-
Je něco špatně v tomto záznamu ?
Dynamics of extracellular vesicle-coupled microRNAs in equine follicular fluid associated with follicle selection and ovulation
S. Gebremedhn, A. Gad, GM. Ishak, NG. Menjivar, MO. Gastal, JM. Feugang, R. Prochazka, D. Tesfaye, EL. Gastal
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, N.I.H., Extramural
Grantová podpora
Ministry of Higher Education & Scientific Research, Baghdad
Open Access Digital Library od 1996-01-01
Medline Complete (EBSCOhost) od 1995-01-01 do Před 1 rokem
Odkazy
PubMed
36852862
DOI
10.1093/molehr/gaad009
Knihovny.cz E-zdroje
- MeSH
- extracelulární vezikuly * genetika metabolismus MeSH
- folikulární tekutina metabolismus MeSH
- koně MeSH
- lidé MeSH
- mikro RNA * metabolismus MeSH
- ovariální folikul metabolismus MeSH
- ovulace genetika MeSH
- savci MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
Innumerable similarities in reproductive cyclicity and hormonal alterations highlight the considerable utility of the mare to study aspects of follicular dynamics and reproductive function in view of the largely constricted, human research subjects. The bi-directional communication between the growing oocyte and the surrounding somatic cells embodies the hallmark of mammalian follicular development, partially mediated by extracellular vesicles (EVs) encapsulated with microRNAs (miRNAs) and present in the follicular fluid (FF). Here, we aimed to decipher the dynamics of the miRNAs in EVs from equine FF aspirated in vivo during different stages of follicular development, namely, predeviation (PreDev; 18-20 mm), deviation (Dev; 22-25 mm), postdeviation (PostDev; 26-29 mm), preovulatory (PreOV; 30-35 mm), and impending ovulation (IMP; ∼40 mm). Approximately 176 known miRNAs were found in all groups with 144 mutually detected among all groups. Cluster analysis exhibited 15 different expression patterns during follicular development. Among these patterns, a group of 22 miRNAs (including miR-146b-5p, miR-140, and miR-143) exhibited a sharp reduction in expression from the PreDev until the PreOV stage. Another cluster of 23 miRNAs (including miR-106b, miR-199a-5p, and miR-125a-5p) exhibited a stable expression pattern at the PreDev stage until the PostDev stage, with a significant increase at the PreOV stage followed by a significant decrease at the IMP stage. In conclusion, this study provides greater insights into the stage-specific expression dynamics of FF EV-miRNAs during equine follicular development, which may propose novel approaches to improve ART and provide new biomarkers to facilitate the assessment of ovarian pathophysiological conditions.
Department of Animal and Dairy Sciences Mississippi State University Mississippi State MS USA
Department of Animal Production Faculty of Agriculture Cairo University Giza Egypt
- 000
- 00000naa a2200000 a 4500
- 001
- bmc23010505
- 003
- CZ-PrNML
- 005
- 20230801132452.0
- 007
- ta
- 008
- 230718s2023 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1093/molehr/gaad009 $2 doi
- 035 __
- $a (PubMed)36852862
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Gebremedhn, Samuel $u Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA $u Division of IVF Research, Genus Plc, DeForest, WI, USA
- 245 10
- $a Dynamics of extracellular vesicle-coupled microRNAs in equine follicular fluid associated with follicle selection and ovulation / $c S. Gebremedhn, A. Gad, GM. Ishak, NG. Menjivar, MO. Gastal, JM. Feugang, R. Prochazka, D. Tesfaye, EL. Gastal
- 520 9_
- $a Innumerable similarities in reproductive cyclicity and hormonal alterations highlight the considerable utility of the mare to study aspects of follicular dynamics and reproductive function in view of the largely constricted, human research subjects. The bi-directional communication between the growing oocyte and the surrounding somatic cells embodies the hallmark of mammalian follicular development, partially mediated by extracellular vesicles (EVs) encapsulated with microRNAs (miRNAs) and present in the follicular fluid (FF). Here, we aimed to decipher the dynamics of the miRNAs in EVs from equine FF aspirated in vivo during different stages of follicular development, namely, predeviation (PreDev; 18-20 mm), deviation (Dev; 22-25 mm), postdeviation (PostDev; 26-29 mm), preovulatory (PreOV; 30-35 mm), and impending ovulation (IMP; ∼40 mm). Approximately 176 known miRNAs were found in all groups with 144 mutually detected among all groups. Cluster analysis exhibited 15 different expression patterns during follicular development. Among these patterns, a group of 22 miRNAs (including miR-146b-5p, miR-140, and miR-143) exhibited a sharp reduction in expression from the PreDev until the PreOV stage. Another cluster of 23 miRNAs (including miR-106b, miR-199a-5p, and miR-125a-5p) exhibited a stable expression pattern at the PreDev stage until the PostDev stage, with a significant increase at the PreOV stage followed by a significant decrease at the IMP stage. In conclusion, this study provides greater insights into the stage-specific expression dynamics of FF EV-miRNAs during equine follicular development, which may propose novel approaches to improve ART and provide new biomarkers to facilitate the assessment of ovarian pathophysiological conditions.
- 650 _2
- $a koně $7 D006736
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a folikulární tekutina $x metabolismus $7 D015571
- 650 12
- $a mikro RNA $x metabolismus $7 D035683
- 650 _2
- $a ovariální folikul $x metabolismus $7 D006080
- 650 _2
- $a ovulace $x genetika $7 D010060
- 650 12
- $a extracelulární vezikuly $x genetika $x metabolismus $7 D000067128
- 650 _2
- $a savci $7 D008322
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 655 _2
- $a Research Support, N.I.H., Extramural $7 D052061
- 700 1_
- $a Gad, Ahmed $u Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA $u Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt
- 700 1_
- $a Ishak, Ghassan M $u Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq $u Department of Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, USA
- 700 1_
- $a Menjivar, Nico G $u Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA
- 700 1_
- $a Gastal, Melba O $u Department of Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, USA
- 700 1_
- $a Feugang, Jean M $u Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, USA
- 700 1_
- $a Prochazka, Radek $u Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic
- 700 1_
- $a Tesfaye, Dawit $u Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA $1 https://orcid.org/0000000181660606
- 700 1_
- $a Gastal, Eduardo L $u Department of Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, USA $1 https://orcid.org/0000000288556980
- 773 0_
- $w MED00005120 $t Molecular human reproduction $x 1460-2407 $g Roč. 29, č. 4 (2023)
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/36852862 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20230718 $b ABA008
- 991 __
- $a 20230801132449 $b ABA008
- 999 __
- $a ok $b bmc $g 1963120 $s 1196770
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2023 $b 29 $c 4 $e 2023Apr03 $i 1460-2407 $m Molecular human reproduction $n Mol Hum Reprod $x MED00005120
- GRA __
- $p Ministry of Higher Education & Scientific Research, Baghdad
- LZP __
- $a Pubmed-20230718