• Something wrong with this record ?

Comparaison des approches de diagnostic moléculaire pour la détection et la différenciation du protiste intestinal Blastocystis sp. chez l'homme [Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans]

M. Šloufová, Z. Lhotská, M. Jirků, KJ. Petrželková, CR. Stensvold, O. Cinek, KJ. Pomajbíková

. 2022 ; 29 (-) : 30. [pub] 20220531

Language English Country France

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc22019582

Grant support
RGY0078/2015 Human Frontier Science Program
22-04837S Grantová Agentura České Republiky
00064203 Ministerstvo Zdravotnictví Ceské Republiky

Blastocystis is the most commonly found intestinal protist in the world. Accurate detection and differentiation of Blastocystis including its subtypes (arguably species) are essential to understand its epidemiology and role in human health. We compared (i) the sensitivity of conventional PCR (cPCR) and qPCR in a set of 288 DNA samples obtained from stool samples of gut-healthy individuals, and (ii) subtype diversity as detected by next-generation sequencing (NGS) versus Sanger sequencing. Real-time PCR resulted in more positive samples than cPCR, revealing high fecal load of Blastocystis based on the quantification curve in most samples. In subtype detection, NGS was largely in agreement with Sanger sequencing but showed higher sensitivity for mixed subtype colonization within one host. This fact together with use of the combination of qPCR and NGS and obtaining information on the fecal protist load will be beneficial for epidemiological and surveillance studies.

Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22019582
003      
CZ-PrNML
005      
20220804135806.0
007      
ta
008      
220720s2022 fr f 000 0|eng||
009      
AR
024    7_
$a 10.1051/parasite/2022029 $2 doi
035    __
$a (PubMed)35638752
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a fr
100    1_
$a Šloufová, Martina $u Institute of Parasitology, Biology Centre, the Czech Academy of Sciences, České Budějovice 370 05, Czech Republic - Department of Medical Biology, Faculty of Science, University of South Bohemia, České Budějovice 370 05, Czech Republic
245    10
$a Comparaison des approches de diagnostic moléculaire pour la détection et la différenciation du protiste intestinal Blastocystis sp. chez l'homme / $c M. Šloufová, Z. Lhotská, M. Jirků, KJ. Petrželková, CR. Stensvold, O. Cinek, KJ. Pomajbíková
246    31
$a Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans
520    9_
$a Blastocystis is the most commonly found intestinal protist in the world. Accurate detection and differentiation of Blastocystis including its subtypes (arguably species) are essential to understand its epidemiology and role in human health. We compared (i) the sensitivity of conventional PCR (cPCR) and qPCR in a set of 288 DNA samples obtained from stool samples of gut-healthy individuals, and (ii) subtype diversity as detected by next-generation sequencing (NGS) versus Sanger sequencing. Real-time PCR resulted in more positive samples than cPCR, revealing high fecal load of Blastocystis based on the quantification curve in most samples. In subtype detection, NGS was largely in agreement with Sanger sequencing but showed higher sensitivity for mixed subtype colonization within one host. This fact together with use of the combination of qPCR and NGS and obtaining information on the fecal protist load will be beneficial for epidemiological and surveillance studies.
650    12
$a Blastocystis $x genetika $7 D016844
650    12
$a blastocystóza $x diagnóza $x epidemiologie $7 D016776
650    _2
$a feces $7 D005243
650    _2
$a lidé $7 D006801
650    _2
$a molekulární patologie $7 D057089
650    _2
$a kvantitativní polymerázová řetězová reakce $7 D060888
655    _2
$a časopisecké články $7 D016428
700    1_
$a Lhotská, Zuzana $u Institute of Parasitology, Biology Centre, the Czech Academy of Sciences, České Budějovice 370 05, Czech Republic - Department of Medical Biology, Faculty of Science, University of South Bohemia, České Budějovice 370 05, Czech Republic
700    1_
$a Jirků, Milan $u Institute of Parasitology, Biology Centre, the Czech Academy of Sciences, České Budějovice 370 05, Czech Republic
700    1_
$a Petrželková, Klára J $u Institute of Parasitology, Biology Centre, the Czech Academy of Sciences, České Budějovice 370 05, Czech Republic - Institute of Vertebrate Biology, Czech Academy of Sciences, Květná 8, Brno 603 65, Czech Republic
700    1_
$a Stensvold, C Rune $u Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen DK-2300, Denmark
700    1_
$a Cinek, Ondřej $u Department of Pediatrics, 2nd Faculty of Medicine, Charles University and Motol University Hospital, Prague 150 06, Czech Republic
700    1_
$a Pomajbíková, Kateřina Jirků $u Institute of Parasitology, Biology Centre, the Czech Academy of Sciences, České Budějovice 370 05, Czech Republic - Department of Medical Biology, Faculty of Science, University of South Bohemia, České Budějovice 370 05, Czech Republic $1 https://orcid.org/0000000154116082
773    0_
$w MED00003686 $t Parasite (Paris, France) $x 1776-1042 $g Roč. 29, č. - (2022), s. 30
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35638752 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220720 $b ABA008
991    __
$a 20220804135800 $b ABA008
999    __
$a ok $b bmc $g 1822974 $s 1170825
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 29 $c - $d 30 $e 20220531 $i 1776-1042 $m Parasite $n Parasite $x MED00003686
GRA    __
$a RGY0078/2015 $p Human Frontier Science Program
GRA    __
$a 22-04837S $p Grantová Agentura České Republiky
GRA    __
$a 00064203 $p Ministerstvo Zdravotnictví Ceské Republiky
LZP    __
$a Pubmed-20220720

Find record

Citation metrics

    Archiving options