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Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor

I. Palacio, M. Moreno, A. Náñez, A. Purwidyantri, T. Domingues, PD. Cabral, J. Borme, M. Marciello, JI. Mendieta-Moreno, B. Torres-Vázquez, JI. Martínez, MF. López, M. García-Hernández, L. Vázquez, P. Jelínek, P. Alpuim, C. Briones, JÁ. Martín-Gago

. 2023 ; 222 (-) : 115006. [pub] 20221210

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22031950

Biosensors based on graphene field-effect transistors have become a promising tool for detecting a broad range of analytes. However, their performance is substantially affected by the functionalization protocol. In this work, we use a controlled in-vacuum physical method for the covalent functionalization of graphene to construct ultrasensitive aptamer-based biosensors (aptasensors) able to detect hepatitis C virus core protein. These devices are highly specific and robust, achieving attomolar detection of the viral protein in human blood plasma. Such an improved sensitivity is rationalized by theoretical calculations showing that induced polarization at the graphene interface, caused by the proximity of covalently bound molecular probe, modulates the charge balance at the graphene/aptamer interface. This charge balance causes a net shift of the Dirac cone providing enhanced sensitivity for the attomolar detection of the target proteins. Such an unexpected effect paves the way for using this kind of graphene-based functionalized platforms for ultrasensitive and real-time diagnostics of different diseases.

Citace poskytuje Crossref.org

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$a Palacio, Irene $u Institute of Material Science of Madrid (ICMM-CSIC), C/Sor Juana Inés de La Cruz 3, 28049, Madrid, Spain. Electronic address: i.palacio@csic.es
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$a Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor / $c I. Palacio, M. Moreno, A. Náñez, A. Purwidyantri, T. Domingues, PD. Cabral, J. Borme, M. Marciello, JI. Mendieta-Moreno, B. Torres-Vázquez, JI. Martínez, MF. López, M. García-Hernández, L. Vázquez, P. Jelínek, P. Alpuim, C. Briones, JÁ. Martín-Gago
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$a Moreno, Miguel $u Centro de Astrobiología (CAB, INTA-CSIC), 28850, Torrejón de Ardoz, Madrid, Spain
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$a Náñez, Almudena $u Centro de Astrobiología (CAB, INTA-CSIC), 28850, Torrejón de Ardoz, Madrid, Spain
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$a Borme, Jérôme $u International Iberian Nanotechnology Laboratory (INL), 4715-330, Braga, Portugal
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$a Marciello, Marzia $u Department of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, Complutense University (UCM), Plaza Ramón y Cajal, 28040, Madrid, Spain
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$a Mendieta-Moreno, Jesús Ignacio $u Institute of Physics, Czech Academy of Sciences, 16200, Prague, Czech Republic
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$a Torres-Vázquez, Beatriz $u Centro de Astrobiología (CAB, INTA-CSIC), 28850, Torrejón de Ardoz, Madrid, Spain; Universidad de Alcalá, Facultad de Medicina, Madrid, Spain
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$a López, María Francisca $u Institute of Material Science of Madrid (ICMM-CSIC), C/Sor Juana Inés de La Cruz 3, 28049, Madrid, Spain
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$a García-Hernández, Mar $u Institute of Material Science of Madrid (ICMM-CSIC), C/Sor Juana Inés de La Cruz 3, 28049, Madrid, Spain
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$a Alpuim, Pedro $u International Iberian Nanotechnology Laboratory (INL), 4715-330, Braga, Portugal; Centro de Física das Universidades do Minho e Porto (CF-UM-UP), Universidade do Minho, 4710-057, Braga, Portugal. Electronic address: pedro.alpuim.us@inl.int
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$a Briones, Carlos $u Centro de Astrobiología (CAB, INTA-CSIC), 28850, Torrejón de Ardoz, Madrid, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain. Electronic address: cbriones@cab.inta-csic.es
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$a Martín-Gago, José Ángel $u Institute of Material Science of Madrid (ICMM-CSIC), C/Sor Juana Inés de La Cruz 3, 28049, Madrid, Spain. Electronic address: gago@icmm.csic.es
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