Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Gene Profile of Adipose Tissue of Patients with Pheochromocytoma/Paraganglioma

J. Klímová, M. Mráz, H. Kratochvílová, Z. Lacinová, K. Novák, D. Michalský, J. Kvasnička, R. Holaj, D. Haluzíková, RP. Doležalová, M. Zítek, Z. Krátká, V. Todorovová, J. Widimský, M. Haluzík, T. Zelinka, O. Petrák

. 2022 ; 10 (3) : . [pub] 20220302

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc22009865

BACKGROUND: Brown adipose tissue (BAT) is a therapeutic target to combat obesity and related disorders. Pheochromocytoma and functional paraganglioma (PPGL) are associated with activated BAT due to catecholamine excess. Our aim was to evaluate BAT activity by gene profile and assess its relation to clinical characteristics and overproduced catecholamine. METHODS: mRNA expression of 15 genes in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) was measured via RT-PCR in 25 patients with PPGL and 14 controls undergoing cholecystectomy. RESULTS: We found in VAT of PPGL higher expression of UCP1 (p < 0.001), CEBPB, PPARGC1A (both p < 0.001), PRDM16 (p = 0.069) and DIO2 (p = 0.005). UCP1 expression correlated only with norepinephrine levels and its metabolite. UCP1 expression, among others, correlated negatively with BMI, age and positively with HDLc levels. Dominance of BAT or BeAT markers was not assessed in PPGL. In SAT of PPGL, we found higher expression of ADRB3, CIDEA (both p < 0.05), and PPARGC1A (p = 0.001), but not UCP1. CONCLUSION: We demonstrate signs of UCP1-dependent norepinephrine-induced thermogenesis connected with higher expression of DIO2, PPARGC1A, CEBPB and PRDM16 in retroperitoneal VAT of PPGL and its relations to circulating HDLc and triglycerides levels. However, no direct relationship with increased basal energy metabolism measured by calorimetry was found.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22009865
003      
CZ-PrNML
005      
20220425131608.0
007      
ta
008      
220420s2022 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/biomedicines10030586 $2 doi
035    __
$a (PubMed)35327387
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Klímová, Judita $u Center of Hypertension, 3rd Department of Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic $1 https://orcid.org/0000000200880277
245    10
$a Gene Profile of Adipose Tissue of Patients with Pheochromocytoma/Paraganglioma / $c J. Klímová, M. Mráz, H. Kratochvílová, Z. Lacinová, K. Novák, D. Michalský, J. Kvasnička, R. Holaj, D. Haluzíková, RP. Doležalová, M. Zítek, Z. Krátká, V. Todorovová, J. Widimský, M. Haluzík, T. Zelinka, O. Petrák
520    9_
$a BACKGROUND: Brown adipose tissue (BAT) is a therapeutic target to combat obesity and related disorders. Pheochromocytoma and functional paraganglioma (PPGL) are associated with activated BAT due to catecholamine excess. Our aim was to evaluate BAT activity by gene profile and assess its relation to clinical characteristics and overproduced catecholamine. METHODS: mRNA expression of 15 genes in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) was measured via RT-PCR in 25 patients with PPGL and 14 controls undergoing cholecystectomy. RESULTS: We found in VAT of PPGL higher expression of UCP1 (p &lt; 0.001), CEBPB, PPARGC1A (both p &lt; 0.001), PRDM16 (p = 0.069) and DIO2 (p = 0.005). UCP1 expression correlated only with norepinephrine levels and its metabolite. UCP1 expression, among others, correlated negatively with BMI, age and positively with HDLc levels. Dominance of BAT or BeAT markers was not assessed in PPGL. In SAT of PPGL, we found higher expression of ADRB3, CIDEA (both p &lt; 0.05), and PPARGC1A (p = 0.001), but not UCP1. CONCLUSION: We demonstrate signs of UCP1-dependent norepinephrine-induced thermogenesis connected with higher expression of DIO2, PPARGC1A, CEBPB and PRDM16 in retroperitoneal VAT of PPGL and its relations to circulating HDLc and triglycerides levels. However, no direct relationship with increased basal energy metabolism measured by calorimetry was found.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Mráz, Miloš $u Center for Experimental Medicine and Diabetes Center, Institute for Clinical and Experimental Medicine, 140 00 Prague, Czech Republic
700    1_
$a Kratochvílová, Helena $u Institute for Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Lacinová, Zdeňka $u Center for Experimental Medicine and Diabetes Center, Institute for Clinical and Experimental Medicine, 140 00 Prague, Czech Republic
700    1_
$a Novák, Květoslav $u Department of Urology, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Michalský, David $u First Department of Surgery, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Kvasnička, Jan $u Center of Hypertension, 3rd Department of Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Holaj, Robert $u Center of Hypertension, 3rd Department of Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Haluzíková, Denisa $u Institute of Sport Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Doležalová, Radka Petráková $u Institute of Sport Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Zítek, Matěj $u Center of Hypertension, 3rd Department of Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Krátká, Zuzana $u Center of Hypertension, 3rd Department of Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Todorovová, Veronika $u Laboratory of Endocrinology and Metabolism, 3rd Department of Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Widimský, Jiří $u Center of Hypertension, 3rd Department of Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Haluzík, Martin $u Center for Experimental Medicine and Diabetes Center, Institute for Clinical and Experimental Medicine, 140 00 Prague, Czech Republic
700    1_
$a Zelinka, Tomáš $u Center of Hypertension, 3rd Department of Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic
700    1_
$a Petrák, Ondřej $u Center of Hypertension, 3rd Department of Medicine, First Faculty of Medicine and General Faculty Hospital, Charles University, 128 00 Prague, Czech Republic $1 https://orcid.org/0000000189923562 $7 xx0104500
773    0_
$w MED00205373 $t Biomedicines $x 2227-9059 $g Roč. 10, č. 3 (2022)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35327387 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20220420 $b ABA008
991    __
$a 20220425131605 $b ABA008
999    __
$a ind $b bmc $g 1784417 $s 1161063
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 10 $c 3 $e 20220302 $i 2227-9059 $m Biomedicines $n Biomedicines $x MED00205373
LZP    __
$a Pubmed-20220420

Find record

Citation metrics

Archiving options