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Engineered Delivery of Dental Stem-Cell-Derived Extracellular Vesicles for Periodontal Tissue Regeneration
J. Zarubova, MM. Hasani-Sadrabadi, E. Dashtimoghadam, X. Zhang, S. Ansari, S. Li, A. Moshaverinia
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
Grantová podpora
R03 DE029876
NIDCR NIH HHS - United States
R56 DE029157
NIDCR NIH HHS - United States
DE029876
NIDCR NIH HHS - United States
1R56DE029157
NIDCR NIH HHS - United States
PubMed
35191610
DOI
10.1002/adhm.202102593
Knihovny.cz E-zdroje
- MeSH
- extracelulární vezikuly * MeSH
- kmenové buňky MeSH
- krysa rodu rattus MeSH
- mezenchymální kmenové buňky * MeSH
- nemoci parodontu * terapie MeSH
- parodont MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Periodontal disease begins as an inflammatory response to a bacterial biofilm deposited around the teeth, which over time leads to the destruction of tooth-supporting structures and consequently tooth loss. Conventional treatment strategies show limited efficacy in promoting regeneration of damaged periodontal tissues. Here, a delivery platform is developed for small extracellular vesicles (sEVs) derived from gingival mesenchymal stem cells (GMSCs) to treat periodontitis. EVs can achieve comparable therapeutic effects to their cells of origin. However, the short half-lives of EVs after their administration along with their rapid diffusion away from the delivery site necessitate frequent administration to achieve therapeutic benefits. To address these issues, "dual delivery" microparticles are engineered enabling microenvironment-sensitive release of EVs by metalloproteinases at the affected site along with antibiotics to suppress bacterial biofilm growth. GMSC sEVs are able to decrease the secretion of pro-inflammatory cytokines by monocytes/macrophages and T cells, suppress T-cell activation, and induce the formation of T regulatory cells (Tregs) in vitro and in a rat model of periodontal disease. One-time administration of immunomodulatory GMSC sEV-decorated microparticles leads to a significant improvement in regeneration of the damaged periodontal tissue. This approach will have potential clinical applications in the regeneration of a variety of tissues.
Citace poskytuje Crossref.org
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