-
Je něco špatně v tomto záznamu ?
Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein
F. Bianchini, V. Crivelli, ME. Abernathy, C. Guerra, M. Palus, J. Muri, H. Marcotte, A. Piralla, M. Pedotti, R. De Gasparo, L. Simonelli, M. Matkovic, C. Toscano, M. Biggiogero, V. Calvaruso, P. Svoboda, T. Cervantes Rincón, T. Fava, L....
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, N.I.H., Extramural
Grantová podpora
196866
Swiss National Science Foundation - Switzerland
P01 AI138938
NIAID NIH HHS - United States
U01 AI151698
NIAID NIH HHS - United States
U19 AI111825
NIAID NIH HHS - United States
- MeSH
- COVID-19 * MeSH
- epitopy MeSH
- glykoprotein S, koronavirus MeSH
- lidé MeSH
- myši MeSH
- neutralizační testy MeSH
- neutralizující protilátky * MeSH
- protilátky virové MeSH
- SARS-CoV-2 MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
Emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants diminishes the efficacy of vaccines and antiviral monoclonal antibodies. Continued development of immunotherapies and vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions of the virus spike glycoprotein that are both functionally relevant and averse to change, we identified human neutralizing antibodies to highly conserved viral epitopes. Antibody fp.006 binds the fusion peptide and cross-reacts against coronaviruses of the four genera, including the nine human coronaviruses, through recognition of a conserved motif that includes the S2' site of proteolytic cleavage. Antibody hr2.016 targets the stem helix and neutralizes SARS-CoV-2 variants. Antibody sd1.040 binds to subdomain 1, synergizes with antibody rbd.042 for neutralization, and, similar to fp.006 and hr2.016, protects mice expressing human angiotensin-converting enzyme 2 against infection when present as a bispecific antibody. Thus, coldspot-guided antibody discovery reveals donor-derived neutralizing antibodies that are cross-reactive with Orthocoronavirinae, including SARS-CoV-2 variants.
Chan Zuckerberg Biohub San Francisco CA USA
Clinical Research Unit Clinica Luganese Moncucco Lugano Switzerland
Department of Biology Stanford University Stanford CA USA
Department of Biosciences and Nutrition Karolinska Institutet Huddinge Sweden
Department of Chemistry and Biochemistry Mendel University in Brno Brno Czech Republic
Department of Clinical Surgical Diagnostic and Pediatric Sciences University of Pavia Pavia Italy
Department of Experimental Biology Faculty of Science Masaryk University Brno Czech Republic
European Commission Joint Research Centre Ispra Italy
Faculty of Science University of South Bohemia Ceske Budejovice Czech Republic
Institute for Research in Biomedicine Università della Svizzera italiana Bellinzona Switzerland
Internal Medicine and Infectious Diseases Clinica Luganese Moncucco Lugano Switzerland
Microbiology and Virology Department Fondazione IRCCS Policlinico San Matteo Pavia Italy
Sarafan ChEM H Macromolecular Structure Knowledge Center Stanford University Stanford CA USA
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc23003784
- 003
- CZ-PrNML
- 005
- 20230425140901.0
- 007
- ta
- 008
- 230418s2023 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1126/sciimmunol.ade0958 $2 doi
- 035 __
- $a (PubMed)36701425
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Bianchini, Filippo $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000207467629
- 245 10
- $a Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein / $c F. Bianchini, V. Crivelli, ME. Abernathy, C. Guerra, M. Palus, J. Muri, H. Marcotte, A. Piralla, M. Pedotti, R. De Gasparo, L. Simonelli, M. Matkovic, C. Toscano, M. Biggiogero, V. Calvaruso, P. Svoboda, T. Cervantes Rincón, T. Fava, L. Podešvová, AA. Shanbhag, A. Celoria, J. Sgrignani, M. Stefanik, V. Hönig, V. Pranclova, T. Michalcikova, J. Prochazka, G. Guerrini, D. Mehn, A. Ciabattini, H. Abolhassani, D. Jarrossay, M. Uguccioni, D. Medaglini, Q. Pan-Hammarström, L. Calzolai, D. Fernandez, F. Baldanti, A. Franzetti-Pellanda, C. Garzoni, R. Sedlacek, D. Ruzek, L. Varani, A. Cavalli, CO. Barnes, DF. Robbiani
- 520 9_
- $a Emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants diminishes the efficacy of vaccines and antiviral monoclonal antibodies. Continued development of immunotherapies and vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions of the virus spike glycoprotein that are both functionally relevant and averse to change, we identified human neutralizing antibodies to highly conserved viral epitopes. Antibody fp.006 binds the fusion peptide and cross-reacts against coronaviruses of the four genera, including the nine human coronaviruses, through recognition of a conserved motif that includes the S2' site of proteolytic cleavage. Antibody hr2.016 targets the stem helix and neutralizes SARS-CoV-2 variants. Antibody sd1.040 binds to subdomain 1, synergizes with antibody rbd.042 for neutralization, and, similar to fp.006 and hr2.016, protects mice expressing human angiotensin-converting enzyme 2 against infection when present as a bispecific antibody. Thus, coldspot-guided antibody discovery reveals donor-derived neutralizing antibodies that are cross-reactive with Orthocoronavirinae, including SARS-CoV-2 variants.
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a myši $7 D051379
- 650 12
- $a neutralizující protilátky $7 D057134
- 650 _2
- $a SARS-CoV-2 $7 D000086402
- 650 _2
- $a epitopy $7 D000939
- 650 _2
- $a glykoprotein S, koronavirus $7 D064370
- 650 _2
- $a protilátky virové $7 D000914
- 650 _2
- $a neutralizační testy $7 D009500
- 650 12
- $a COVID-19 $7 D000086382
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 655 _2
- $a Research Support, N.I.H., Extramural $7 D052061
- 700 1_
- $a Crivelli, Virginia $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000324947242
- 700 1_
- $a Abernathy, Morgan E $u Department of Biology, Stanford University, Stanford, CA, USA
- 700 1_
- $a Guerra, Concetta $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000155976885
- 700 1_
- $a Palus, Martin $u Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic $u Veterinary Research Institute, Brno, Czech Republic $1 https://orcid.org/0000000242797092
- 700 1_
- $a Muri, Jonathan $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland
- 700 1_
- $a Marcotte, Harold $u Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden $1 https://orcid.org/000000027168349X
- 700 1_
- $a Piralla, Antonio $u Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy $1 https://orcid.org/0000000260622579
- 700 1_
- $a Pedotti, Mattia $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000313709505
- 700 1_
- $a De Gasparo, Raoul $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000279348290
- 700 1_
- $a Simonelli, Luca $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland
- 700 1_
- $a Matkovic, Milos $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000248721996
- 700 1_
- $a Toscano, Chiara $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/000000020309946X
- 700 1_
- $a Biggiogero, Maira $u Clinical Research Unit, Clinica Luganese Moncucco, Lugano, Switzerland
- 700 1_
- $a Calvaruso, Veronica $u Clinical Research Unit, Clinica Luganese Moncucco, Lugano, Switzerland
- 700 1_
- $a Svoboda, Pavel $u Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic $u Veterinary Research Institute, Brno, Czech Republic $u Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic $u Department of Pharmacology and Pharmacy, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic $1 https://orcid.org/0000000221803174
- 700 1_
- $a Cervantes Rincón, Tomás $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000263556781
- 700 1_
- $a Fava, Tommaso $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000327675641
- 700 1_
- $a Podešvová, Lucie $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000310542252
- 700 1_
- $a Shanbhag, Akanksha A $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000216762478
- 700 1_
- $a Celoria, Andrea $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000261078296
- 700 1_
- $a Sgrignani, Jacopo $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland
- 700 1_
- $a Stefanik, Michal $u Veterinary Research Institute, Brno, Czech Republic $u Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic $1 https://orcid.org/0000000343907780
- 700 1_
- $a Hönig, Vaclav $u Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic $u Veterinary Research Institute, Brno, Czech Republic $1 https://orcid.org/0000000304694604 $7 jcu2012681186
- 700 1_
- $a Pranclova, Veronika $u Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic $u Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic $1 https://orcid.org/0000000316601372
- 700 1_
- $a Michalcikova, Tereza $u Czech Centre of Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vestec, Czech Republic $1 https://orcid.org/0000000201722254
- 700 1_
- $a Prochazka, Jan $u Czech Centre of Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vestec, Czech Republic
- 700 1_
- $a Guerrini, Giuditta $u European Commission, Joint Research Centre (JRC), Ispra Italy $1 https://orcid.org/0000000214460556
- 700 1_
- $a Mehn, Dora $u European Commission, Joint Research Centre (JRC), Ispra Italy $1 https://orcid.org/0000000334822996
- 700 1_
- $a Ciabattini, Annalisa $u Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, Siena, Italy $1 https://orcid.org/0000000245857783
- 700 1_
- $a Abolhassani, Hassan $u Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden $u Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran $1 https://orcid.org/0000000248380407
- 700 1_
- $a Jarrossay, David $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000209246395
- 700 1_
- $a Uguccioni, Mariagrazia $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000295707011
- 700 1_
- $a Medaglini, Donata $u Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, Siena, Italy
- 700 1_
- $a Pan-Hammarström, Qiang $u Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden $1 https://orcid.org/0000000319908804
- 700 1_
- $a Calzolai, Luigi $u European Commission, Joint Research Centre (JRC), Ispra Italy $1 https://orcid.org/0000000284747974
- 700 1_
- $a Fernandez, Daniel $u Sarafan ChEM-H Macromolecular Structure Knowledge Center, Stanford University, Stanford, CA, USA $1 https://orcid.org/000000026221152X
- 700 1_
- $a Baldanti, Fausto $u Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy $u Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy $1 https://orcid.org/0000000233588969
- 700 1_
- $a Franzetti-Pellanda, Alessandra $u Clinical Research Unit, Clinica Luganese Moncucco, Lugano, Switzerland $1 https://orcid.org/0000000207692500
- 700 1_
- $a Garzoni, Christian $u Internal Medicine and Infectious Diseases, Clinica Luganese Moncucco, Lugano, Switzerland $1 https://orcid.org/0000000208322376
- 700 1_
- $a Sedlacek, Radislav $u Czech Centre of Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vestec, Czech Republic $1 https://orcid.org/000000023352392X $7 xx0140532
- 700 1_
- $a Ruzek, Daniel $u Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic $u Veterinary Research Institute, Brno, Czech Republic $u Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic $1 https://orcid.org/0000000346552380 $7 stk2008441707
- 700 1_
- $a Varani, Luca $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000209630987
- 700 1_
- $a Cavalli, Andrea $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $u Swiss Institute of Bioinformatics, Lausanne, Switzerland $1 https://orcid.org/0000000340634502
- 700 1_
- $a Barnes, Christopher O $u Department of Biology, Stanford University, Stanford, CA, USA $u Chan Zuckerberg Biohub, San Francisco, CA, USA $1 https://orcid.org/0000000327545951
- 700 1_
- $a Robbiani, Davide F $u Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland $1 https://orcid.org/0000000173793484
- 773 0_
- $w MED00205752 $t Science immunology $x 2470-9468 $g Roč. 8, č. 81 (2023), s. eade0958
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/36701425 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20230418 $b ABA008
- 991 __
- $a 20230425140858 $b ABA008
- 999 __
- $a ok $b bmc $g 1924451 $s 1189993
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2023 $b 8 $c 81 $d eade0958 $e 20230310 $i 2470-9468 $m Science immunology $n Sci Immunol $x MED00205752
- GRA __
- $a 196866 $p Swiss National Science Foundation $2 Switzerland
- GRA __
- $a P01 AI138938 $p NIAID NIH HHS $2 United States
- GRA __
- $a U01 AI151698 $p NIAID NIH HHS $2 United States
- GRA __
- $a U19 AI111825 $p NIAID NIH HHS $2 United States
- LZP __
- $a Pubmed-20230418
