Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Circulating Tumor Cell Kinetics and Morphology from the Liquid Biopsy Predict Disease Progression in Patients with Metastatic Colorectal Cancer Following Resection

D. Kolenčík, S. Narayan, JA. Thiele, D. McKinley, AS. Gerdtsson, L. Welter, P. Hošek, P. Ostašov, O. Vyčítal, J. Brůha, O. Fiala, O. Šorejs, V. Liška, P. Pitule, P. Kuhn, SN. Shishido

. 2022 ; 14 (3) : . [pub] 20220127

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22010129

Grantová podpora
Progress Q39 Charles University
UNCE/MED/006 Charles University
D. Kolenčík Fulbright Association
USC16HSC FNIH Biomarkers Consortium
2015-06510 Swedish Research Council
L. Welter Alan Joseph Endowed Fellowship

The liquid biopsy has the potential to improve current clinical practice in oncology by providing real-time personalized information about a patient's disease status and response to treatment. In this study, we evaluated 161 peripheral blood (PB) samples that were collected around surgical resection from 47 metastatic colorectal cancer (mCRC) patients using the High-Definition Single Cell Assay (HDSCA) workflow. In conjunction with the standard circulating tumor cell (CTC) enumeration, cellular morphology and kinetics between time-points of collection were considered in the survival analysis. CTCs, CTC-Apoptotic, and CTC clusters were found to indicate poor survival with an increase in cell count from pre-resection to post-resection. This study demonstrates that CTC subcategorization based on morphological differences leads to nuanced results between the subtypes, emphasizing the heterogeneity within the CTC classification. Furthermore, we show that factoring in the time-point of each blood collection is critical, both for its static enumeration and for the change in cell populations between draws. By integrating morphology and time-based analysis alongside standard CTC enumeration, liquid biopsy platforms can provide greater insight into the pathophysiology of mCRC by highlighting the complexity of the disease across a patient's treatment.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22010129
003      
CZ-PrNML
005      
20220425131716.0
007      
ta
008      
220420s2022 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/cancers14030642 $2 doi
035    __
$a (PubMed)35158910
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Kolenčík, Drahomír $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic $1 https://orcid.org/0000000279245822
245    10
$a Circulating Tumor Cell Kinetics and Morphology from the Liquid Biopsy Predict Disease Progression in Patients with Metastatic Colorectal Cancer Following Resection / $c D. Kolenčík, S. Narayan, JA. Thiele, D. McKinley, AS. Gerdtsson, L. Welter, P. Hošek, P. Ostašov, O. Vyčítal, J. Brůha, O. Fiala, O. Šorejs, V. Liška, P. Pitule, P. Kuhn, SN. Shishido
520    9_
$a The liquid biopsy has the potential to improve current clinical practice in oncology by providing real-time personalized information about a patient's disease status and response to treatment. In this study, we evaluated 161 peripheral blood (PB) samples that were collected around surgical resection from 47 metastatic colorectal cancer (mCRC) patients using the High-Definition Single Cell Assay (HDSCA) workflow. In conjunction with the standard circulating tumor cell (CTC) enumeration, cellular morphology and kinetics between time-points of collection were considered in the survival analysis. CTCs, CTC-Apoptotic, and CTC clusters were found to indicate poor survival with an increase in cell count from pre-resection to post-resection. This study demonstrates that CTC subcategorization based on morphological differences leads to nuanced results between the subtypes, emphasizing the heterogeneity within the CTC classification. Furthermore, we show that factoring in the time-point of each blood collection is critical, both for its static enumeration and for the change in cell populations between draws. By integrating morphology and time-based analysis alongside standard CTC enumeration, liquid biopsy platforms can provide greater insight into the pathophysiology of mCRC by highlighting the complexity of the disease across a patient's treatment.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Narayan, Sachin $u Convergent Science Institute in Cancer, Michelson Center for Convergent Bioscience, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USA
700    1_
$a Thiele, Jana-Aletta $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic
700    1_
$a McKinley, Dillon $u Convergent Science Institute in Cancer, Michelson Center for Convergent Bioscience, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USA
700    1_
$a Gerdtsson, Anna Sandström $u Convergent Science Institute in Cancer, Michelson Center for Convergent Bioscience, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USA $1 https://orcid.org/0000000319320365
700    1_
$a Welter, Lisa $u Convergent Science Institute in Cancer, Michelson Center for Convergent Bioscience, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USA
700    1_
$a Hošek, Petr $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic $1 https://orcid.org/0000000293594770 $7 xx0224573
700    1_
$a Ostašov, Pavel $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic $1 https://orcid.org/0000000171686431
700    1_
$a Vyčítal, Ondřej $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic $u Department of Surgery, Faculty of Medicine and University Hospital in Pilsen, Charles University, 32300 Pilsen, Czech Republic
700    1_
$a Brůha, Jan $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic $u Department of Surgery, Faculty of Medicine and University Hospital in Pilsen, Charles University, 32300 Pilsen, Czech Republic
700    1_
$a Fiala, Ondřej $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic $u Department of Oncology and Radiotherapeutics, Faculty of Medicine and University Hospital in Pilsen, Charles University, 32300 Pilsen, Czech Republic $1 https://orcid.org/0000000240967385
700    1_
$a Šorejs, Ondřej $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic $u Department of Oncology and Radiotherapeutics, Faculty of Medicine and University Hospital in Pilsen, Charles University, 32300 Pilsen, Czech Republic
700    1_
$a Liška, Václav $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic $u Department of Surgery, Faculty of Medicine and University Hospital in Pilsen, Charles University, 32300 Pilsen, Czech Republic
700    1_
$a Pitule, Pavel $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic $1 https://orcid.org/0000000231502914 $7 xx0170503
700    1_
$a Kuhn, Peter $u Convergent Science Institute in Cancer, Michelson Center for Convergent Bioscience, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USA $1 https://orcid.org/0000000326294505
700    1_
$a Shishido, Stephanie N $u Convergent Science Institute in Cancer, Michelson Center for Convergent Bioscience, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USA
773    0_
$w MED00173178 $t Cancers $x 2072-6694 $g Roč. 14, č. 3 (2022)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35158910 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20220420 $b ABA008
991    __
$a 20220425131714 $b ABA008
999    __
$a ind $b bmc $g 1784495 $s 1161327
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 14 $c 3 $e 20220127 $i 2072-6694 $m Cancers $n Cancers $x MED00173178
GRA    __
$a Progress Q39 $p Charles University
GRA    __
$a UNCE/MED/006 $p Charles University
GRA    __
$a D. Kolenčík $p Fulbright Association
GRA    __
$a USC16HSC $p FNIH Biomarkers Consortium
GRA    __
$a 2015-06510 $p Swedish Research Council
GRA    __
$a L. Welter $p Alan Joseph Endowed Fellowship
LZP    __
$a Pubmed-20220420

Najít záznam

Citační ukazatele

Možnosti archivace