Objectives: The goal of this study was to see how systemic vitamin D3 supplementation affected the durability of dental implants as assessed by radiofrequency analysis.Methods: This split-mouth clinical trial included a total of twelve patients seeking dental implant therapy, ranging in age from 25 to 50 years. The patients were divided into control group and treatment group. Each patient in both groups received dental implants in posterior maxillary extraction sites. The radiofrequency analysis (RFA) was conducted using Osstell Mentor device to evaluate the stability of dental implant at various time points.Results: During the three months of the trial, substantial differences between the treatment and control groups were detected according to osstell meter device at the time of insertion (primary stability) and 3 months later (secondary stability).Conclusion: Vitamin D supplementation has a positive effect on dental implant stability.
Biocompatibility is one of the key issues for implants, especially in the case of stainless steel with medium to low biocompatibility, which may lead to a lack of osseointegration and consequently to implant failure or rejection. To precisely control preferential cell growth sites and, consequently, the biocompatibility of prosthetic devices, two types of surfaces were analyzed, containing periodic nanogrooves laser induced periodic surface structure (LIPSS) and square-shaped micropillars. For the fast and efficient production of these surfaces, the unique combination of high energy ultrashort pulsed laser system with multi-beam and beamshaping technology was applied, resulting in increased productivity by 526% for micropillars and 14 570% for LIPSS compared to single beam methods.In vitroanalysis revealed that micro and nanostructured surfaces provide a better environment for cell attachment and proliferation compared to untreated ones, showing an increase of up to 496% in the number of cells compared to the reference. Moreover, the combination of LIPSS and micropillars resulted in a precise cell orientation along the periodic microgroove pattern. The combination of these results demonstrates the possibility of mass production of functionalized implants with control over cell organization and growth. Thus, reducing the risk of implant failure due to low biocompatibility.
- MeSH
- implantace zubů MeSH
- kongresy jako téma MeSH
- lidé MeSH
- osteointegrace * MeSH
- zubní implantáty MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- zprávy MeSH
The present study investigates the effect of an oxidized nanocrystalline diamond (O-NCD) coating functionalized with bone morphogenetic protein 7 (BMP-7) on human osteoblast maturation and extracellular matrix mineralization in vitro and on new bone formation in vivo. The chemical structure and the morphology of the NCD coating and the adhesion, thickness and morphology of the superimposed BMP-7 layer have also been assessed. The material analysis proved synthesis of a conformal diamond coating with a fine nanostructured morphology on the Ti6Al4V samples. The homogeneous nanostructured layer of BMP-7 on the NCD coating created by a physisorption method was confirmed by AFM. The osteogenic maturation of hFOB 1.19 cells in vitro was only slightly enhanced by the O-NCD coating alone without any increase in the mineralization of the matrix. Functionalization of the coating with BMP-7 resulted in more pronounced cell osteogenic maturation and increased extracellular matrix mineralization. Similar results were obtained in vivo from micro-CT and histological analyses of rabbit distal femurs with screws implanted for 4 or 12 weeks. While the O-NCD-coated implants alone promoted greater thickness of newly-formed bone in direct contact with the implant surface than the bare material, a further increase was induced by BMP-7. It can be therefore concluded that O-NCD coating functionalized with BMP-7 is a promising surface modification of metallic bone implants in order to improve their osseointegration.
- MeSH
- biokompatibilní potahované materiály chemie farmakologie MeSH
- diamant chemie MeSH
- extracelulární matrix MeSH
- kostní morfogenetický protein 7 * farmakologie MeSH
- králíci MeSH
- osteointegrace * MeSH
- slitiny MeSH
- titan MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
ÚVOD: Zlomeniny stehenní kosti jsou nejčastěji výsledkem vysokoenergetického mechanizmu. Jejich primární ošetření, definitivní řešení a řešení poruch hojení jsou stále námětem k diskuzi. CÍL: Cílem práce bylo analyzovat příčiny poruchy kostního hojení stehenní kosti, infekční komplikace a možnosti jejich řešení. METODIKA: Jedná se o retrospektivní studii 1433 pacientů z let 2014 až 2018. Zaměřili jsme se na skupinu sdruženě poraněných pacientů s NISS > 15, kteří zároveň utrpěli zlomeninu diafýzy nebo distální části stehenní kosti. Z tohoto souboru 178 pacientů zemřelo. Ze zbylých 998 pacientů s NISS > 15 utrpělo 82 pacientů (8 %) současně zlomeninu diafyzární nebo distální části stehenní kosti. VÝSLEDKY: Ke zhojení zlomeniny bez nutnosti další intervence došlo v 84 % případů. U dvou pacientů (2,6 %) byl stav hodnocen jako prodloužené hojení. K primárnímu nezhojení došlo celkem u 6 pacientů (8 %). DISKUZE: K řešení poruchy hojení stehenní kosti se nejčastěji používá výměna hřebu za silnější po předvrtání dřeňové dutiny a s možným přidáním vymezovacích šroubů. Úspěšnost této techniky se pohybuje mezi 75 a 100 %. Další možnou operační technikou je augmentace stávajícího hřebu přídatnou dlahou, která se osvědčila zejména v infraistmické části diafýzy, tedy v distální třetině. Úspěšnost této metody překračuje 90 %. Třetí možnou operační technikou je konverze nitrodřeňové osteosyntézy na dlahovou. Její význam spatřujeme při nutné konverzi relativní na absolutní stabilitu. ZÁVĚR: Příčiny poruchy hojení kosti jsou mechanické, biologické a kombinace obojího. Mechanickou příčinou může být nedostatečná stabilita nebo naopak přílišná rigidita osteosyntézy, která nevede ke tvorbě svalku. Biologickou příčinou je nejčastěji porucha výživy fragmentů zlomeniny. Odhalením příčiny poruchy hojení pak můžeme správnou intervencí dosáhnout zhojení.
- MeSH
- diafýzy chirurgie MeSH
- fraktury femuru chirurgie etiologie MeSH
- hojení fraktur MeSH
- hojení ran MeSH
- lidé MeSH
- mnohočetné fraktury chirurgie MeSH
- osteointegrace MeSH
- pooperační komplikace diagnostické zobrazování epidemiologie etiologie MeSH
- retrospektivní studie MeSH
- vnitřní fixace fraktury metody přístrojové vybavení statistika a číselné údaje MeSH
- Check Tag
- lidé MeSH
INTRODUCTION: Femoral fractures are most often the result of a high-energy mechanism. Their primary treatment, definitive management and addressing of impaired healing are still a matter of discussion. AIM: The aim of the study was to analyse the causes of impaired healing of femur, infectious complications and their possible solutions. METHODOLOGY: It is a retrospective study of 1,433 patients from years 2014 to 2018. We focused on a group of polytrauma patients with NISS > 15, who also suffered a diaphyseal or distal femur fracture. Of this cohort, 178 patients died. Of the remaining 998 patients with NISS > 15, 82 patients (8 %) suffered a concomitant diaphyseal or distal femur fracture. RESULTS: The fracture healed without the need for further intervention in 84 % of cases. In two patients (2.6 %), the condition was assessed as prolonged healing. Primary non-healing occurred in a total of 6 patients (8 %). DISCUSSION: Replacement of the nail with a thicker nail after reaming the medullary cavity and with the possible addition of positional screws is most commonly used to manage the impaired healing of femur. The success rate of this technique ranges from 75 to 100 %. Another possible surgical technique is the augmentation of the existing nail with an additional plate, which has proven to be particularly successful in the infraisthmic portion of the diaphysis, i.e. in the distal third. The success rate of this method exceeds 90 %. The third possible surgical technique is a conversion of intra-articular osteosynthesis to plate osteosynthesis. Its importance is seen in the necessary conversion of relative to absolute stability. CONCLUSION: The causes of impaired bone healing are mechanical, biological, and a combination of both. The mechanical cause may be insufficient stability or, on the contrary, excessive rigidity of the osteosynthesis, which does not result in muscle formation. The biological cause is most often a malnutrition of the fracture fragments. Then, by discovering the cause of the impaired healing, we can achieve healing with the right intervention.
- MeSH
- diafýzy MeSH
- fraktury femuru * chirurgie etiologie MeSH
- hojení fraktur MeSH
- hojení ran MeSH
- lidé MeSH
- osteointegrace MeSH
- polytrauma MeSH
- pooperační komplikace epidemiologie etiologie MeSH
- retrospektivní studie MeSH
- vnitřní fixace fraktury metody přístrojové vybavení statistika a číselné údaje MeSH
- Check Tag
- lidé MeSH
Titanium surface treatment is a crucial process for achieving sufficient osseointegration of an implant into the bone. If the implant does not heal sufficiently, serious complications may occur, e.g. infection, inflammation, aseptic loosening of the implant, or the stress-shielding effect, as a result of which the implant may need to be reoperated. After a titanium graft has been implanted, several interactions are crucial in order to create a strong bone-implant connection. It is essential that cells adhere to the surface of the implant. Surface roughness has a significant influence on cell adhesion, and also on improving and accelerating osseointegration. Other highly important factors are biocompatibility and resistance to bacterial contamination. Bio-inertness of titanium is ensured by the protective film of titanium oxides that forms spontaneously on its surface. This film prevents the penetration of metal compounds, and it is well-adhesive for calcium and phosphate ions, which are necessary for the formation of the mineralized bone structure. Since the presence of the film alone is not sufficient for the biocompatibility of titanium, a suitable surface finish is required to create a firm bone-implant connection. In this review, we explain and compare the most widely-used methods for modulating the surface roughness of titanium implants in order to enhance cell adhesion on the surface of the implant, e.g. plasma spraying, sandblasting, acid etching, laser treatment, sol-gel etc., The methods are divided into three overlapping groups, according to the type of modification.
- MeSH
- biokompatibilní potahované materiály * MeSH
- lidé MeSH
- osteointegrace * MeSH
- protézy a implantáty * MeSH
- titan * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Dental implant dimensions, and bone quality and quantity play a key role in early osseointegration and long-term prognosis in posterior edentulous maxilla. Treatment with short implants, preferably in a bicortical manner, is an accepted modality; however, short implants have limitations leading to increased stress concentrations in alveolar bone, potential overload and implant failure. Implant models of 3.3, 4.1, 4.8 and 5.4 mm diameter and 4.5, 5.5, 6.5, 7.5 and 8.5 mm length were placed in posterior maxilla 3-D models with corresponding residual bone heights. Bone-implant assemblies were analyzed in finite element software ANSYS 15. All materials were assumed to be linearly elastic and isotropic. 118.2 N oblique loading was applied to investigate stress distributions in bone tissues. The concept of ultimate functional load (UFL) was selected as a criterion to compare load-carrying capacity of implants and to evaluate the influence of available bone height and implant dimensions on load-carrying capacity. For all implants, UFL was calculated by limiting von Mises stresses in cortical or cancellous bone with bone strength (100 MPa for cortical and 2 MPa for cancellous bone). Implant load-carrying capacity depends on diameter and available bone height. Wide implants have higher load-carrying capacity than narrow implants. Short implants with proper diameter and length avoid bone overstress, even in Type IV bone.
Fabrication of porous and biologically inspired biomaterials that mimic the formation of microstructural structures of nacre in the form of calcite (CaCO3) and evaluation of the biocompatibility of such organic-inorganic composite scaffold for bone tissue engineering, are focus of this paper. Nacre's self-assembly characteristics are concerned about the development of calcite filled biomineralized scaffold following the nature based biomineralization process and biomimetic applications. The PVP-CMC hydrogel film, comprised of PVP:0.2, CMC:0.8, PEG:1.0, Agar:2.0, Glycerene:1.0 and water:95.0 w/v%; acts as catalyst and template for the nucleation and growth of the inorganic CaCO3 within the scaffold. The PVP-CMC hydrogel (in the dry state) was immersed in ionic solutions (g/100 ml) of Na2CO3 and CaCl2·H2O in different concentrations sets i.e. Set-1: 10.50/14.70; Set-2: 5.25/7.35; Set-3: 4.20/5.88; Set-4: 2.10/2.94; Set-5: 1.05/1.47, Set-6: 0.55/0.55 for 90 min. As a result, "PVP-CMC-CaCO3" hydrogel scaffold was fabricated having bio-inspired structural and functional properties. Cell proliferation and cell viability were examined until 7 days in the presence of "PVP-CMC-CaCO3" scaffolds using permanent cell lines MG63 (human osteosarcoma), L929 (murine fibroblasts) as well as cultures from mouse bone explants (CC-MBE), confirmed that the said hydrogel scaffolds are biocompatible. But, from mechanical strength as well as biocompatibility point of view, scaffolds prepared in Set-1 to 3 ionic solutions were superior. In conclusion, these three calcite filled hydrogel scaffolds are recommended and can be used for osseointegration.
- MeSH
- biokompatibilní materiály chemie MeSH
- buněčné linie MeSH
- difrakce rentgenového záření MeSH
- hydrogely chemie MeSH
- mikroskopie elektronová rastrovací MeSH
- myši MeSH
- osteointegrace fyziologie MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury chemie MeSH
- viabilita buněk fyziologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The objectives of this study were to evaluate osseointegration of dental implant in the jaw bone in the young and elderly population and comparing the results to assess indicators and risk factors as age for the success or failure of dental implants. A retrospective study of 107 implants (Impladent, LASAK, Czech Republic) was prepared. The patients at implants surgery were divided in three groups. The patients were followed-up for a 7-year period. We evaluated osseointegration from long term point of view as a change of marginal bone levels close to dental implant. Marginal bone levels were recorded and analysed with regard to different patient- and implant-related factors. An influence of chronological age on change of marginal bone levels during 6-year retrospective study vas evaluated. The study examined 47 patient charts and 107 implants from the Second Faculty of Medicine, Charles University and University Hospital Motol. We proved that young healthy patients with long bridges or Branemarks have the same progression of marginal bone levels changes. The chronological age hasn't therefore direct influence on the osseointegration from long term point of view. But we found that the length of dental suprastrucure-prosthetic construction negatively influences marginal bone changes, though these results weren't statistically significant. More extensive dental implant suprastrucure undergoes smaller osseointegration. On the other hand the length of dental suprastrucure (prosthetic construction) negatively influences dental osseointegration in both groups of patient.
- MeSH
- čelist bezzubá diagnóza MeSH
- dospělí MeSH
- endoseální implantace zubů statistika a číselné údaje MeSH
- lidé středního věku MeSH
- lidé MeSH
- následné studie MeSH
- osteointegrace fyziologie MeSH
- retrospektivní studie MeSH
- výsledek terapie MeSH
- zubní implantáty statistika a číselné údaje MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
