• Something wrong with this record ?

Cyanobacterial Harmful Bloom Lipopolysaccharides Induce Pro-Inflammatory Effects in Immune and Intestinal Epithelial Cells In Vitro

V. Skočková, O. Vašíček, E. Sychrová, I. Sovadinová, P. Babica, L. Šindlerová

. 2023 ; 15 (3) : . [pub] 20230221

Language English Country Switzerland

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc23004128

Freshwater cyanobacterial harmful blooms (CyanoHABs) produce a variety of toxic and bioactive compounds including lipopolysaccharides (LPSs). The gastrointestinal tract can be exposed to them via contaminated water even during recreational activities. However, there is no evidence of an effect of CyanoHAB LPSs on intestinal cells. We isolated LPSs of four CyanoHABs dominated by different cyanobacterial species and LPSs of four laboratory cultures representing the respective dominant cyanobacterial genera. Two intestinal and one macrophage cell lines were used to detect in vitro pro-inflammatory activity of the LPS. All LPSs isolated from CyanoHABs and laboratory cultures induced cytokines production in at least one in vitro model, except for LPSs from the Microcystis PCC7806 culture. LPSs isolated from cyanobacteria showed unique migration patterns in SDS-PAGE that were qualitatively distinct from those of endotoxins from Gram-negative bacteria. There was no clear relationship between the biological activity of the LPS and the share of genomic DNA of Gram-negative bacteria in the respective biomass. Thus, the total share of Gram-negative bacteria, or the presence of Escherichia coli-like LPSs, did not explain the observed pro-inflammatory activities. The pro-inflammatory properties of environmental mixtures of LPSs from CyanoHABs indicate their human health hazards, and further attention should be given to their assessment and monitoring.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23004128
003      
CZ-PrNML
005      
20230425141118.0
007      
ta
008      
230418s2023 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/toxins15030169 $2 doi
035    __
$a (PubMed)36977060
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Skočková, Veronika $u Department of Biophysics of Immune System, Institute of Biophysics of the Czech Academy of Sciences, 61200 Brno, Czech Republic $u Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic $1 https://orcid.org/0000000164299863
245    10
$a Cyanobacterial Harmful Bloom Lipopolysaccharides Induce Pro-Inflammatory Effects in Immune and Intestinal Epithelial Cells In Vitro / $c V. Skočková, O. Vašíček, E. Sychrová, I. Sovadinová, P. Babica, L. Šindlerová
520    9_
$a Freshwater cyanobacterial harmful blooms (CyanoHABs) produce a variety of toxic and bioactive compounds including lipopolysaccharides (LPSs). The gastrointestinal tract can be exposed to them via contaminated water even during recreational activities. However, there is no evidence of an effect of CyanoHAB LPSs on intestinal cells. We isolated LPSs of four CyanoHABs dominated by different cyanobacterial species and LPSs of four laboratory cultures representing the respective dominant cyanobacterial genera. Two intestinal and one macrophage cell lines were used to detect in vitro pro-inflammatory activity of the LPS. All LPSs isolated from CyanoHABs and laboratory cultures induced cytokines production in at least one in vitro model, except for LPSs from the Microcystis PCC7806 culture. LPSs isolated from cyanobacteria showed unique migration patterns in SDS-PAGE that were qualitatively distinct from those of endotoxins from Gram-negative bacteria. There was no clear relationship between the biological activity of the LPS and the share of genomic DNA of Gram-negative bacteria in the respective biomass. Thus, the total share of Gram-negative bacteria, or the presence of Escherichia coli-like LPSs, did not explain the observed pro-inflammatory activities. The pro-inflammatory properties of environmental mixtures of LPSs from CyanoHABs indicate their human health hazards, and further attention should be given to their assessment and monitoring.
650    _2
$a lidé $7 D006801
650    _2
$a lipopolysacharidy $x farmakologie $7 D008070
650    12
$a sinice $x metabolismus $7 D000458
650    12
$a Microcystis $7 D046931
650    _2
$a endotoxiny $x metabolismus $7 D004731
650    _2
$a sladká voda $x mikrobiologie $7 D005618
650    _2
$a škodlivý vodní květ $7 D057097
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Vašíček, Ondřej $u Department of Biophysics of Immune System, Institute of Biophysics of the Czech Academy of Sciences, 61200 Brno, Czech Republic $1 https://orcid.org/0000000158920457
700    1_
$a Sychrová, Eliška $u RECETOX, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic $1 https://orcid.org/000000029741196X
700    1_
$a Sovadinová, Iva $u RECETOX, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic $1 https://orcid.org/000000030627243X $7 xx0140851
700    1_
$a Babica, Pavel $u RECETOX, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic $u Department of Experimental Phycology and Ecotoxicology, Institute of Botany, 60200 Brno, Czech Republic $1 https://orcid.org/0000000163993554 $7 xx0084399
700    1_
$a Šindlerová, Lenka $u Department of Biophysics of Immune System, Institute of Biophysics of the Czech Academy of Sciences, 61200 Brno, Czech Republic $1 https://orcid.org/0000000188356703 $7 xx0121174
773    0_
$w MED00177194 $t Toxins $x 2072-6651 $g Roč. 15, č. 3 (2023)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/36977060 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230418 $b ABA008
991    __
$a 20230425141115 $b ABA008
999    __
$a ok $b bmc $g 1924660 $s 1190337
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 15 $c 3 $e 20230221 $i 2072-6651 $m Toxins $n Toxins $x MED00177194
LZP    __
$a Pubmed-20230418

Find record

Citation metrics

Archiving options