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MALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration
Š. Strnad, V. Strnadová, D. Sýkora, J. Cvačka, L. Maletínská, V. Vrkoslav
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biomarkers MeSH
- Diagnostic Imaging MeSH
- Immunohistochemistry MeSH
- Lipids MeSH
- Brain * diagnostic imaging MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In mass spectrometry imaging (MSI), the essential steps in sample preparation include collection and storage. The most widely used preservation procedure for MSI consists in freezing samples and storing them at temperatures below -80 °C. On the other hand, the most common method for preserving biological samples in clinical practice is their fixation in paraformaldehyde. The storage of free-floating sections is a particular type of the preservation of paraformaldehyde-fixed tissues that is used in immunohistochemistry. This chapter describes the approach of the multimodal imaging of free-floating brain sections using the MSI of lipids and the immunohistochemistry of neurodegeneration markers.
References provided by Crossref.org
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