Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction

V. Zywitza, E. Rusha, D. Shaposhnikov, J. Ruiz-Orera, N. Telugu, V. Rishko, M. Hayashi, G. Michel, L. Wittler, J. Stejskal, S. Holtze, F. Göritz, R. Hermes, J. Wang, Z. Izsvák, S. Colleoni, G. Lazzari, C. Galli, TB. Hildebrandt, K. Hayashi, S....

. 2022 ; 12 (1) : 3100. [pub] 20220308

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22019218

The northern white rhinoceros (NWR) is probably the earth's most endangered mammal. To rescue the functionally extinct species, we aim to employ induced pluripotent stem cells (iPSCs) to generate gametes and subsequently embryos in vitro. To elucidate the regulation of pluripotency and differentiation of NWR PSCs, we generated iPSCs from a deceased NWR female using episomal reprogramming, and observed surprising similarities to human PSCs. NWR iPSCs exhibit a broad differentiation potency into the three germ layers and trophoblast, and acquire a naïve-like state of pluripotency, which is pivotal to differentiate PSCs into primordial germ cells (PGCs). Naïve culturing conditions induced a similar expression profile of pluripotency related genes in NWR iPSCs and human ESCs. Furthermore, naïve-like NWR iPSCs displayed increased expression of naïve and PGC marker genes, and a higher integration propensity into developing mouse embryos. As the conversion process was aided by ectopic BCL2 expression, and we observed integration of reprogramming factors, the NWR iPSCs presented here are unsuitable for gamete production. However, the gained insights into the developmental potential of both primed and naïve-like NWR iPSCs are fundamental for in future PGC-specification in order to rescue the species from extinction using cryopreserved somatic cells.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22019218
003      
CZ-PrNML
005      
20220804135437.0
007      
ta
008      
220720s2022 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-022-07059-w $2 doi
035    __
$a (PubMed)35260583
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Zywitza, Vera $u Technology Platform Pluripotent Stem Cells, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125, Berlin, Germany
245    10
$a Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction / $c V. Zywitza, E. Rusha, D. Shaposhnikov, J. Ruiz-Orera, N. Telugu, V. Rishko, M. Hayashi, G. Michel, L. Wittler, J. Stejskal, S. Holtze, F. Göritz, R. Hermes, J. Wang, Z. Izsvák, S. Colleoni, G. Lazzari, C. Galli, TB. Hildebrandt, K. Hayashi, S. Diecke, M. Drukker
520    9_
$a The northern white rhinoceros (NWR) is probably the earth's most endangered mammal. To rescue the functionally extinct species, we aim to employ induced pluripotent stem cells (iPSCs) to generate gametes and subsequently embryos in vitro. To elucidate the regulation of pluripotency and differentiation of NWR PSCs, we generated iPSCs from a deceased NWR female using episomal reprogramming, and observed surprising similarities to human PSCs. NWR iPSCs exhibit a broad differentiation potency into the three germ layers and trophoblast, and acquire a naïve-like state of pluripotency, which is pivotal to differentiate PSCs into primordial germ cells (PGCs). Naïve culturing conditions induced a similar expression profile of pluripotency related genes in NWR iPSCs and human ESCs. Furthermore, naïve-like NWR iPSCs displayed increased expression of naïve and PGC marker genes, and a higher integration propensity into developing mouse embryos. As the conversion process was aided by ectopic BCL2 expression, and we observed integration of reprogramming factors, the NWR iPSCs presented here are unsuitable for gamete production. However, the gained insights into the developmental potential of both primed and naïve-like NWR iPSCs are fundamental for in future PGC-specification in order to rescue the species from extinction using cryopreserved somatic cells.
650    _2
$a zvířata $7 D000818
650    _2
$a buněčná diferenciace $x genetika $7 D002454
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a zárodečné buňky $x metabolismus $7 D005854
650    _2
$a zárodečné listy $7 D005855
650    12
$a indukované pluripotentní kmenové buňky $7 D057026
650    _2
$a myši $7 D051379
650    _2
$a Perissodactyla $x genetika $7 D010527
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Rusha, Ejona $u Induced Pluripotent Stem Cell Core Facility, Helmholtz Zentrum München, 85764, Neuherberg, Germany
700    1_
$a Shaposhnikov, Dmitry $u Induced Pluripotent Stem Cell Core Facility, Helmholtz Zentrum München, 85764, Neuherberg, Germany
700    1_
$a Ruiz-Orera, Jorge $u Cardiovascular and Metabolic Sciences, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125, Berlin, Germany
700    1_
$a Telugu, Narasimha $u Technology Platform Pluripotent Stem Cells, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125, Berlin, Germany
700    1_
$a Rishko, Valentyna $u Induced Pluripotent Stem Cell Core Facility, Helmholtz Zentrum München, 85764, Neuherberg, Germany
700    1_
$a Hayashi, Masafumi $u Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-8582, Japan
700    1_
$a Michel, Geert $u FEMTransgenic Technologies, Charité, 13125, Berlin, Germany
700    1_
$a Wittler, Lars $u Department of Developmental Genetics, Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany
700    1_
$a Stejskal, Jan $u ZOO Dvůr Králové, Štefánikova 1029, 544 01, Dvůr Králové nad Labem, Czech Republic
700    1_
$a Holtze, Susanne $u Leibniz Institute for Zoo and Wildlife Research, 10315, Berlin, Germany
700    1_
$a Göritz, Frank $u Leibniz Institute for Zoo and Wildlife Research, 10315, Berlin, Germany
700    1_
$a Hermes, Robert $u Leibniz Institute for Zoo and Wildlife Research, 10315, Berlin, Germany
700    1_
$a Wang, Jichang $u Mobile DNA, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125, Berlin, Germany
700    1_
$a Izsvák, Zsuzsanna $u Mobile DNA, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125, Berlin, Germany
700    1_
$a Colleoni, Silvia $u Laboratory of Reproductive Technologies, Avantea, 26100, Cremona, Italy
700    1_
$a Lazzari, Giovanna $u Laboratory of Reproductive Technologies, Avantea, 26100, Cremona, Italy $u Fondazione Avantea, 26100, Cremona, Italy
700    1_
$a Galli, Cesare $u Laboratory of Reproductive Technologies, Avantea, 26100, Cremona, Italy $u Fondazione Avantea, 26100, Cremona, Italy
700    1_
$a Hildebrandt, Thomas B $u Leibniz Institute for Zoo and Wildlife Research, 10315, Berlin, Germany $u Faculty of Veterinary Medicine, Freie Universität Berlin, 14163, Berlin, Germany
700    1_
$a Hayashi, Katsuhiko $u Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-8582, Japan
700    1_
$a Diecke, Sebastian $u Technology Platform Pluripotent Stem Cells, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125, Berlin, Germany. Sebastian.Diecke@mdc-berlin.de
700    1_
$a Drukker, Micha $u Induced Pluripotent Stem Cell Core Facility, Helmholtz Zentrum München, 85764, Neuherberg, Germany. M.Drukker@lacdr.leidenuniv.nl $u Division of Drug Discovery and Safety, Leiden Academic Centre for Drug Research (LACDR), Leiden University, 2300 RA, Leiden, The Netherlands. M.Drukker@lacdr.leidenuniv.nl
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 12, č. 1 (2022), s. 3100
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35260583 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220720 $b ABA008
991    __
$a 20220804135430 $b ABA008
999    __
$a ok $b bmc $g 1822702 $s 1170461
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 12 $c 1 $d 3100 $e 20220308 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
LZP    __
$a Pubmed-20220720

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace