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Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
E. Makhneva, D. Sklenárová, JC. Brandmeier, A. Hlaváček, HH. Gorris, P. Skládal, Z. Farka
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Immunoassay methods MeSH
- Immunosorbents * MeSH
- Humans MeSH
- Limit of Detection MeSH
- Magnetics MeSH
- Nanoparticles * MeSH
- Streptavidin MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
References provided by Crossref.org
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