-
Je něco špatně v tomto záznamu ?
The intestinal microbiota and metabolites in patients with anorexia nervosa
P. Prochazkova, R. Roubalova, J. Dvorak, J. Kreisinger, M. Hill, H. Tlaskalova-Hogenova, P. Tomasova, H. Pelantova, M. Cermakova, M. Kuzma, J. Bulant, M. Bilej, K. Smitka, A. Lambertova, P. Holanova, H. Papezova
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Free Medical Journals od 2010 do Před 1 rokem
PubMed Central od 2010
Europe PubMed Central od 2010 do Před 1 rokem
Taylor & Francis Open Access od 2020-01-01
Medline Complete (EBSCOhost) od 2010-01-01
ROAD: Directory of Open Access Scholarly Resources od 2010
Odkazy
PubMed
33779487
DOI
10.1080/19490976.2021.1902771
Knihovny.cz E-zdroje
- MeSH
- Archaea klasifikace růst a vývoj MeSH
- Bacteria klasifikace růst a vývoj metabolismus MeSH
- dospělí MeSH
- feces mikrobiologie MeSH
- houby klasifikace růst a vývoj metabolismus MeSH
- index tělesné hmotnosti MeSH
- kyseliny mastné těkavé metabolismus MeSH
- lidé MeSH
- longitudinální studie MeSH
- mentální anorexie metabolismus mikrobiologie MeSH
- metagenom MeSH
- mladý dospělý MeSH
- mykobiom MeSH
- neurotransmiterové látky metabolismus MeSH
- osa mozek-střevo MeSH
- střevní mikroflóra * MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Brain-gut microbiota interactions are intensively studied in connection with various neurological and psychiatric diseases. While anorexia nervosa (AN) pathophysiology is not entirely clear, it is presumably linked to microbiome dysbiosis. We aimed to elucidate the gut microbiota contribution in AN disease pathophysiology. We analyzed the composition and diversity of the gut microbiome of patients with AN (bacteriome and mycobiome) from stool samples before and after renourishment, and compared them to healthy controls. Further, levels of assorted neurotransmitters and short-chain fatty acids (SCFA) were analyzed in stool samples by MS and NMR, respectively. Biochemical, anthropometric, and psychometric profiles were assessed. The bacterial alpha-diversity parameter analyses revealed only increased Chao 1 index in patients with AN before the realimentation, reflecting their interindividual variation. Subsequently, core microbiota depletion signs were observed in patients with AN. Overrepresented OTUs (operation taxonomic units) in patients with AN taxonomically belonged to Alistipes, Clostridiales, Christensenellaceae, and Ruminococcaceae. Underrepresented OTUs in patients with AN were Faecalibacterium, Agathobacter, Bacteroides, Blautia, and Lachnospira. Patients exhibited greater interindividual variation in the gut bacteriome, as well as in metagenome content compared to controls, suggesting altered bacteriome functions. Patients had decreased levels of serotonin, GABA, dopamine, butyrate, and acetate in their stool samples compared to controls. Mycobiome analysis did not reveal significant differences in alpha diversity and fungal profile composition between patients with AN and healthy controls, nor any correlation of the fungal composition with the bacterial profile. Our results show the changed profile of the gut microbiome and its metabolites in patients with severe AN. Although therapeutic partial renourishment led to increased body mass index and improved psychometric parameters, SCFA, and neurotransmitter profiles, as well as microbial community compositions, did not change substantially during the hospitalization period, which can be potentially caused by only partial weight recovery.
1st Faculty of Medicine Institute of Physiology Charles University Prague Czech Republic
Department of Steroids and Proteohormones Institute of Endocrinology Prague Czech Republic
Faculty of Science Department of Zoology Charles University Prague Czech Republic
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22012563
- 003
- CZ-PrNML
- 005
- 20220506130845.0
- 007
- ta
- 008
- 220425s2021 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1080/19490976.2021.1902771 $2 doi
- 035 __
- $a (PubMed)33779487
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Prochazkova, Petra $u Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic $1 https://orcid.org/000000028881971X $7 xx0128423
- 245 14
- $a The intestinal microbiota and metabolites in patients with anorexia nervosa / $c P. Prochazkova, R. Roubalova, J. Dvorak, J. Kreisinger, M. Hill, H. Tlaskalova-Hogenova, P. Tomasova, H. Pelantova, M. Cermakova, M. Kuzma, J. Bulant, M. Bilej, K. Smitka, A. Lambertova, P. Holanova, H. Papezova
- 520 9_
- $a Brain-gut microbiota interactions are intensively studied in connection with various neurological and psychiatric diseases. While anorexia nervosa (AN) pathophysiology is not entirely clear, it is presumably linked to microbiome dysbiosis. We aimed to elucidate the gut microbiota contribution in AN disease pathophysiology. We analyzed the composition and diversity of the gut microbiome of patients with AN (bacteriome and mycobiome) from stool samples before and after renourishment, and compared them to healthy controls. Further, levels of assorted neurotransmitters and short-chain fatty acids (SCFA) were analyzed in stool samples by MS and NMR, respectively. Biochemical, anthropometric, and psychometric profiles were assessed. The bacterial alpha-diversity parameter analyses revealed only increased Chao 1 index in patients with AN before the realimentation, reflecting their interindividual variation. Subsequently, core microbiota depletion signs were observed in patients with AN. Overrepresented OTUs (operation taxonomic units) in patients with AN taxonomically belonged to Alistipes, Clostridiales, Christensenellaceae, and Ruminococcaceae. Underrepresented OTUs in patients with AN were Faecalibacterium, Agathobacter, Bacteroides, Blautia, and Lachnospira. Patients exhibited greater interindividual variation in the gut bacteriome, as well as in metagenome content compared to controls, suggesting altered bacteriome functions. Patients had decreased levels of serotonin, GABA, dopamine, butyrate, and acetate in their stool samples compared to controls. Mycobiome analysis did not reveal significant differences in alpha diversity and fungal profile composition between patients with AN and healthy controls, nor any correlation of the fungal composition with the bacterial profile. Our results show the changed profile of the gut microbiome and its metabolites in patients with severe AN. Although therapeutic partial renourishment led to increased body mass index and improved psychometric parameters, SCFA, and neurotransmitter profiles, as well as microbial community compositions, did not change substantially during the hospitalization period, which can be potentially caused by only partial weight recovery.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a mentální anorexie $x metabolismus $x mikrobiologie $7 D000856
- 650 _2
- $a Archaea $x klasifikace $x růst a vývoj $7 D001105
- 650 _2
- $a Bacteria $x klasifikace $x růst a vývoj $x metabolismus $7 D001419
- 650 _2
- $a index tělesné hmotnosti $7 D015992
- 650 _2
- $a osa mozek-střevo $7 D000087502
- 650 _2
- $a kyseliny mastné těkavé $x metabolismus $7 D005232
- 650 _2
- $a feces $x mikrobiologie $7 D005243
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a houby $x klasifikace $x růst a vývoj $x metabolismus $7 D005658
- 650 12
- $a střevní mikroflóra $7 D000069196
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a longitudinální studie $7 D008137
- 650 _2
- $a metagenom $7 D054892
- 650 _2
- $a mykobiom $7 D000072761
- 650 _2
- $a neurotransmiterové látky $x metabolismus $7 D018377
- 650 _2
- $a mladý dospělý $7 D055815
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Roubalova, Radka $u Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic $1 https://orcid.org/0000000292984734
- 700 1_
- $a Dvorak, Jiri $u Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic $1 https://orcid.org/0000000237147781
- 700 1_
- $a Kreisinger, Jakub $u Faculty of Science, Department of Zoology, Charles University, Prague, Czech Republic $1 https://orcid.org/0000000193759814
- 700 1_
- $a Hill, Martin $u Department of Steroids and Proteohormones, Institute of Endocrinology, Prague, Czech Republic $1 https://orcid.org/0000000217050835 $7 mzk2005304431
- 700 1_
- $a Tlaskalova-Hogenova, Helena $u Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic $1 https://orcid.org/0000000303603563 $7 jn20000402365
- 700 1_
- $a Tomasova, Petra $u Laboratory of Molecular Structure Characterization, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic $u 4th Medical Department, First Faculty of Medicine, Charles University and General Faculty Hospital, Prague, Czech Republic $1 https://orcid.org/000000016886249X
- 700 1_
- $a Pelantova, Helena $u Laboratory of Molecular Structure Characterization, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic $1 https://orcid.org/0000000225237413
- 700 1_
- $a Cermakova, Martina $u Laboratory of Molecular Structure Characterization, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic $1 https://orcid.org/0000000249450630
- 700 1_
- $a Kuzma, Marek $u Laboratory of Molecular Structure Characterization, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic $1 https://orcid.org/0000000174152734
- 700 1_
- $a Bulant, Josef $u Department of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic $u Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic $1 https://orcid.org/0000000163360159 $7 xx0207735
- 700 1_
- $a Bilej, Martin $u Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic $1 https://orcid.org/0000000275243408
- 700 1_
- $a Smitka, Kvido $u First Faculty of Medicine, Institute of Physiology, Charles University, Prague, Czech Republic $u First Faculty of Medicine, Institute of Pathological Physiology, Charles University, Prague, Czech Republic $1 https://orcid.org/0000000198570543 $7 xx0139475
- 700 1_
- $a Lambertova, Alena $u Department of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic $1 https://orcid.org/0000000185564398 $7 xx0231937
- 700 1_
- $a Holanova, Petra $u Department of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic $1 https://orcid.org/000000020319941X $7 xx0233845
- 700 1_
- $a Papezova, Hana $u Department of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic $1 https://orcid.org/0000000181453295
- 773 0_
- $w MED00175586 $t Gut microbes $x 1949-0984 $g Roč. 13, č. 1 (2021), s. 1-25
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33779487 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20220425 $b ABA008
- 991 __
- $a 20220506130837 $b ABA008
- 999 __
- $a ok $b bmc $g 1789961 $s 1163764
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 13 $c 1 $d 1-25 $e - $i 1949-0984 $m Gut microbes $n Gut microbes $x MED00175586
- LZP __
- $a Pubmed-20220425