Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Gut microbiome diversity of porcine peritonitis model of sepsis

M. Chalupova, J. Horak, L. Kramna, L. Nalos, M. Stengl, K. Chudejova, L. Kraftova, O. Cinek, P. Klein, M. Matejovic, J. Hrabak

. 2022 ; 12 (1) : 17430. [pub] 20221019

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22033028

Animal models are essential in understanding of the mechanisms of sepsis moreover the development and the assessment of emerging therapies. In clinically relevant porcine model, however, a significant variability in the host response has been observed among animals. Thus, there is a strong demand to better understand the potential sources of this heterogeneity. In this study, we compared faecal microbiome composition of 12 animals. Three samples were collected at different time points from each animal. Bacteriome was subjected to 16S rDNA profiling. A significant difference in bacterial composition was associated with the season (p < 0.001) but not with the sex of the pig (p = 0.28), the timing of sample collection (p = 0.59), or interactions thereof (all p > 0.3). The season batch explained 55% of the total variance in the bacteriome diversity. The season term was highly significant from the high-resolution level of the bacterial amplicon sequencing variants up to the level of phylum. The diversity of the microbiome composition could significantly influence experimental model of sepsis, and studies are warranted to demonstrate the effects of gut microbiome diversity on the host-response. If confirmed, control of the gut microbiome should become a standard part of the pre-clinical sepsis experiments.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22033028
003      
CZ-PrNML
005      
20230131151730.0
007      
ta
008      
230120s2022 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-022-21079-6 $2 doi
035    __
$a (PubMed)36261543
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Chalupova, Miroslava $u Biomedical Center, Faculty Medicine in Pilsen, Charles University, alej. Svobody 76, 323 00, Pilsen, Czech Republic $u Department of Stomatology, Faculty Medicine in Pilsen, Charles University, Pilsen, Czech Republic
245    10
$a Gut microbiome diversity of porcine peritonitis model of sepsis / $c M. Chalupova, J. Horak, L. Kramna, L. Nalos, M. Stengl, K. Chudejova, L. Kraftova, O. Cinek, P. Klein, M. Matejovic, J. Hrabak
520    9_
$a Animal models are essential in understanding of the mechanisms of sepsis moreover the development and the assessment of emerging therapies. In clinically relevant porcine model, however, a significant variability in the host response has been observed among animals. Thus, there is a strong demand to better understand the potential sources of this heterogeneity. In this study, we compared faecal microbiome composition of 12 animals. Three samples were collected at different time points from each animal. Bacteriome was subjected to 16S rDNA profiling. A significant difference in bacterial composition was associated with the season (p < 0.001) but not with the sex of the pig (p = 0.28), the timing of sample collection (p = 0.59), or interactions thereof (all p > 0.3). The season batch explained 55% of the total variance in the bacteriome diversity. The season term was highly significant from the high-resolution level of the bacterial amplicon sequencing variants up to the level of phylum. The diversity of the microbiome composition could significantly influence experimental model of sepsis, and studies are warranted to demonstrate the effects of gut microbiome diversity on the host-response. If confirmed, control of the gut microbiome should become a standard part of the pre-clinical sepsis experiments.
650    _2
$a prasata $7 D013552
650    _2
$a zvířata $7 D000818
650    12
$a střevní mikroflóra $x genetika $7 D000069196
650    _2
$a RNA ribozomální 16S $x genetika $7 D012336
650    _2
$a Bacteria $x genetika $7 D001419
650    _2
$a ribozomální DNA $x genetika $7 D004275
650    12
$a sepse $7 D018805
650    12
$a peritonitida $7 D010538
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Horak, Jan $u Biomedical Center, Faculty Medicine in Pilsen, Charles University, alej. Svobody 76, 323 00, Pilsen, Czech Republic $u Department of Internal Medicine, Faculty Medicine in Pilsen, Charles University, Pilsen, Czech Republic
700    1_
$a Kramna, Lenka $u Department of Paediatrics, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic
700    1_
$a Nalos, Lukas $u Biomedical Center, Faculty Medicine in Pilsen, Charles University, alej. Svobody 76, 323 00, Pilsen, Czech Republic $u Department of Physiology, Faculty Medicine in Pilsen, Charles University, Pilsen, Czech Republic
700    1_
$a Stengl, Milan $u Biomedical Center, Faculty Medicine in Pilsen, Charles University, alej. Svobody 76, 323 00, Pilsen, Czech Republic $u Department of Physiology, Faculty Medicine in Pilsen, Charles University, Pilsen, Czech Republic
700    1_
$a Chudejova, Katerina $u Biomedical Center, Faculty Medicine in Pilsen, Charles University, alej. Svobody 76, 323 00, Pilsen, Czech Republic $u Department of Microbiology, Faculty Medicine in Pilsen, Charles University, Pilsen, Czech Republic
700    1_
$a Kraftova, Lucie $u Biomedical Center, Faculty Medicine in Pilsen, Charles University, alej. Svobody 76, 323 00, Pilsen, Czech Republic $u Department of Microbiology, Faculty Medicine in Pilsen, Charles University, Pilsen, Czech Republic
700    1_
$a Cinek, Ondrej $u Department of Paediatrics, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic
700    1_
$a Klein, Pavel $u Biomedical Center, Faculty Medicine in Pilsen, Charles University, alej. Svobody 76, 323 00, Pilsen, Czech Republic
700    1_
$a Matejovic, Martin $u Biomedical Center, Faculty Medicine in Pilsen, Charles University, alej. Svobody 76, 323 00, Pilsen, Czech Republic $u Department of Internal Medicine, Faculty Medicine in Pilsen, Charles University, Pilsen, Czech Republic
700    1_
$a Hrabak, Jaroslav $u Biomedical Center, Faculty Medicine in Pilsen, Charles University, alej. Svobody 76, 323 00, Pilsen, Czech Republic. Jaroslav.Hrabak@lfp.cuni.cz $u Department of Microbiology, Faculty Medicine in Pilsen, Charles University, Pilsen, Czech Republic. Jaroslav.Hrabak@lfp.cuni.cz
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 12, č. 1 (2022), s. 17430
856    41
$u https://pubmed.ncbi.nlm.nih.gov/36261543 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230120 $b ABA008
991    __
$a 20230131151725 $b ABA008
999    __
$a ok $b bmc $g 1891662 $s 1184363
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2022 $b 12 $c 1 $d 17430 $e 20221019 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
LZP    __
$a Pubmed-20230120

Najít záznam

Citační ukazatele

Možnosti archivace