Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Intestinal mycobiome associated with diagnosis of inflammatory bowel disease based on tissue biopsies

J. Cimická, J. Riegert, M. Kavková, K. Černá

. 2022 ; 60 (1) : . [pub] 2022Jan10

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22011361

Analysis of mycobiome from formalin-fixed, paraffin-embedded (FFPE) biopsies should preferentially detect only fungi which are actually present in the intestine wall, in contrast to stool samples, which are limited by the diet composition. Next generation sequencing provides the advantage of analyzing many species from a single sample. Consequently, canonical correspondence analysis divided fungal genera present in FFPE intestinal tissues into three well-defined experimental groups (negative controls - NC, Crohn's disease - CD, ulcerative colitis - UC). Simultaneously, the analysis showed that particular fungal genera are associated with these experimental groups and several fungal genera occurred in all experimental groups equally. Our results also showed a noticeable increase of Ascomycota proportion from NC, through CD to UC. Fungal genera Malassezia, Cladosporium and Toninia occurred in all experimental groups assuming that they are common components of the intestinal mycobiome. Other fungal genera found only in the NC experimental group were non-pathogenic and might bring some benefits. In contrast, CD and UC samples were characterized by an accumulation of genera with inhibitive effects on growth of other fungal genera and the presence of opportunistic pathogens. Furthermore, a decrease in the fungal genus Malassezia in inflammatory tissues was observed; Specifically, the UC experimental group showed a connection between the presence of Candida and seven time's lower amounts of Malassezia (compared to amounts found in NC). The CD experimental group was characterized by the simultaneous presence of Engyodontium album with Lecanicillium, and indicates a possible pathogenic effect of Ramularia in disease development.LAY SUMMARY: Mycobiome analysis of formalin-fixed and paraffin-embedded biopsies may highlight actual fungal genera composition in the intestinal wall. Interestingly, experimental groups of Crohn's disease and ulcerative colitis clearly differed by structure of their mycobiomes.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22011361
003      
CZ-PrNML
005      
20221102102019.0
007      
ta
008      
220425s2022 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/mmy/myab076 $2 doi
035    __
$a (PubMed)34850076
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Cimická, Jana $u Laboratory of Pain research, Institute of Physiology, Czech Academy of Sciences, 142 20 Prague, Czech Republic $u Department of Molecular Genetics, Bioptická Laboratoř s.r.o., 326 00 Pilsen, Czech Republic $1 https://orcid.org/0000000260028924
245    10
$a Intestinal mycobiome associated with diagnosis of inflammatory bowel disease based on tissue biopsies / $c J. Cimická, J. Riegert, M. Kavková, K. Černá
520    9_
$a Analysis of mycobiome from formalin-fixed, paraffin-embedded (FFPE) biopsies should preferentially detect only fungi which are actually present in the intestine wall, in contrast to stool samples, which are limited by the diet composition. Next generation sequencing provides the advantage of analyzing many species from a single sample. Consequently, canonical correspondence analysis divided fungal genera present in FFPE intestinal tissues into three well-defined experimental groups (negative controls - NC, Crohn's disease - CD, ulcerative colitis - UC). Simultaneously, the analysis showed that particular fungal genera are associated with these experimental groups and several fungal genera occurred in all experimental groups equally. Our results also showed a noticeable increase of Ascomycota proportion from NC, through CD to UC. Fungal genera Malassezia, Cladosporium and Toninia occurred in all experimental groups assuming that they are common components of the intestinal mycobiome. Other fungal genera found only in the NC experimental group were non-pathogenic and might bring some benefits. In contrast, CD and UC samples were characterized by an accumulation of genera with inhibitive effects on growth of other fungal genera and the presence of opportunistic pathogens. Furthermore, a decrease in the fungal genus Malassezia in inflammatory tissues was observed; Specifically, the UC experimental group showed a connection between the presence of Candida and seven time's lower amounts of Malassezia (compared to amounts found in NC). The CD experimental group was characterized by the simultaneous presence of Engyodontium album with Lecanicillium, and indicates a possible pathogenic effect of Ramularia in disease development.LAY SUMMARY: Mycobiome analysis of formalin-fixed and paraffin-embedded biopsies may highlight actual fungal genera composition in the intestinal wall. Interestingly, experimental groups of Crohn's disease and ulcerative colitis clearly differed by structure of their mycobiomes.
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    12
$a Ascomycota $7 D001203
650    _2
$a biopsie $7 D001706
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    12
$a idiopatické střevní záněty $x diagnóza $7 D015212
650    _2
$a střeva $7 D007422
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a lidé středního věku $7 D008875
650    12
$a mykobiom $7 D000072761
650    _2
$a mladý dospělý $7 D055815
655    _2
$a časopisecké články $7 D016428
700    1_
$a Riegert, Jan $u Department of Zoology, Faculty of Science, University of South Bohemia, 370 05 České Budějovice, Czech Republic $1 https://orcid.org/0000000341951278
700    1_
$a Kavková, Miloslava, $u Dairy Research Institute Ltd., Ke Dvoru 21a, Prague 160 00, Czech Republic $1 https://orcid.org/0000000170048315 $d 1970- $7 jcu2010587700
700    1_
$a Černá, Kateřina $u Department of Molecular Genetics, Bioptická Laboratoř s.r.o., 326 00 Pilsen, Czech Republic $1 https://orcid.org/000000034949565X
773    0_
$w MED00005131 $t Medical mycology $x 1460-2709 $g Roč. 60, č. 1 (2022)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34850076 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220425 $b ABA008
991    __
$a 20221102102014 $b ABA008
999    __
$a ok $b bmc $g 1789108 $s 1162559
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 60 $c 1 $e 2022Jan10 $i 1460-2709 $m Medical mycology $n Med Mycol $x MED00005131
LZP    __
$a Pubmed-20220425
Zpět

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace