Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
34088007
DOI
10.1016/j.scr.2021.102378
PII: S1873-5061(21)00224-5
Knihovny.cz E-zdroje
- MeSH
- Alzheimerova nemoc * MeSH
- buněčná diferenciace MeSH
- indukované pluripotentní kmenové buňky * MeSH
- leukocyty mononukleární MeSH
- lidé MeSH
- přeprogramování buněk MeSH
- zárodečné listy MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.
Citace poskytuje Crossref.org
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