Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

DNA hypermethylation of CADM1, PAX5, WT1, RARβ, and PAX6 genes in oropharyngeal cancer associated with human papillomavirus

N. Birknerová, H. Kovaříková, I. Baranová, A. Přikrylová, J. Laco, H. Vošmiková, B. Gajdošová, M. Hodek, M. Vošmik, V. Palička, M. Chmelařová

. 2022 ; 17 (11) : 1301-1310. [pub] 20220103

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22032941

Recently, an increasing incidence of HPV-induced oropharyngeal squamous cell carcinoma (OPSCC) has been observed. Moreover, locoregionally advanced stages require a combined modal approach, and the prognosis is poor. Therefore, it is essential to find early diagnostic and prognostic biomarkers. DNA methylation changes play a crucial role in the process of carcinogenesis and are often investigated as promising biomarkers in many types of cancer. For analysis of DNA methylation levels of selected tumour suppressor genes in HPV-positive and HPV-negative samples (including primary tumours and corresponding metastases of metastasizing OPSCCs, primary tumours of non-metastasizing OPSCCs, and control samples), methylation-specific MLPA and methylation-specific high-resolution melting analyses were used. A significant difference in methylation between OPSCCs and the control group was observed in WT1, PAX6 (P < 0.01) and CADM1, RARβ (P < 0.05) genes. CADM1 and WT1 hypermethylation was detected mostly in HPV-positive samples; all but one HPV-negative samples were unmethylated. Moreover, hypermethylation of PAX5 gene was observed in metastases compared with control samples and was also associated with shorter overall survival of all patients (P < 0.05). Associations described herein between promoter methylation of selected genes and clinicopathological data could benefit OPSCC patients in the future by improvement in screening, early detection, and prognosis of the disease.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22032941
003      
CZ-PrNML
005      
20230131151303.0
007      
ta
008      
230120s2022 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1080/15592294.2021.2018812 $2 doi
035    __
$a (PubMed)34974810
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Birknerová, Natália $u Department of Clinical Biochemistry and Diagnostics, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $1 https://orcid.org/0000000220066694
245    10
$a DNA hypermethylation of CADM1, PAX5, WT1, RARβ, and PAX6 genes in oropharyngeal cancer associated with human papillomavirus / $c N. Birknerová, H. Kovaříková, I. Baranová, A. Přikrylová, J. Laco, H. Vošmiková, B. Gajdošová, M. Hodek, M. Vošmik, V. Palička, M. Chmelařová
520    9_
$a Recently, an increasing incidence of HPV-induced oropharyngeal squamous cell carcinoma (OPSCC) has been observed. Moreover, locoregionally advanced stages require a combined modal approach, and the prognosis is poor. Therefore, it is essential to find early diagnostic and prognostic biomarkers. DNA methylation changes play a crucial role in the process of carcinogenesis and are often investigated as promising biomarkers in many types of cancer. For analysis of DNA methylation levels of selected tumour suppressor genes in HPV-positive and HPV-negative samples (including primary tumours and corresponding metastases of metastasizing OPSCCs, primary tumours of non-metastasizing OPSCCs, and control samples), methylation-specific MLPA and methylation-specific high-resolution melting analyses were used. A significant difference in methylation between OPSCCs and the control group was observed in WT1, PAX6 (P < 0.01) and CADM1, RARβ (P < 0.05) genes. CADM1 and WT1 hypermethylation was detected mostly in HPV-positive samples; all but one HPV-negative samples were unmethylated. Moreover, hypermethylation of PAX5 gene was observed in metastases compared with control samples and was also associated with shorter overall survival of all patients (P < 0.05). Associations described herein between promoter methylation of selected genes and clinicopathological data could benefit OPSCC patients in the future by improvement in screening, early detection, and prognosis of the disease.
650    _2
$a lidé $7 D006801
650    12
$a Alphapapillomavirus $7 D052685
650    _2
$a buněčná adhezní molekula 1 $x genetika $x metabolismus $7 D000075982
650    _2
$a DNA $x metabolismus $7 D004247
650    _2
$a metylace DNA $7 D019175
650    12
$a nádory hlavy a krku $x genetika $7 D006258
650    12
$a nádory orofaryngu $x patologie $7 D009959
650    _2
$a Papillomaviridae $7 D027383
650    12
$a infekce papilomavirem $x komplikace $7 D030361
650    _2
$a aktivátorový protein specifický pro B-buňky $x genetika $7 D051757
650    _2
$a prognóza $7 D011379
650    _2
$a dlaždicobuněčné karcinomy hlavy a krku $x genetika $7 D000077195
650    _2
$a proteiny WT1 $x genetika $x metabolismus $7 D025721
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Kovaříková, Helena $u Department of Clinical Biochemistry and Diagnostics, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $1 https://orcid.org/0000000150404140
700    1_
$a Baranová, Ivana $u Department of Clinical Biochemistry and Diagnostics, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $1 https://orcid.org/0000000182732595
700    1_
$a Přikrylová, Albína $u Department of Clinical Biochemistry and Diagnostics, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic
700    1_
$a Laco, Jan $u The Fingerland Department of Pathology, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $1 https://orcid.org/0000000296027501
700    1_
$a Vošmiková, Hana $u The Fingerland Department of Pathology, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $1 https://orcid.org/0000000185953991
700    1_
$a Gajdošová, Barbora $u The Fingerland Department of Pathology, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic
700    1_
$a Hodek, Miroslav $u Department of Oncology and Radiotherapy, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $1 https://orcid.org/0000000245202824
700    1_
$a Vošmik, Milan $u Department of Oncology and Radiotherapy, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $1 https://orcid.org/0000000164977505
700    1_
$a Palička, Vladimír $u Department of Clinical Biochemistry and Diagnostics, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic $1 https://orcid.org/0000000172361647
700    1_
$a Chmelařová, Marcela $u Department of Clinical Biochemistry and Diagnostics, Charles University, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic
773    0_
$w MED00180218 $t Epigenetics $x 1559-2308 $g Roč. 17, č. 11 (2022), s. 1301-1310
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34974810 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230120 $b ABA008
991    __
$a 20230131151259 $b ABA008
999    __
$a ok $b bmc $g 1891607 $s 1184276
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2022 $b 17 $c 11 $d 1301-1310 $e 20220103 $i 1559-2308 $m Epigenetics. $n Epigenetics $x MED00180218
LZP    __
$a Pubmed-20230120

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace