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Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene

B. Filipić, M. Malešević, Z. Vasiljević, K. Novović, M. Kojić, B. Jovčić

. 2023 ; 68 (3) : 431-440. [pub] 20221226

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc23008933

Grantová podpora
451-03-68/2022-14/200161 Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
451-03-68/2022-14/200042 Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
451-03-68/2022-14/200178 Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja

Trimethoprim-sulfamethoxazole (SXT) is the preferable treatment option of the infections caused by Achromobacter spp. Our study aimed to analyze the SXT resistance of 98 Achromobacter spp. isolates from pediatric patients, among which 33 isolates were SXT-resistant. The presence of intI1 was screened by PCR and genome sequence analyses. The intI1 gene was detected in 10 of SXT-resistant isolates that had shorter intI1 PCR fragments named intI1S. Structural changes in intI1S were confirmed by genome sequencing and analyses which revealed 86 amino acids deletion in IntI1S protein compared to canonical IntI1 protein. All IntI1S isolates were of non-CF origin. Pan-genome analysis of intI1S bearing A. xylosoxidans isolates comprised 9052 genes, with the core genome consisting of 5455 protein-coding genes. Results in this study indicate that IntI1S isolates were derived from clinical settings and that cystic fibrosis (CF) patients were potential reservoirs for healthcare-associated infections that occurred in non-CF patients.

Citace poskytuje Crossref.org

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