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Preparative protein concentration from biofluids by epitachophoresis
H. Hrušková, I. Voráčová, M. Laštovičková, M. Killinger, F. Foret
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- cytochromy c MeSH
- HeLa buňky MeSH
- izotachoforéza * metody MeSH
- lidé MeSH
- proteiny MeSH
- tělesné tekutiny * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
With increasing demands on protein analyses in complex biological matrices, the insistence on developing new sample preparation techniques is rising. Recently, we introduced a new displacement electrophoresis technique (epitachophoresis) and instrumentation for preparative concentration and cleaning of DNA samples. This work describes the possibility of applying this device to protein samples. We have developed a method for the epitachophoretic concentration of proteins in a cationic mode and tested it by concentrating and collecting the protein zones from complex biological matrices (urine and growth medium). Under optimized conditions, we have obtained recoveries up to 99%. Furthermore, the applicability of the developed method was proven by concentrating and collecting the cytochrome c zone from a HeLa cell line growth medium, where the protein cytochrome c was released during cell apoptosis.
Czech Academy of Sciences Institute of Analytical Chemistry Veveří 967 97 Brno 602 00 Czech Republic
Department of Chemistry Faculty of Science Masaryk University Kamenice 5 Brno 625 00 Czech Republic
Citace poskytuje Crossref.org
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