Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
34088007
DOI
10.1016/j.scr.2021.102378
PII: S1873-5061(21)00224-5
Knihovny.cz E-resources
- MeSH
- Alzheimer Disease * MeSH
- Cell Differentiation MeSH
- Induced Pluripotent Stem Cells * MeSH
- Leukocytes, Mononuclear MeSH
- Humans MeSH
- Cellular Reprogramming MeSH
- Germ Layers MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.
References provided by Crossref.org
In vitro human cell culture models in a bench-to-bedside approach to epilepsy
