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OMIP-080: 29-Color flow cytometry panel for comprehensive evaluation of NK and T cells reconstitution after hematopoietic stem cells transplantation
S. Vanikova, A. Koladiya, J. Musil
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
CZ.02.1.01/0.0/0.0/16_025/0007428 OP RDE MEYS
European Regional Development Fund and the state budget of the Czech Republic, project AIIHHP
Ministerstvo Zdravotnictví Ceské Republiky
IN 00023736
H CZ - DRO (Institute of Hematology and Blood Tranfusion - IHBT)
NLK
Free Medical Journals
from 2003 to 1 year ago
Medline Complete (EBSCOhost)
from 2012-06-01 to 1 year ago
Wiley Free Content
from 2003 to 1 year ago
PubMed
34693626
DOI
10.1002/cyto.a.24510
Knihovny.cz E-resources
- MeSH
- Hematopoietic Stem Cells MeSH
- Humans MeSH
- Lymphocyte Count MeSH
- Flow Cytometry MeSH
- T-Lymphocyte Subsets MeSH
- Hematopoietic Stem Cell Transplantation * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
This 29-color panel was developed and optimized for the monitoring of NK cell and T cell reconstitution in peripheral blood of patients after HSCT. We considered major post-HSCT complications during the design, such as relapses, viral infections, and GvHD and identification of lymphocyte populations relevant to their resolution. The panel includes markers for all major NK cell and T cell subsets and analysis of their development and qualitative properties. In the NK cell compartment, we focus mainly on CD57 + NKG2C+ cells and the expression of activating (NKG2D, DNAM-1) and inhibitory receptors (NKG2A, TIGIT). Another priority is the characterization of T cell reconstitution; therefore, we included detection of CD4+ RTEs based on CD45RA, CD62L, CD95, and CD31 as a marker of thymus function. Besides that, we also analyze the emergence and properties of major T cell populations with a particular interest in CD8, Th1, ThCTL, and Treg subsets. Overall, the panel allows for comprehensive analysis of the reconstituting immune system and identification of potential markers of immune cell dysfunction.
References provided by Crossref.org
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