Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Toll-Like Receptor 3 Overexpression Induces Invasion of Prostate Cancer Cells, whereas Its Activation Triggers Apoptosis

XM. Muresan, E. Slabáková, J. Procházková, S. Drápela, R. Fedr, M. Pícková, O. Vacek, R. Víchová, T. Suchánková, J. Bouchal, D. Kürfürstová, M. Král, T. Hulínová, RP. Sýkora, V. Študent, V. Hejret, WM. van Weerden, M. Puhr, V. Pustka, D. Potěšil,...

. 2022 ; 192 (9) : 1321-1335. [pub] 20220622

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22024502

Toll-like receptor 3 (TLR3) is an endosomal receptor expressed in several immune and epithelial cells. Recent studies have highlighted its expression also in solid tumors, including prostate cancer (PCa), and have described its role primarily in the proinflammatory response and induction of apoptosis. It is up-regulated in some castration-resistant prostate cancers. However, the role of TLR3 in prostate cancer progression remains largely unknown. The current study experimentally demonstrated that exogenous TLR3 activation in PCa cell lines leads to a significant induction of secretion of the cytokines IL-6, IL-8, and interferon-β, depending on the model and chemoresistance status. Transcriptomic analysis of TLR3-overexpressing cells revealed a functional program that is enriched for genes involved in the regulation of cell motility, migration, and tumor invasiveness. Increased motility, migration, and invasion in TLR3-overexpressing cell line were confirmed by several in vitro assays and using an orthotopic prostate xenograft model in vivo. Furthermore, TLR3-ligand induced apoptosis via cleavage of caspase-3/7 and poly (ADP-ribose) polymerase, predominantly in TLR3-overexpressing cells. These results indicate that TLR3 may be involved in prostate cancer progression and metastasis; however, it might also represent an Achilles heel of PCa, which can be exploited for targeted therapy.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22024502
003      
CZ-PrNML
005      
20230119093447.0
007      
ta
008      
221017s2022 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.ajpath.2022.05.009 $2 doi
035    __
$a (PubMed)35750257
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Muresan, Ximena M $u Department of Cytokinetics, Institute of Biophysics of Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic
245    10
$a Toll-Like Receptor 3 Overexpression Induces Invasion of Prostate Cancer Cells, whereas Its Activation Triggers Apoptosis / $c XM. Muresan, E. Slabáková, J. Procházková, S. Drápela, R. Fedr, M. Pícková, O. Vacek, R. Víchová, T. Suchánková, J. Bouchal, D. Kürfürstová, M. Král, T. Hulínová, RP. Sýkora, V. Študent, V. Hejret, WM. van Weerden, M. Puhr, V. Pustka, D. Potěšil, Z. Zdráhal, Z. Culig, K. Souček
520    9_
$a Toll-like receptor 3 (TLR3) is an endosomal receptor expressed in several immune and epithelial cells. Recent studies have highlighted its expression also in solid tumors, including prostate cancer (PCa), and have described its role primarily in the proinflammatory response and induction of apoptosis. It is up-regulated in some castration-resistant prostate cancers. However, the role of TLR3 in prostate cancer progression remains largely unknown. The current study experimentally demonstrated that exogenous TLR3 activation in PCa cell lines leads to a significant induction of secretion of the cytokines IL-6, IL-8, and interferon-β, depending on the model and chemoresistance status. Transcriptomic analysis of TLR3-overexpressing cells revealed a functional program that is enriched for genes involved in the regulation of cell motility, migration, and tumor invasiveness. Increased motility, migration, and invasion in TLR3-overexpressing cell line were confirmed by several in vitro assays and using an orthotopic prostate xenograft model in vivo. Furthermore, TLR3-ligand induced apoptosis via cleavage of caspase-3/7 and poly (ADP-ribose) polymerase, predominantly in TLR3-overexpressing cells. These results indicate that TLR3 may be involved in prostate cancer progression and metastasis; however, it might also represent an Achilles heel of PCa, which can be exploited for targeted therapy.
650    _2
$a zvířata $7 D000818
650    _2
$a apoptóza $7 D017209
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a poly I-C $x farmakologie $7 D011070
650    _2
$a prostata $x patologie $7 D011467
650    12
$a nádory prostaty $x patologie $7 D011471
650    12
$a toll-like receptor 3 $x genetika $x metabolismus $7 D051196
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Slabáková, Eva $u Department of Cytokinetics, Institute of Biophysics of Czech Academy of Sciences, Brno, Czech Republic
700    1_
$a Procházková, Jiřina $u Department of Cytokinetics, Institute of Biophysics of Czech Academy of Sciences, Brno, Czech Republic
700    1_
$a Drápela, Stanislav $u Department of Cytokinetics, Institute of Biophysics of Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic $7 xx0280838
700    1_
$a Fedr, Radek $u Department of Cytokinetics, Institute of Biophysics of Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic
700    1_
$a Pícková, Markéta $u Department of Cytokinetics, Institute of Biophysics of Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
700    1_
$a Vacek, Ondřej $u Department of Cytokinetics, Institute of Biophysics of Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
700    1_
$a Víchová, Ráchel $u Department of Cytokinetics, Institute of Biophysics of Czech Academy of Sciences, Brno, Czech Republic
700    1_
$a Suchánková, Tereza $u Department of Cytokinetics, Institute of Biophysics of Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic
700    1_
$a Bouchal, Jan $u Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic
700    1_
$a Kürfürstová, Daniela $u Department of Urology, University Hospital, Olomouc, Czech Republic
700    1_
$a Král, Milan $u Department of Urology, University Hospital, Olomouc, Czech Republic
700    1_
$a Hulínová, Tereza $u Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic; Department of Clinical and Molecular Pathology, University Hospital, Ostrava, Czech Republic
700    1_
$a Sýkora, Radek P $u Department of Urology, University Hospital, Ostrava, Czech Republic
700    1_
$a Študent, Vladimír $u Department of Urology, University Hospital, Olomouc, Czech Republic
700    1_
$a Hejret, Václav $u Bioinformatics Core Facility Central European Institute of Technology, Masaryk University, Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic
700    1_
$a van Weerden, Wytske M $u Department of Urology, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, the Netherlands
700    1_
$a Puhr, Martin $u Proteomics Core Facility Central European Institute of Technology, Masaryk University, Brno, Czech Republic
700    1_
$a Pustka, Václav $u Department of Urology, Experimental Urology, Innsbruck Medical University, Innsbruck, Austria
700    1_
$a Potěšil, David $u Department of Urology, Experimental Urology, Innsbruck Medical University, Innsbruck, Austria
700    1_
$a Zdráhal, Zbyněk $u Department of Urology, Experimental Urology, Innsbruck Medical University, Innsbruck, Austria
700    1_
$a Culig, Zoran $u International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic; Proteomics Core Facility Central European Institute of Technology, Masaryk University, Brno, Czech Republic
700    1_
$a Souček, Karel $u Department of Cytokinetics, Institute of Biophysics of Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. Electronic address: ksoucek@ibp.cz
773    0_
$w MED00009121 $t American journal of pathology $x 1525-2191 $g Roč. 192, č. 9 (2022), s. 1321-1335
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35750257 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20221017 $b ABA008
991    __
$a 20230119093440 $b ABA008
999    __
$a ok $b bmc $g 1854297 $s 1175792
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 192 $c 9 $d 1321-1335 $e 20220622 $i 1525-2191 $m American journal of pathology $n Am J Pathol $x MED00009121
LZP    __
$a Pubmed-20221017

Najít záznam

Citační ukazatele

Možnosti archivace