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Retinoic Acid Grafted to Hyaluronic Acid Activates Retinoid Gene Expression and Removes Cholesterol from Cellular Membranes
V. Pavlík, V. Machalová, M. Čepa, R. Šínová, B. Šafránková, J. Kulhánek, T. Drmota, L. Kubala, G. Huerta-Ángeles, V. Velebný, K. Nešporová
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed Central od 2011
Europe PubMed Central od 2011
ProQuest Central od 2011-01-01
Open Access Digital Library od 2011-01-01
Open Access Digital Library od 2011-01-01
Health & Medicine (ProQuest) od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources od 2011
Odkazy
PubMed
35204701
DOI
10.3390/biom12020200
Knihovny.cz E-zdroje
- MeSH
- cholesterol metabolismus MeSH
- exprese genu MeSH
- kyselina hyaluronová farmakologie MeSH
- retinoidy * farmakologie MeSH
- tretinoin * farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
All-trans-retinoic acid (atRA) is a potent ligand that regulates gene expression and is used to treat several skin disorders. Hyaluronic acid (HA) was previously conjugated with atRA (HA-atRA) to obtain a novel amphiphilic compound. HA-atRA forms micelles that incorporate hydrophobic molecules and facilitate their transport through the skin. The aim of this study was to determine the influence of HA-atRA on gene expression in skin cells and to compare it with that of unbound atRA. Gene expression was investigated using microarrays and a luciferase system with a canonical atRA promoter. HA-atRA upregulated gene expression similarly to atRA. However, HA-atRA activated the expression of cholesterol metabolism genes, unlike atRA. Further investigation using HPLC and filipin III staining suggested that the treated cells induced cholesterol synthesis to replenish the cholesterol removed from the cells by HA-atRA. HA modified with oleate (HA-C18:1) removed cholesterol from the cells similarly to HA-atRA, suggesting that the cholesterol removal stemmed from the amphiphilic nature of the two derivatives. HA-atRA induces retinoid signaling. Thus, HA-atRA could be used to treat skin diseases, such as acne and psoriasis, where the combined action of atRA signaling and anti-inflammatory cholesterol removal may be potentially beneficial.
3rd Faculty of Medicine Charles University 100 00 Prague Czech Republic
Institute of Experimental Biology Faculty of Science Masaryk University 625 00 Brno Czech Republic
International Clinical Research Center St Anne's University Hospital 656 91 Brno Czech Republic
R and D Department Contipro a s 562 04 Dolní Dobrouč Czech Republic
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