• Something wrong with this record ?

Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration

V. Fedorova, V. Pospisilova, T. Vanova, K. Amruz Cerna, P. Abaffy, J. Sedmik, J. Raska, S. Vochyanova, Z. Matusova, J. Houserova, L. Valihrach, Z. Hodny, D. Bohaciakova

. 2023 ; 17 (4) : 647-663. [pub] 20230218

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc23003668

It is currently challenging to adequately model the growth and migration of glioblastoma using two-dimensional (2D) in vitro culture systems as they quickly lose the original, patient-specific identity and heterogeneity. However, with the advent of three-dimensional (3D) cell cultures and human-induced pluripotent stem cell (iPSC)-derived cerebral organoids (COs), studies demonstrate that the glioblastoma-CO (GLICO) coculture model helps to preserve the phenotype of the patient-specific tissue. Here, we aimed to set up such a model using mature COs and develop a pipeline for subsequent analysis of cocultured glioblastoma. Our data demonstrate that the growth and migration of the glioblastoma cell line within the mature COs are significantly increased in the presence of extracellular matrix proteins, shortening the time needed for glioblastoma to initiate migration. We also describe in detail the method for the visualization and quantification of these migrating cells within the GLICO model. Lastly, we show that this coculture model (and the human brain-like microenvironment) can significantly transform the gene expression profile of the established U87 glioblastoma cell line into proneural and classical glioblastoma cell types.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23003668
003      
CZ-PrNML
005      
20230425140805.0
007      
ta
008      
230418s2023 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1002/1878-0261.13389 $2 doi
035    __
$a (PubMed)36744875
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Fedorova, Veronika $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
245    10
$a Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration / $c V. Fedorova, V. Pospisilova, T. Vanova, K. Amruz Cerna, P. Abaffy, J. Sedmik, J. Raska, S. Vochyanova, Z. Matusova, J. Houserova, L. Valihrach, Z. Hodny, D. Bohaciakova
520    9_
$a It is currently challenging to adequately model the growth and migration of glioblastoma using two-dimensional (2D) in vitro culture systems as they quickly lose the original, patient-specific identity and heterogeneity. However, with the advent of three-dimensional (3D) cell cultures and human-induced pluripotent stem cell (iPSC)-derived cerebral organoids (COs), studies demonstrate that the glioblastoma-CO (GLICO) coculture model helps to preserve the phenotype of the patient-specific tissue. Here, we aimed to set up such a model using mature COs and develop a pipeline for subsequent analysis of cocultured glioblastoma. Our data demonstrate that the growth and migration of the glioblastoma cell line within the mature COs are significantly increased in the presence of extracellular matrix proteins, shortening the time needed for glioblastoma to initiate migration. We also describe in detail the method for the visualization and quantification of these migrating cells within the GLICO model. Lastly, we show that this coculture model (and the human brain-like microenvironment) can significantly transform the gene expression profile of the established U87 glioblastoma cell line into proneural and classical glioblastoma cell types.
650    _2
$a lidé $7 D006801
650    12
$a glioblastom $x genetika $x metabolismus $7 D005909
650    _2
$a organoidy $x metabolismus $7 D009940
650    _2
$a mozek $7 D001921
650    _2
$a buněčné linie $7 D002460
650    _2
$a buněčné kultury $x metody $7 D018929
650    _2
$a nádorové mikroprostředí $7 D059016
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Pospisilova, Veronika $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
700    1_
$a Vanova, Tereza $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic $u International Clinical Research Center (ICRC), St. Anne's University Hospital, Brno, Czech Republic
700    1_
$a Amruz Cerna, Katerina $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
700    1_
$a Abaffy, Pavel $u Laboratory of Gene Expression, Institute of Biotechnology CAS, BIOCEV, Vestec, Czech Republic $1 https://orcid.org/0000000275718880
700    1_
$a Sedmik, Jiri $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
700    1_
$a Raska, Jan $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
700    1_
$a Vochyanova, Simona $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
700    1_
$a Matusova, Zuzana $u Laboratory of Gene Expression, Institute of Biotechnology CAS, BIOCEV, Vestec, Czech Republic
700    1_
$a Houserova, Jana $u Department of Genome Integrity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Valihrach, Lukas $u Laboratory of Gene Expression, Institute of Biotechnology CAS, BIOCEV, Vestec, Czech Republic
700    1_
$a Hodny, Zdenek $u Department of Genome Integrity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Bohaciakova, Dasa $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic $u International Clinical Research Center (ICRC), St. Anne's University Hospital, Brno, Czech Republic $1 https://orcid.org/0000000295386668 $7 mub20221157350
773    0_
$w MED00167281 $t Molecular oncology $x 1878-0261 $g Roč. 17, č. 4 (2023), s. 647-663
856    41
$u https://pubmed.ncbi.nlm.nih.gov/36744875 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230418 $b ABA008
991    __
$a 20230425140801 $b ABA008
999    __
$a ok $b bmc $g 1924379 $s 1189877
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 17 $c 4 $d 647-663 $e 20230218 $i 1878-0261 $m Molecular oncology $n Mol Oncol $x MED00167281
LZP    __
$a Pubmed-20230418
Back

Find record

Citation metrics

Archiving options