Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Amesia hispanica sp. nov., Producer of the Antifungal Class of Antibiotics Dactylfungins

E. Charria-Girón, AM. Stchigel, A. Čmoková, M. Kolařík, F. Surup, Y. Marin-Felix

. 2023 ; 9 (4) : . [pub] 20230412

Status not-indexed Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc23010002

Grant support
HZI POF IV Cooperativity and Creativity Project Call Helmholtz Centre for Infection Research
Project-ID 490821847 Deutsche Forschungsgemeinschaft
MSCA-RISE grant no. 101008129, Acronym: MYCOBIOMICS European Union's H2020 Research and Innovation Staff Exchange program
SEA-Europe Grant number JFS20ST-127, Acronym: Antiviralfun Southeast Asia - Europe Joint Funding Scheme

During a study of the diversity of soilborne fungi from Spain, a strain belonging to the family Chaetomiaceae (Sordariales) was isolated. The multigene phylogenetic inference using five DNA loci showed that this strain represents an undescribed species of the genus Amesia, herein introduced as A. hispanica sp. nov. Investigation of its secondary metabolome led to the isolation of two new derivatives (2 and 3) of the known antifungal antibiotic dactylfungin A (1), together with the known compound cochliodinol (4). The planar structures of 1-4 were determined by ultrahigh performance liquid chromatography coupled with diode array detection and ion mobility tandem mass spectrometry (UHPLC-DAD-IM-MS/MS) and extensive 1D and 2D nuclear magnetic resonance (NMR) spectroscopy after isolation by HPLC. All isolated secondary metabolites were tested for their antimicrobial and cytotoxic activities. Dactylfungin A (1) showed selective and strong antifungal activity against some of the tested human pathogens (Aspergillus fumigatus and Cryptococcus neoformans). The additional hydroxyl group in 2 resulted in the loss of activity against C. neoformans but still retained the inhibition of As. fumigatus in a lower concentration than that of the respective control, without showing any cytotoxic effects. In contrast, 25′′-dehydroxy-dactylfungin A (3) exhibited improved activity against yeasts (Schizosaccharomyces pombe and Rhodotorula glutinis) than 1 and 2, but resulted in the appearance of slight cytotoxicity. The present study exemplifies how even in a well-studied taxonomic group such as the Chaetomiaceae, the investigation of novel taxa still brings chemistry novelty, as demonstrated in this first report of this antibiotic class for chaetomiaceous and sordarialean taxa.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23010002
003      
CZ-PrNML
005      
20230721095349.0
007      
ta
008      
230707s2023 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/jof9040463 $2 doi
035    __
$a (PubMed)37108917
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Charria-Girón, Esteban $u Department Microbial Drugs, Helmholtz Centre for Infection Research (HZI), German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany $u Institute of Microbiology, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany $1 https://orcid.org/0000000238231416
245    10
$a Amesia hispanica sp. nov., Producer of the Antifungal Class of Antibiotics Dactylfungins / $c E. Charria-Girón, AM. Stchigel, A. Čmoková, M. Kolařík, F. Surup, Y. Marin-Felix
520    9_
$a During a study of the diversity of soilborne fungi from Spain, a strain belonging to the family Chaetomiaceae (Sordariales) was isolated. The multigene phylogenetic inference using five DNA loci showed that this strain represents an undescribed species of the genus Amesia, herein introduced as A. hispanica sp. nov. Investigation of its secondary metabolome led to the isolation of two new derivatives (2 and 3) of the known antifungal antibiotic dactylfungin A (1), together with the known compound cochliodinol (4). The planar structures of 1-4 were determined by ultrahigh performance liquid chromatography coupled with diode array detection and ion mobility tandem mass spectrometry (UHPLC-DAD-IM-MS/MS) and extensive 1D and 2D nuclear magnetic resonance (NMR) spectroscopy after isolation by HPLC. All isolated secondary metabolites were tested for their antimicrobial and cytotoxic activities. Dactylfungin A (1) showed selective and strong antifungal activity against some of the tested human pathogens (Aspergillus fumigatus and Cryptococcus neoformans). The additional hydroxyl group in 2 resulted in the loss of activity against C. neoformans but still retained the inhibition of As. fumigatus in a lower concentration than that of the respective control, without showing any cytotoxic effects. In contrast, 25′′-dehydroxy-dactylfungin A (3) exhibited improved activity against yeasts (Schizosaccharomyces pombe and Rhodotorula glutinis) than 1 and 2, but resulted in the appearance of slight cytotoxicity. The present study exemplifies how even in a well-studied taxonomic group such as the Chaetomiaceae, the investigation of novel taxa still brings chemistry novelty, as demonstrated in this first report of this antibiotic class for chaetomiaceous and sordarialean taxa.
590    __
$a NEINDEXOVÁNO
655    _2
$a časopisecké články $7 D016428
700    1_
$a Stchigel, Alberto Miguel $u Mycology Unit, Medical School, Universitat Rovira i Virgili, C/Sant Llorenç 21, 43201 Tarragona, Spain $1 https://orcid.org/0000000339877996
700    1_
$a Čmoková, Adéla $u Institute of Microbiology, Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic
700    1_
$a Kolařík, Miroslav $u Institute of Microbiology, Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic $1 https://orcid.org/0000000340160335 $7 xx0234047
700    1_
$a Surup, Frank $u Department Microbial Drugs, Helmholtz Centre for Infection Research (HZI), German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany $u Institute of Microbiology, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany $1 https://orcid.org/0000000152348525
700    1_
$a Marin-Felix, Yasmina $u Department Microbial Drugs, Helmholtz Centre for Infection Research (HZI), German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany $u Institute of Microbiology, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany
773    0_
$w MED00203318 $t Journal of fungi (Basel, Switzerland) $x 2309-608X $g Roč. 9, č. 4 (2023)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37108917 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230707 $b ABA008
991    __
$a 20230721095342 $b ABA008
999    __
$a ok $b bmc $g 1958599 $s 1196266
BAS    __
$a 3
BAS    __
$a PreBMC-PubMed-not-MEDLINE
BMC    __
$a 2023 $b 9 $c 4 $e 20230412 $i 2309-608X $m Journal of fungi $n J Fungi (Basel) $x MED00203318
GRA    __
$a HZI POF IV Cooperativity and Creativity Project Call $p Helmholtz Centre for Infection Research
GRA    __
$a Project-ID 490821847 $p Deutsche Forschungsgemeinschaft
GRA    __
$a MSCA-RISE grant no. 101008129, Acronym: MYCOBIOMICS $p European Union's H2020 Research and Innovation Staff Exchange program
GRA    __
$a SEA-Europe Grant number JFS20ST-127, Acronym: Antiviralfun $p Southeast Asia - Europe Joint Funding Scheme
LZP    __
$a Pubmed-20230707

Find record

Citation metrics

Archiving options