-
Je něco špatně v tomto záznamu ?
Genetic diversity of oral streptococci in the guinea pig as assessed by sequence analysis of the 16S rRNA and groEL genes
J. Król, A. Nowakiewicz, A. Błaszków, M. Brodala, A. Domagała, AN. Prassol, D. Sławska, J. Wojtynia
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Odkazy
PubMed
34932210
DOI
10.1007/s12223-021-00936-3
Knihovny.cz E-zdroje
- MeSH
- fylogeneze MeSH
- genetická variace * MeSH
- morčata MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- sekvenční analýza MeSH
- Streptococcus * genetika MeSH
- zvířata MeSH
- Check Tag
- morčata MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The aim of the present study was to characterize bacteria of the genus Streptococcus isolated from the oral cavity of the guinea pig as well as to assess the significance of these microorganisms as potential veterinary and human pathogens. Sixty-two streptococcal isolates recovered from 27 clinically healthy guinea pigs were examined genotypically by sequencing the 16S rRNA and groEL genes. Among these isolates, only 13 could be assigned to a species described previously (mainly Streptococcus parasanguinis, S. mitis and S. suis), and the majority of the remaining ones differed considerably from the streptococcal species known to date (16S rRNA and groEL sequence similarities were < 97% and < 87%, respectively). Based on 16S rRNA sequences, these unidentified isolates were divided into seven groups (clades), of which clades I through III comprised most of the isolates examined and had also the widest distribution among guinea pig colonies. Upon groEL gene sequence analysis, however, members of the three clades grouped together without forming such distinct clusters. The remaining clades distinguished by 16S rRNA sequencing could also be discerned by the second gene, and they contained only a few isolates often restricted to one or a few animal colonies. The present work reveals that the guinea pig mouth is inhabited by a vast number of phylogenetically diverse, so far unrecognized populations of streptococci, most of them being apparently host-specific genomospecies. On the contrary, S. parasanguinis and S. mitis are also common human commensals and S. suis is a well-recognized zoonotic pathogen.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22013098
- 003
- CZ-PrNML
- 005
- 20240416151236.0
- 007
- ta
- 008
- 220504s2022 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-021-00936-3 $2 doi
- 035 __
- $a (PubMed)34932210
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Król, Jarosław $u Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, St. Norwida 31, 50-375, Wrocław, Poland. jaroslaw.krol@upwr.edu.pl $1 https://orcid.org/0000000300124022
- 245 10
- $a Genetic diversity of oral streptococci in the guinea pig as assessed by sequence analysis of the 16S rRNA and groEL genes / $c J. Król, A. Nowakiewicz, A. Błaszków, M. Brodala, A. Domagała, AN. Prassol, D. Sławska, J. Wojtynia
- 520 9_
- $a The aim of the present study was to characterize bacteria of the genus Streptococcus isolated from the oral cavity of the guinea pig as well as to assess the significance of these microorganisms as potential veterinary and human pathogens. Sixty-two streptococcal isolates recovered from 27 clinically healthy guinea pigs were examined genotypically by sequencing the 16S rRNA and groEL genes. Among these isolates, only 13 could be assigned to a species described previously (mainly Streptococcus parasanguinis, S. mitis and S. suis), and the majority of the remaining ones differed considerably from the streptococcal species known to date (16S rRNA and groEL sequence similarities were < 97% and < 87%, respectively). Based on 16S rRNA sequences, these unidentified isolates were divided into seven groups (clades), of which clades I through III comprised most of the isolates examined and had also the widest distribution among guinea pig colonies. Upon groEL gene sequence analysis, however, members of the three clades grouped together without forming such distinct clusters. The remaining clades distinguished by 16S rRNA sequencing could also be discerned by the second gene, and they contained only a few isolates often restricted to one or a few animal colonies. The present work reveals that the guinea pig mouth is inhabited by a vast number of phylogenetically diverse, so far unrecognized populations of streptococci, most of them being apparently host-specific genomospecies. On the contrary, S. parasanguinis and S. mitis are also common human commensals and S. suis is a well-recognized zoonotic pathogen.
- 650 _2
- $a zvířata $7 D000818
- 650 12
- $a genetická variace $7 D014644
- 650 _2
- $a morčata $7 D006168
- 650 _2
- $a fylogeneze $7 D010802
- 650 _2
- $a RNA ribozomální 16S $x genetika $7 D012336
- 650 _2
- $a sekvenční analýza $7 D017421
- 650 _2
- $a sekvenční analýza DNA $7 D017422
- 650 12
- $a Streptococcus $x genetika $7 D013291
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Nowakiewicz, Aneta $u Sub-Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, St. Akademicka 12, 20-033, Lublin, Poland
- 700 1_
- $a Błaszków, Alicja $u Student of the Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wrocław, Poland
- 700 1_
- $a Brodala, Maria $u Student of the Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wrocław, Poland
- 700 1_
- $a Domagała, Adrianna $u Student of the Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wrocław, Poland
- 700 1_
- $a Prassol, Anna-Nicole $u Student of the Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wrocław, Poland
- 700 1_
- $a Sławska, Dominika $u Student of the Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wrocław, Poland
- 700 1_
- $a Wojtynia, Julita $u Student of the Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wrocław, Poland
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 67, č. 2 (2022), s. 311-318
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/34932210 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20220504 $b ABA008
- 991 __
- $a 20240416151233 $b ABA008
- 999 __
- $a ok $b bmc $g 1796136 $s 1164306
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2022 $b 67 $c 2 $d 311-318 $e 20211221 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- LZP __
- $a Pubmed-20220504