• Je něco špatně v tomto záznamu ?

Simultaneous determination of guanine and adenine in human saliva with graphite sparked screen-printed electrodes

AV. Papavasileiou, MG. Trachioti, J. Hrbac, MI. Prodromidis

. 2022 ; 239 (-) : 123119. [pub] 20211130

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22010922

Saliva represents one of the most useful biological samples for non-invasive testing of health status and diseases prognosis and therefore, the development of advanced sensors enabling the determination of biomarkers in unspiked human whole saliva is of immense importance. Herein, we report on the development of a screen-printed graphite sensor modified with carbon nanomaterials generated by spark discharge for the determination of guanine and adenine in unspiked human whole saliva. The designed sensor was developed with a "green", extremely simple, fast (16 s), fully automated "linear mode" sparking process implemented with a 2D positioning device. Carbon nanomaterial-modified surfaces exhibit outstanding electrocatalytic properties enabling the determination of guanine and adenine over the concentration range 5 - 1000 nM and 25 - 1000 nM, while achieving limits of detection (S/N 3) as low as 2 nM and 8 nM, respectively. The sensor was successfully applied to the determination of purine bases in unspiked human whole saliva following a simple assay protocol based on ultrafiltration that effectively alleviates biofouling issues. Recovery was 96-108%.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22010922
003      
CZ-PrNML
005      
20220506130208.0
007      
ta
008      
220425s2022 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.talanta.2021.123119 $2 doi
035    __
$a (PubMed)34864536
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Papavasileiou, Anastasios V $u Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece
245    10
$a Simultaneous determination of guanine and adenine in human saliva with graphite sparked screen-printed electrodes / $c AV. Papavasileiou, MG. Trachioti, J. Hrbac, MI. Prodromidis
520    9_
$a Saliva represents one of the most useful biological samples for non-invasive testing of health status and diseases prognosis and therefore, the development of advanced sensors enabling the determination of biomarkers in unspiked human whole saliva is of immense importance. Herein, we report on the development of a screen-printed graphite sensor modified with carbon nanomaterials generated by spark discharge for the determination of guanine and adenine in unspiked human whole saliva. The designed sensor was developed with a "green", extremely simple, fast (16 s), fully automated "linear mode" sparking process implemented with a 2D positioning device. Carbon nanomaterial-modified surfaces exhibit outstanding electrocatalytic properties enabling the determination of guanine and adenine over the concentration range 5 - 1000 nM and 25 - 1000 nM, while achieving limits of detection (S/N 3) as low as 2 nM and 8 nM, respectively. The sensor was successfully applied to the determination of purine bases in unspiked human whole saliva following a simple assay protocol based on ultrafiltration that effectively alleviates biofouling issues. Recovery was 96-108%.
650    _2
$a adenin $7 D000225
650    _2
$a elektrochemické techniky $7 D055664
650    _2
$a elektrody $7 D004566
650    12
$a grafit $7 D006108
650    _2
$a guanin $7 D006147
650    _2
$a lidé $7 D006801
650    _2
$a sliny $7 D012463
655    _2
$a časopisecké články $7 D016428
700    1_
$a Trachioti, Maria G $u Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece
700    1_
$a Hrbac, Jan $u Department of Chemistry, Masaryk University, 625 00, Brno, Czech Republic
700    1_
$a Prodromidis, Mamas I $u Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece; Institute of Materials Science and Computing, University Research Center of Ioannina (URCI), Ioannina, Greece. Electronic address: mprodrom@uoi.gr
773    0_
$w MED00004484 $t Talanta $x 1873-3573 $g Roč. 239, č. - (2022), s. 123119
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34864536 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220425 $b ABA008
991    __
$a 20220506130201 $b ABA008
999    __
$a ok $b bmc $g 1788845 $s 1162120
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 239 $c - $d 123119 $e 20211130 $i 1873-3573 $m Talanta $n Talanta $x MED00004484
LZP    __
$a Pubmed-20220425
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace