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Simultaneous determination of guanine and adenine in human saliva with graphite sparked screen-printed electrodes
AV. Papavasileiou, MG. Trachioti, J. Hrbac, MI. Prodromidis
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- adenin MeSH
- elektrochemické techniky MeSH
- elektrody MeSH
- grafit * MeSH
- guanin MeSH
- lidé MeSH
- sliny MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Saliva represents one of the most useful biological samples for non-invasive testing of health status and diseases prognosis and therefore, the development of advanced sensors enabling the determination of biomarkers in unspiked human whole saliva is of immense importance. Herein, we report on the development of a screen-printed graphite sensor modified with carbon nanomaterials generated by spark discharge for the determination of guanine and adenine in unspiked human whole saliva. The designed sensor was developed with a "green", extremely simple, fast (16 s), fully automated "linear mode" sparking process implemented with a 2D positioning device. Carbon nanomaterial-modified surfaces exhibit outstanding electrocatalytic properties enabling the determination of guanine and adenine over the concentration range 5 - 1000 nM and 25 - 1000 nM, while achieving limits of detection (S/N 3) as low as 2 nM and 8 nM, respectively. The sensor was successfully applied to the determination of purine bases in unspiked human whole saliva following a simple assay protocol based on ultrafiltration that effectively alleviates biofouling issues. Recovery was 96-108%.
Department of Chemistry Masaryk University 625 00 Brno Czech Republic
Department of Chemistry University of Ioannina Ioannina 45110 Greece
Institute of Materials Science and Computing University Research Center of Ioannina Ioannina Greece
Citace poskytuje Crossref.org
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- $a Prodromidis, Mamas I $u Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece; Institute of Materials Science and Computing, University Research Center of Ioannina (URCI), Ioannina, Greece. Electronic address: mprodrom@uoi.gr
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