• Something wrong with this record ?

Detection and molecular characterization of VRE isolates in Slovakia from stool samples positive for Clostridioides difficile toxins

J. Kuzma, L. Palcová, J. Timko, V. Bastová, V. Janošcová, D. Chmelař

. 2022 ; 67 (6) : 975-984. [pub] 20220823

Language English Country United States

Document type Journal Article

The study aimed to identify colonized patients as a possible source of eventual VRE (vancomycin-resistant enterococci) infection from stool samples positive for glutamate dehydrogenase antigen, as well as for Clostridioides difficile toxins A and B. The study was carried out from 7/2020 to 9/2021. Stool samples were grown in a brain heart infusion medium with a gram-positive non-spore-forming bacteria supplement under aerobic conditions. The samples for VRE identification were grown on CHROMID® VRE agar, and the MICs for vancomycin and teicoplanin were also estimated. The presence of the vanA/vanB genes was tested using the PCR method. The total number of 113 stool samples positive for Clostridioides difficile toxins was analyzed. Of these samples, 44 isolates with VRE characters were identified. The most prevalent isolates in our set of isolates were Enterococcus faecium (27 isolates, 62%), Enterococcus faecalis (9 isolates, 21%), Enterococcus solitarius (4 isolates, 9%), Enterococcus durans (2 isolates, 4%), 1 isolate Enterococcus sulfurous (2%), and Enterococcus raffinosus (2%). In total, 26 isolates were detected in the study in the presence of vanA genes (24 isolates E. faecium, 2 isolates E. faecalis) and 18 isolates detected in the presence of vanB genes (7 isolates E. faecalis, 4 isolates E. solitarius, 3 isolates E. faecium, 2 isolates E. durans, 1 isolate E. sulfurous, and E. raffinosus). The results of this study showed the local dominance character of the vanA gene of hospital VRE isolates that were carriers of genes associated with high resistance to vancomycin, teicoplanin, and occasionally linezolid.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22027718
003      
CZ-PrNML
005      
20240416153114.0
007      
ta
008      
221206s2022 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s12223-022-01002-2 $2 doi
035    __
$a (PubMed)35997873
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Kuzma, Jozef $u Faculty of Medicine, Institute of Laboratory Medicine, University of Ostrava, Ostrava, Czech Republic. jozef.kuzma@osu.cz $u Biomedical Research Center, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia. jozef.kuzma@osu.cz $1 https://orcid.org/0000000245138172
245    10
$a Detection and molecular characterization of VRE isolates in Slovakia from stool samples positive for Clostridioides difficile toxins / $c J. Kuzma, L. Palcová, J. Timko, V. Bastová, V. Janošcová, D. Chmelař
520    9_
$a The study aimed to identify colonized patients as a possible source of eventual VRE (vancomycin-resistant enterococci) infection from stool samples positive for glutamate dehydrogenase antigen, as well as for Clostridioides difficile toxins A and B. The study was carried out from 7/2020 to 9/2021. Stool samples were grown in a brain heart infusion medium with a gram-positive non-spore-forming bacteria supplement under aerobic conditions. The samples for VRE identification were grown on CHROMID® VRE agar, and the MICs for vancomycin and teicoplanin were also estimated. The presence of the vanA/vanB genes was tested using the PCR method. The total number of 113 stool samples positive for Clostridioides difficile toxins was analyzed. Of these samples, 44 isolates with VRE characters were identified. The most prevalent isolates in our set of isolates were Enterococcus faecium (27 isolates, 62%), Enterococcus faecalis (9 isolates, 21%), Enterococcus solitarius (4 isolates, 9%), Enterococcus durans (2 isolates, 4%), 1 isolate Enterococcus sulfurous (2%), and Enterococcus raffinosus (2%). In total, 26 isolates were detected in the study in the presence of vanA genes (24 isolates E. faecium, 2 isolates E. faecalis) and 18 isolates detected in the presence of vanB genes (7 isolates E. faecalis, 4 isolates E. solitarius, 3 isolates E. faecium, 2 isolates E. durans, 1 isolate E. sulfurous, and E. raffinosus). The results of this study showed the local dominance character of the vanA gene of hospital VRE isolates that were carriers of genes associated with high resistance to vancomycin, teicoplanin, and occasionally linezolid.
650    _2
$a lidé $7 D006801
650    12
$a enterokoky rezistentní vůči vankomycinu $x genetika $7 D065507
650    _2
$a vankomycin $x farmakologie $7 D014640
650    12
$a Clostridioides difficile $x genetika $7 D016360
650    _2
$a teikoplanin $x farmakologie $7 D017334
650    12
$a grampozitivní bakteriální infekce $x mikrobiologie $7 D016908
650    12
$a Enterococcus faecium $x genetika $7 D016984
650    _2
$a mikrobiální testy citlivosti $7 D008826
650    _2
$a antibakteriální látky $x farmakologie $7 D000900
650    _2
$a bakteriální proteiny $x genetika $7 D001426
651    _2
$a Slovenská republika $7 D018154
655    _2
$a časopisecké články $7 D016428
700    1_
$a Palcová, Lenka $u Science and Research Department, Faculty Hospital, Central Military Hospital, Ružomberok, Slovakia $u St. Elizabeth University in Bratislava, Catholic University, Ružomberok, Slovakia
700    1_
$a Timko, Jaroslav $u Laboratory of Clinical Microbiology, Faculty Hospital, Central Military Hospital, Ružomberok, Slovakia $u Catholic University, Ružomberok, Slovakia
700    1_
$a Bastová, Veronika $u Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic
700    1_
$a Janošcová, Veronika $u Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic
700    1_
$a Chmelař, Dittmar $u Czech Anaerobic Bacteria Reference Laboratory, Faculty of Medicine, Institute of Laboratory Medicine, University of Ostrava, Ostrava, Czech Republic
773    0_
$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 67, č. 6 (2022), s. 975-984
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35997873 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20221206 $b ABA008
991    __
$a 20240416153111 $b ABA008
999    __
$a ok $b bmc $g 1867313 $s 1179031
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2022 $b 67 $c 6 $d 975-984 $e 20220823 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
LZP    __
$a Pubmed-20221206

Find record

Citation metrics

Loading data ...

Archiving options

Loading data ...