Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Menopausal Transition: Prospective Study of Estrogen Status, Circulating MicroRNAs, and Biomarkers of Bone Metabolism

J. Baloun, A. Pekacova, L. Wenchich, H. Hruskova, L. Senolt, X. Svec, K. Pavelka, JJ. Stepan

. 2022 ; 13 (-) : 864299. [pub] 20220513

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc22018411

Objective: Osteoporosis is associated with an impaired balance between bone resorption and formation, which in turn leads to bone loss and fractures. Many recent studies have underlined the regulatory role of microRNAs (miRNAs) in bone remodeling processes and their potential as biomarkers of osteoporosis. The purpose of this study was to prospectively examine the association of circulating miRNAs and bone biomarkers with estrogen status in women before and after oophorectomy, as well as in oophorectomized women on estrogen therapy. Methods: In this prospective study, we included 11 women before oophorectomy and hysterectomy and at 201 ± 24 days after the surgery. Another 11 women were evaluated 508 ± 127 days after oophorectomy and hysterectomy and after an additional 203 ± 71 days of estradiol treatment. Serum miRNAs were profiled by sequencing. Estrogen status and biomarkers of bone metabolism were quantified. Bone mineral density was assessed in the lumbar spine. Results: Our analysis revealed 17 miRNAs associated with estrogen levels. Of those miRNAs that were upregulated with estrogen deficiency and downregulated after estrogen therapy, miR-422a correlated with serum beta-carboxy-terminal type I collagen crosslinks (β-CTX) and procollagen 1 N-terminal propeptide (P1NP); and miR-1278 correlated with serum β-CTX, P1NP, osteocalcin, sclerostin, and Dickkopf-1(Dkk1). In contrast, we found an inverse association of miR-24-1-5p with estrogen status and a negative correlation with serum β-CTX, P1NP, osteoprotegerin, and sclerostin levels. Conclusion: The reported miRNAs associated with estrogen status and bone metabolism could be potential biomarkers of bone pathophysiology and would facilitate studies on the prevention of postmenopausal osteoporosis. Our findings require validation in an extended cohort.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22018411
003      
CZ-PrNML
005      
20220804134745.0
007      
ta
008      
220720s2022 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3389/fendo.2022.864299 $2 doi
035    __
$a (PubMed)35634507
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Baloun, Jiri $u Institute of Rheumatology, Prague, Czechia $u Department of Rheumatology, First Faculty of Medicine, Charles University in Prague, Prague, Czechia
245    10
$a Menopausal Transition: Prospective Study of Estrogen Status, Circulating MicroRNAs, and Biomarkers of Bone Metabolism / $c J. Baloun, A. Pekacova, L. Wenchich, H. Hruskova, L. Senolt, X. Svec, K. Pavelka, JJ. Stepan
520    9_
$a Objective: Osteoporosis is associated with an impaired balance between bone resorption and formation, which in turn leads to bone loss and fractures. Many recent studies have underlined the regulatory role of microRNAs (miRNAs) in bone remodeling processes and their potential as biomarkers of osteoporosis. The purpose of this study was to prospectively examine the association of circulating miRNAs and bone biomarkers with estrogen status in women before and after oophorectomy, as well as in oophorectomized women on estrogen therapy. Methods: In this prospective study, we included 11 women before oophorectomy and hysterectomy and at 201 ± 24 days after the surgery. Another 11 women were evaluated 508 ± 127 days after oophorectomy and hysterectomy and after an additional 203 ± 71 days of estradiol treatment. Serum miRNAs were profiled by sequencing. Estrogen status and biomarkers of bone metabolism were quantified. Bone mineral density was assessed in the lumbar spine. Results: Our analysis revealed 17 miRNAs associated with estrogen levels. Of those miRNAs that were upregulated with estrogen deficiency and downregulated after estrogen therapy, miR-422a correlated with serum beta-carboxy-terminal type I collagen crosslinks (β-CTX) and procollagen 1 N-terminal propeptide (P1NP); and miR-1278 correlated with serum β-CTX, P1NP, osteocalcin, sclerostin, and Dickkopf-1(Dkk1). In contrast, we found an inverse association of miR-24-1-5p with estrogen status and a negative correlation with serum β-CTX, P1NP, osteoprotegerin, and sclerostin levels. Conclusion: The reported miRNAs associated with estrogen status and bone metabolism could be potential biomarkers of bone pathophysiology and would facilitate studies on the prevention of postmenopausal osteoporosis. Our findings require validation in an extended cohort.
650    _2
$a biologické markery $7 D015415
650    _2
$a estrogeny $7 D004967
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a menopauza $7 D008593
650    12
$a mikro RNA $x genetika $7 D035683
650    12
$a osteoporóza $7 D010024
650    _2
$a prospektivní studie $7 D011446
655    _2
$a časopisecké články $7 D016428
700    1_
$a Pekacova, Aneta $u Institute of Rheumatology, Prague, Czechia $u Department of Rheumatology, First Faculty of Medicine, Charles University in Prague, Prague, Czechia
700    1_
$a Wenchich, Laszlo $u Institute of Rheumatology, Prague, Czechia
700    1_
$a Hruskova, Hana $u Department of Obstetrics and Gynecology, First Faculty of Medicine, Charles University in Prague, Prague, Czechia $u General University Hospital in Prague, Prague, Czechia
700    1_
$a Senolt, Ladislav $u Institute of Rheumatology, Prague, Czechia $u Department of Rheumatology, First Faculty of Medicine, Charles University in Prague, Prague, Czechia
700    1_
$a Svec, Xiao $u Institute of Rheumatology, Prague, Czechia
700    1_
$a Pavelka, Karel $u Institute of Rheumatology, Prague, Czechia $u Department of Rheumatology, First Faculty of Medicine, Charles University in Prague, Prague, Czechia
700    1_
$a Stepan, Jan J $u Institute of Rheumatology, Prague, Czechia $u Department of Rheumatology, First Faculty of Medicine, Charles University in Prague, Prague, Czechia
773    0_
$w MED00174543 $t Frontiers in endocrinology $x 1664-2392 $g Roč. 13, č. - (2022), s. 864299
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35634507 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220720 $b ABA008
991    __
$a 20220804134738 $b ABA008
999    __
$a ok $b bmc $g 1822145 $s 1169654
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 13 $c - $d 864299 $e 20220513 $i 1664-2392 $m Frontiers in endocrinology $n Front. endocrinol. $x MED00174543
LZP    __
$a Pubmed-20220720

Find record

Citation metrics

Archiving options