Magnetic nanoparticles of ε-Fe1.76 Ga0.24 O3 with the volume-weighted mean size of 17 nm were prepared by thermal treatment of a mesoporous silica template impregnated with metal nitrates and were coated with silica shell of four different thicknesses in the range 6-24 nm. The bare particles exhibited higher magnetization than the undoped compound, 22.4 Am2 kg-1 at 300 K, and were characterized by blocked state with the coercivity of 1.2 T at 300 K, being thus the very opposite of superparamagnetic iron oxides. The relaxometric study of the silica-coated samples at 0.47 T revealed promising properties for MRI, specifically, transverse relaxivity of 89-168 s-1 mmol(f.u.)-1 L depending on the shell thickness was observed. We investigated the effects of the silica-coated nanoparticles on human A549 and MCF-7 cells. Cell viability, proliferation, cell cycle distribution, and the arrangement of actin cytoskeleton were assessed, as well as formation and maturation of focal adhesions. Our study revealed that high concentrations of silica-coated particles with larger shell thicknesses of 16-24 nm interfere with the actin cytoskeletal networks, inducing thus morphological changes. Consequently, the focal adhesion areas were significantly decreased, resulting in impaired cell adhesion.

1st Faculty of Medicine Charles University Prague Czech Republic

Faculty of Chemical Engineering University of Chemistry and Technology Praha Czech Republic

Faculty of Chemical Technology University of Pardubice Pardubice Czech Republic

Faculty of Mathematics and Physics Charles University Praha Czech Republic

Faculty of Medicine in Hradec Králové Charles University Hradec Králové Czech Republic

Institute of Physics Czech Academy of Sciences Praha Czech Republic

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$a Královec, Karel $u Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic

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$a Magnetic nanoparticles of Ga-substituted ε-Fe2 O3 for biomedical applications: Magnetic properties, transverse relaxivity, and effects of silica-coated particles on cytoskeletal networks / $c K. Královec, R. Havelek, D. Koutová, P. Veverka, L. Kubíčková, P. Brázda, J. Kohout, V. Herynek, M. Vosmanská, O. Kaman

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$a Magnetic nanoparticles of ε-Fe1.76 Ga0.24 O3 with the volume-weighted mean size of 17 nm were prepared by thermal treatment of a mesoporous silica template impregnated with metal nitrates and were coated with silica shell of four different thicknesses in the range 6-24 nm. The bare particles exhibited higher magnetization than the undoped compound, 22.4 Am2 kg-1 at 300 K, and were characterized by blocked state with the coercivity of 1.2 T at 300 K, being thus the very opposite of superparamagnetic iron oxides. The relaxometric study of the silica-coated samples at 0.47 T revealed promising properties for MRI, specifically, transverse relaxivity of 89-168 s-1 mmol(f.u.)-1 L depending on the shell thickness was observed. We investigated the effects of the silica-coated nanoparticles on human A549 and MCF-7 cells. Cell viability, proliferation, cell cycle distribution, and the arrangement of actin cytoskeleton were assessed, as well as formation and maturation of focal adhesions. Our study revealed that high concentrations of silica-coated particles with larger shell thicknesses of 16-24 nm interfere with the actin cytoskeletal networks, inducing thus morphological changes. Consequently, the focal adhesion areas were significantly decreased, resulting in impaired cell adhesion.

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$a Havelek, Radim $u Faculty of Medicine in Hradec Králové, Charles University, Hradec Králové, Czech Republic

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$a Koutová, Darja $u Faculty of Medicine in Hradec Králové, Charles University, Hradec Králové, Czech Republic

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$a Veverka, Pavel $u Institute of Physics, Czech Academy of Sciences, Praha, Czech Republic

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$a Kubíčková, Lenka $u Institute of Physics, Czech Academy of Sciences, Praha, Czech Republic $u Faculty of Mathematics and Physics, Charles University, Praha, Czech Republic

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$a Kohout, Jaroslav $u Faculty of Mathematics and Physics, Charles University, Praha, Czech Republic

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$a Herynek, Vít $u First Faculty of Medicine, Charles University, Prague, Czech Republic

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$a Vosmanská, Magda $u Faculty of Chemical Engineering, University of Chemistry and Technology, Praha, Czech Republic

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