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Coating Ti6Al4V implants with nanocrystalline diamond functionalized with BMP-7 promotes extracellular matrix mineralization in vitro and faster osseointegration in vivo

I. Nemcakova, A. Litvinec, V. Mandys, S. Potocky, M. Plencner, M. Doubkova, O. Nanka, V. Olejnickova, B. Sankova, M. Bartos, E. Ukraintsev, O. Babčenko, L. Bacakova, A. Kromka, B. Rezek, D. Sedmera

. 2022 ; 12 (1) : 5264. [pub] 20220328

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc22019116

The present study investigates the effect of an oxidized nanocrystalline diamond (O-NCD) coating functionalized with bone morphogenetic protein 7 (BMP-7) on human osteoblast maturation and extracellular matrix mineralization in vitro and on new bone formation in vivo. The chemical structure and the morphology of the NCD coating and the adhesion, thickness and morphology of the superimposed BMP-7 layer have also been assessed. The material analysis proved synthesis of a conformal diamond coating with a fine nanostructured morphology on the Ti6Al4V samples. The homogeneous nanostructured layer of BMP-7 on the NCD coating created by a physisorption method was confirmed by AFM. The osteogenic maturation of hFOB 1.19 cells in vitro was only slightly enhanced by the O-NCD coating alone without any increase in the mineralization of the matrix. Functionalization of the coating with BMP-7 resulted in more pronounced cell osteogenic maturation and increased extracellular matrix mineralization. Similar results were obtained in vivo from micro-CT and histological analyses of rabbit distal femurs with screws implanted for 4 or 12 weeks. While the O-NCD-coated implants alone promoted greater thickness of newly-formed bone in direct contact with the implant surface than the bare material, a further increase was induced by BMP-7. It can be therefore concluded that O-NCD coating functionalized with BMP-7 is a promising surface modification of metallic bone implants in order to improve their osseointegration.

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$a Coating Ti6Al4V implants with nanocrystalline diamond functionalized with BMP-7 promotes extracellular matrix mineralization in vitro and faster osseointegration in vivo / $c I. Nemcakova, A. Litvinec, V. Mandys, S. Potocky, M. Plencner, M. Doubkova, O. Nanka, V. Olejnickova, B. Sankova, M. Bartos, E. Ukraintsev, O. Babčenko, L. Bacakova, A. Kromka, B. Rezek, D. Sedmera
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$a The present study investigates the effect of an oxidized nanocrystalline diamond (O-NCD) coating functionalized with bone morphogenetic protein 7 (BMP-7) on human osteoblast maturation and extracellular matrix mineralization in vitro and on new bone formation in vivo. The chemical structure and the morphology of the NCD coating and the adhesion, thickness and morphology of the superimposed BMP-7 layer have also been assessed. The material analysis proved synthesis of a conformal diamond coating with a fine nanostructured morphology on the Ti6Al4V samples. The homogeneous nanostructured layer of BMP-7 on the NCD coating created by a physisorption method was confirmed by AFM. The osteogenic maturation of hFOB 1.19 cells in vitro was only slightly enhanced by the O-NCD coating alone without any increase in the mineralization of the matrix. Functionalization of the coating with BMP-7 resulted in more pronounced cell osteogenic maturation and increased extracellular matrix mineralization. Similar results were obtained in vivo from micro-CT and histological analyses of rabbit distal femurs with screws implanted for 4 or 12 weeks. While the O-NCD-coated implants alone promoted greater thickness of newly-formed bone in direct contact with the implant surface than the bare material, a further increase was induced by BMP-7. It can be therefore concluded that O-NCD coating functionalized with BMP-7 is a promising surface modification of metallic bone implants in order to improve their osseointegration.
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$a Litvinec, Andrej $u Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic
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$a Mandys, Vaclav $u Department of Pathology, Charles University, Third Faculty of Medicine, Ruska 2411, 100 00, Prague 10, Czech Republic
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$a Potocky, Stepan $u Institute of Physics, Czech Academy of Sciences, Cukrovarnicka 10, 162 00, Prague 6, Czech Republic
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$a Plencner, Martin $u Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic
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$a Doubkova, Martina $u Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic. martina.doubkova@fgu.cas.cz
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$a Nanka, Ondrej $u Institute of Anatomy, Charles University, First Faculty of Medicine, U Nemocnice 3, 128 00, Prague 2, Czech Republic
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$a Olejnickova, Veronika $u Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic $u Institute of Anatomy, Charles University, First Faculty of Medicine, U Nemocnice 3, 128 00, Prague 2, Czech Republic
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$a Sankova, Barbora $u Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic $u Institute of Anatomy, Charles University, First Faculty of Medicine, U Nemocnice 3, 128 00, Prague 2, Czech Republic
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$a Bartos, Martin $u Institute of Dental Medicine, Charles University, First Faculty of Medicine, U Nemocnice 2, 1280 00, Prague 2, Czech Republic
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$a Ukraintsev, Egor $u Faculty of Electrical Engineering, Czech Technical University in Prague, Technicka 2, 166 27, Prague 6, Czech Republic
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$a Babčenko, Oleg $u Faculty of Electrical Engineering, Czech Technical University in Prague, Technicka 2, 166 27, Prague 6, Czech Republic
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$a Bacakova, Lucie $u Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic
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$a Kromka, Alexander $u Institute of Physics, Czech Academy of Sciences, Cukrovarnicka 10, 162 00, Prague 6, Czech Republic
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$a Rezek, Bohuslav $u Faculty of Electrical Engineering, Czech Technical University in Prague, Technicka 2, 166 27, Prague 6, Czech Republic
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