Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
21-21510S
Grantová Agentura České Republiky
20-15728S
Grantová Agentura České Republiky
18-25429Y
Grantová Agentura České Republiky
NV19-08-00472
AZV
CEP - Centrální evidence projektů
NU21-08-00373
AZV
CEP - Centrální evidence projektů
MUNI/11/SUP/19/2020
Grant Agency of Masaryk University
MUNI/11/SUP/01/2022
Grant Agency of Masaryk University
MUNI/A/1398/2021
Grant Agency of Masaryk University
CZ.02.1.01/0.0/0.0/16_026/0008451
European Regional Development Fund - Project INBIO
86652036
RVO
CEP - Centrální evidence projektů
MUNI/R/1321/2021
Career Restart Grant
MUNI/R/1697/2020
Career Restart Grant
ROZV/28/LF22/2020
Medical Faculty MU
ROZV/23/LF19/2019
Medical Faculty MU
MUNI/IGA/1273/2021
JCMM - PhD Talent
LQ1605
MEYS CR
CZ.02.1.01/0.0/0.0/16_013/0001761
MEYS CR
LM2018121
MEYS CR
LM2018132
MEYS CR
LM2018129
MEYS CR
857560
European Union's Horizon 2020 Research and Innovation Program
CZ.02.1.01/0.0/0.0/17_043/0009632
Operational Programme Research, Development and Innovation - project CETOCOEN EXCELLENCE
PubMed
36744875
PubMed Central
PMC10061278
DOI
10.1002/1878-0261.13389
Knihovny.cz E-zdroje
- Klíčová slova
- GLICO, cerebral organoids, glioblastoma, induced pluripotent stem cells,
- MeSH
- buněčné kultury metody MeSH
- buněčné linie MeSH
- glioblastom * genetika metabolismus MeSH
- lidé MeSH
- mozek MeSH
- nádorové mikroprostředí MeSH
- organoidy metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
It is currently challenging to adequately model the growth and migration of glioblastoma using two-dimensional (2D) in vitro culture systems as they quickly lose the original, patient-specific identity and heterogeneity. However, with the advent of three-dimensional (3D) cell cultures and human-induced pluripotent stem cell (iPSC)-derived cerebral organoids (COs), studies demonstrate that the glioblastoma-CO (GLICO) coculture model helps to preserve the phenotype of the patient-specific tissue. Here, we aimed to set up such a model using mature COs and develop a pipeline for subsequent analysis of cocultured glioblastoma. Our data demonstrate that the growth and migration of the glioblastoma cell line within the mature COs are significantly increased in the presence of extracellular matrix proteins, shortening the time needed for glioblastoma to initiate migration. We also describe in detail the method for the visualization and quantification of these migrating cells within the GLICO model. Lastly, we show that this coculture model (and the human brain-like microenvironment) can significantly transform the gene expression profile of the established U87 glioblastoma cell line into proneural and classical glioblastoma cell types.
Department of Histology and Embryology Faculty of Medicine Masaryk University Brno Czech Republic
International Clinical Research Center St Anne's University Hospital Brno Czech Republic
Laboratory of Gene Expression Institute of Biotechnology CAS BIOCEV Vestec Czech Republic
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