Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Intestinal parasites of buffalo calves from Romania: molecular characterisation of Cryptosporidium spp. and Giardia duodenalis, and the first report of Eimeria bareillyi

DA. Barburas, V. Cozma, AM. Ionica, I. Abbas, R. Barburas, V. Mircean, G. D'Amico, JP. Dubey, A. Gyorke

. 2022 ; 69 (-) : . [pub] 20220808

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22029593

Buffaloes represent an important economic resource for several regions of the world including Romania. In the present study, we examined 104 faecal samples collected from 38 buffalo calves (2-11 weeks old) from household rearing systems in Romania for gastrointestinal parasites. All samples were tested using the saturated salt flotation, McMaster and modified Ziehl-Nielsen staining methods. PCR coupled with sequencing isolates were used to identify assemblages of Giardia lamblia (Kunstler, 1882) and species of Cryptosporidium Tyzzer, 1907. Overall, 33 out of 38 examined buffalo calves were infected with different gastrointestinal parasites: 16 had single infections and 17 had mixed infections with two or three parasites. Species of Eimeria Schneider, 1875 (32/38; 84%) were the most prevalent parasites; eight species were identified according to the oocyst morphology, including the pathogenic E. bareillyi (Gill, Chhabra et Lall, 1963) which was detected for the first time in buffaloes from Romania. The nematodes Toxocara vitulorum (Goeze, 1782) (11/38; 37%) and Strongyloides papillosus (Wedl, 1856) (6/38; 16%) were also detected. Cryptosporidium spp. were found in four (11%) buffalo calves; two of them were molecularly identified as C. ryanae Fayer, Santin et Trout, 2008, and another one clustered in the same clade with C. ryanae, C. bovis Fayer, Santin et Xiao, 2005, and C. xiaoi Fayer et Santin, 2009. Giardia duodenalis assemblage E was also molecularly detected in a single (2.6%) buffalo calf. The presence of other buffaloes in the same barn was identified as a risk factor for infection with T. vitulorum. Our results indicate extensive parasitic infections in buffalo calves from northwestern Romania and underline the necessity of prophylactic treatments for T. vitulorum and E. bareillyi.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22029593
003      
CZ-PrNML
005      
20230208112532.0
007      
ta
008      
230113s2022 xr f 000 0|eng||
009      
AR
024    7_
$a 10.14411/fp.2022.015 $2 doi
035    __
$a (PubMed)35993791
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Barburas, Diana Ancuta $u Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania $u These authors contributed equally to the work $u Corresponding author
245    10
$a Intestinal parasites of buffalo calves from Romania: molecular characterisation of Cryptosporidium spp. and Giardia duodenalis, and the first report of Eimeria bareillyi / $c DA. Barburas, V. Cozma, AM. Ionica, I. Abbas, R. Barburas, V. Mircean, G. D'Amico, JP. Dubey, A. Gyorke
520    9_
$a Buffaloes represent an important economic resource for several regions of the world including Romania. In the present study, we examined 104 faecal samples collected from 38 buffalo calves (2-11 weeks old) from household rearing systems in Romania for gastrointestinal parasites. All samples were tested using the saturated salt flotation, McMaster and modified Ziehl-Nielsen staining methods. PCR coupled with sequencing isolates were used to identify assemblages of Giardia lamblia (Kunstler, 1882) and species of Cryptosporidium Tyzzer, 1907. Overall, 33 out of 38 examined buffalo calves were infected with different gastrointestinal parasites: 16 had single infections and 17 had mixed infections with two or three parasites. Species of Eimeria Schneider, 1875 (32/38; 84%) were the most prevalent parasites; eight species were identified according to the oocyst morphology, including the pathogenic E. bareillyi (Gill, Chhabra et Lall, 1963) which was detected for the first time in buffaloes from Romania. The nematodes Toxocara vitulorum (Goeze, 1782) (11/38; 37%) and Strongyloides papillosus (Wedl, 1856) (6/38; 16%) were also detected. Cryptosporidium spp. were found in four (11%) buffalo calves; two of them were molecularly identified as C. ryanae Fayer, Santin et Trout, 2008, and another one clustered in the same clade with C. ryanae, C. bovis Fayer, Santin et Xiao, 2005, and C. xiaoi Fayer et Santin, 2009. Giardia duodenalis assemblage E was also molecularly detected in a single (2.6%) buffalo calf. The presence of other buffaloes in the same barn was identified as a risk factor for infection with T. vitulorum. Our results indicate extensive parasitic infections in buffalo calves from northwestern Romania and underline the necessity of prophylactic treatments for T. vitulorum and E. bareillyi.
650    _2
$a zvířata $7 D000818
650    _2
$a buvoli $x parazitologie $7 D002020
650    12
$a kryptosporidióza $x epidemiologie $x parazitologie $7 D003457
650    12
$a Cryptosporidium $7 D003458
650    12
$a Eimeria $7 D004539
650    _2
$a feces $x parazitologie $7 D005243
650    12
$a Giardia lamblia $x genetika $7 D016829
650    12
$a paraziti $7 D010271
651    _2
$a Rumunsko $x epidemiologie $7 D012383
655    _2
$a časopisecké články $7 D016428
700    1_
$a Cozma, Vasile $u Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania $u These authors contributed equally to the work
700    1_
$a Ionica, Angela Monica $u Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania $u CDS-9, Life Science Institute, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania $u These authors contributed equally to the work
700    1_
$a Abbas, Ibrahim $u Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt $u These authors contributed equally to the work
700    1_
$a Barburas, Remus $u S.C. Medivet Barburas S.R.L, Romanasi, Salaj, Romania
700    1_
$a Mircean, Viorica $u Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania
700    1_
$a D'Amico, Gianluca $u Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania
700    1_
$a Dubey, Jitender P $u United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Disease Laboratory, Beltsville, USA
700    1_
$a Gyorke, Adriana $u Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania $u These authors contributed equally to the work $u Corresponding author
773    0_
$w MED00011006 $t Folia parasitologica $x 1803-6465 $g Roč. 69, č. - (2022)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35993791 $y Pubmed
910    __
$a ABA008 $b online $c $y - $z 0
990    __
$a 20230113 $b ABA008
991    __
$a 20230208112526 $b ABA008
999    __
$a kom $b bmc $g 1895207 $s 1180918
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2022 $b 69 $c - $e 20220808 $i 1803-6465 $m Folia parasitologica $n Folia parasitol. $x MED00011006
LZP    __
$a Pubmed-20230113

Najít záznam

Citační ukazatele

Možnosti archivace