanoparticles on human A549 and MCF-7 cells. Cell viability, proliferation, cell cycle distribution, and the arrangement of actin cytoskeleton were assessed, as well as formation and maturation of focal adhesions. Our study revealed that high concentrations of silica-coated particles with larger shell thicknesses of 16-24 nm interfere with the actin cytoskeletal networks, inducing thus morphological changes. Consequently, the focal adhesion areas were significantly decreased, resulting in impaired cell adhesion.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22004886
003      
CZ-PrNML
005      
20220127144846.0
007      
ta
008      
220113s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1002/jbm.a.36926 $2 doi
035    __
$a (PubMed)32176405
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Královec, Karel $u Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic
245    10
$a Magnetic nanoparticles of Ga-substituted ε-Fe2 O3 for biomedical applications: Magnetic properties, transverse relaxivity, and effects of silica-coated particles on cytoskeletal networks / $c K. Královec, R. Havelek, D. Koutová, P. Veverka, L. Kubíčková, P. Brázda, J. Kohout, V. Herynek, M. Vosmanská, O. Kaman
520    9_
$a Magnetic nanoparticles of ε-Fe1.76 Ga0.24 O3 with the volume-weighted mean size of 17 nm were prepared by thermal treatment of a mesoporous silica template impregnated with metal nitrates and were coated with silica shell of four different thicknesses in the range 6-24 nm. The bare particles exhibited higher magnetization than the undoped compound, 22.4 Am2 kg-1 at 300 K, and were characterized by blocked state with the coercivity of 1.2 T at 300 K, being thus the very opposite of superparamagnetic iron oxides. The relaxometric study of the silica-coated samples at 0.47 T revealed promising properties for MRI, specifically, transverse relaxivity of 89-168 s-1 mmol(f.u.)-1 L depending on the shell thickness was observed. We investigated the effects of the silica-coated nanoparticles on human A549 and MCF-7 cells. Cell viability, proliferation, cell cycle distribution, and the arrangement of actin cytoskeleton were assessed, as well as formation and maturation of focal adhesions. Our study revealed that high concentrations of silica-coated particles with larger shell thicknesses of 16-24 nm interfere with the actin cytoskeletal networks, inducing thus morphological changes. Consequently, the focal adhesion areas were significantly decreased, resulting in impaired cell adhesion.
650    _2
$a buňky A549 $7 D000072283
650    _2
$a buněčný cyklus $x účinky léků $7 D002453
650    _2
$a viabilita buněk $x účinky léků $7 D002470
650    _2
$a cytoskelet $x účinky léků $x metabolismus $7 D003599
650    _2
$a galium $x chemie $x farmakologie $7 D005708
650    _2
$a lidé $7 D006801
650    _2
$a MFC-7 buňky $7 D061986
650    _2
$a magnetické nanočástice oxidů železa $x chemie $7 D000082662
650    _2
$a oxid křemičitý $x chemie $x farmakologie $7 D012822
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Havelek, Radim $u Faculty of Medicine in Hradec Králové, Charles University, Hradec Králové, Czech Republic
700    1_
$a Koutová, Darja $u Faculty of Medicine in Hradec Králové, Charles University, Hradec Králové, Czech Republic
700    1_
$a Veverka, Pavel $u Institute of Physics, Czech Academy of Sciences, Praha, Czech Republic
700    1_
$a Kubíčková, Lenka $u Institute of Physics, Czech Academy of Sciences, Praha, Czech Republic $u Faculty of Mathematics and Physics, Charles University, Praha, Czech Republic
700    1_
$a Brázda, Petr $u Institute of Physics, Czech Academy of Sciences, Praha, Czech Republic
700    1_
$a Kohout, Jaroslav $u Faculty of Mathematics and Physics, Charles University, Praha, Czech Republic
700    1_
$a Herynek, Vít $u First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Vosmanská, Magda $u Faculty of Chemical Engineering, University of Chemistry and Technology, Praha, Czech Republic
700    1_
$a Kaman, Ondřej $u Institute of Physics, Czech Academy of Sciences, Praha, Czech Republic
773    0_
$w MED00007498 $t Journal of biomedical materials research. Part A $x 1552-4965 $g Roč. 108, č. 7 (2020), s. 1563-1578
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32176405 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220113 $b ABA008
991    __
$a 20220127144842 $b ABA008
999    __
$a ok $b bmc $g 1752179 $s 1156035
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 108 $c 7 $d 1563-1578 $e 20200324 $i 1552-4965 $m Journal of biomedical materials research. Part A $n J Biomed Mater Res $x MED00007498
LZP    __
$a Pubmed-20220113

Najít záznam

Citační ukazatele

Možnosti archivace