• Je něco špatně v tomto záznamu ?

Evolutionary analysis of cellular reduction and anaerobicity in the hyper-prevalent gut microbe Blastocystis

K. Záhonová, RS. Low, CJ. Warren, D. Cantoni, EK. Herman, L. Yiangou, CA. Ribeiro, Y. Phanprasert, IR. Brown, S. Rueckert, NL. Baker, J. Tachezy, EL. Betts, E. Gentekaki, M. van der Giezen, CG. Clark, AP. Jackson, JB. Dacks, AD. Tsaousis

. 2023 ; 33 (12) : 2449-2464.e8. [pub] 20230601

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc23011026
E-zdroje Online Plný text

NLK Cell Press Free Archives od 1995-01-01 do Před 1 rokem
Free Medical Journals od 1995 do Před 1 rokem
Elsevier Open Access Journals od 1995-01-01 do 2023-06-19
Elsevier Open Archive Journals od 1995-01-01 do Před 1 rokem

Blastocystis is the most prevalent microbial eukaryote in the human and animal gut, yet its role as commensal or parasite is still under debate. Blastocystis has clearly undergone evolutionary adaptation to the gut environment and possesses minimal cellular compartmentalization, reduced anaerobic mitochondria, no flagella, and no reported peroxisomes. To address this poorly understood evolutionary transition, we have taken a multi-disciplinary approach to characterize Proteromonas lacertae, the closest canonical stramenopile relative of Blastocystis. Genomic data reveal an abundance of unique genes in P. lacertae but also reductive evolution of the genomic complement in Blastocystis. Comparative genomic analysis sheds light on flagellar evolution, including 37 new candidate components implicated with mastigonemes, the stramenopile morphological hallmark. The P. lacertae membrane-trafficking system (MTS) complement is only slightly more canonical than that of Blastocystis, but notably, we identified that both organisms encode the complete enigmatic endocytic TSET complex, a first for the entire stramenopile lineage. Investigation also details the modulation of mitochondrial composition and metabolism in both P. lacertae and Blastocystis. Unexpectedly, we identify in P. lacertae the most reduced peroxisome-derived organelle reported to date, which leads us to speculate on a mechanism of constraint guiding the dynamics of peroxisome-mitochondrion reductive evolution on the path to anaerobiosis. Overall, these analyses provide a launching point to investigate organellar evolution and reveal in detail the evolutionary path that Blastocystis has taken from a canonical flagellated protist to the hyper-divergent and hyper-prevalent animal and human gut microbe.

Biosciences University of Exeter Stocker Road Exeter EX4 4QD UK

Centre for Life's Origin and Evolution Division of Biosciences University College London Darwin Building Gower Street London WC1E 6BT UK

Department of Agricultural Food and Nutritional Science Faculty of Agricultural Life and Environmental Sciences University of Alberta 2 31 General Services Building Edmonton AB T6G 2H1 Canada

Department of Chemistry Bioscience and Environmental Engineering University of Stavanger Richard Johnsens Gate 4 4021 Stavanger Norway

Department of Infection Biology Faculty of Infectious and Tropical Diseases London School of Hygiene and Tropical Medicine Keppel Street London WC1E 7HT UK

Department of Parasitology Faculty of Science Charles University BIOCEV Průmyslová 595 Vestec 252 50 Czech Republic

Division of Infectious Diseases Department of Medicine University of Alberta 1 124 Clinical Sciences Building 11350 83 Avenue Edmonton T6G 2G3 Canada

Faculty of Biology AG Eukaryotische Mikrobiologie Universitätsstrasse 5 S05 R04 H83 Essen 45141 Germany

Gut Microbiome Research Group Mae Fah Luang University 333 Moo 1 T Tasud Muang District Chiang Rai 57100 Thailand

Institute of Infection Veterinary and Ecological Sciences University of Liverpool Liverpool UK

Institute of Parasitology Biology Centre Czech Academy of Sciences Branišovská 1160 31 České Budějovice 370 05 Czech Republic

Laboratory of Molecular and Evolutionary Parasitology RAPID Group School of Biosciences University of Kent Giles Lane Stacey Building Canterbury Kent CT2 7NJ UK

Life Science Research Centre Department of Biology and Ecology Faculty of Science University of Ostrava Chittussiho 10 Ostrava 710 00 Czech Republic

School of Applied Sciences Sighthill Campus Room 3 B 36 Edinburgh EH11 4BN Scotland

School of Science Mae Fah Luang Universit 333 Moo 1 T Tasud Muang District Chiang Rai 57100 Thailand

The Earlham Institute Norwich Research Park Norwich NR4 7UZ UK

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23011026
003      
CZ-PrNML
005      
20230801132750.0
007      
ta
008      
230718s2023 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.cub.2023.05.025 $2 doi
035    __
$a (PubMed)37267944
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Záhonová, Kristína $u Division of Infectious Diseases, Department of Medicine, University of Alberta, 1-124 Clinical Sciences Building, 11350-83 Avenue, Edmonton T6G 2G3, Canada; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branišovská 1160/31, České Budějovice (Budweis) 370 05, Czech Republic; Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Průmyslová 595, Vestec 252 50, Czech Republic; Life Science Research Centre, Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, Ostrava 710 00, Czech Republic
245    10
$a Evolutionary analysis of cellular reduction and anaerobicity in the hyper-prevalent gut microbe Blastocystis / $c K. Záhonová, RS. Low, CJ. Warren, D. Cantoni, EK. Herman, L. Yiangou, CA. Ribeiro, Y. Phanprasert, IR. Brown, S. Rueckert, NL. Baker, J. Tachezy, EL. Betts, E. Gentekaki, M. van der Giezen, CG. Clark, AP. Jackson, JB. Dacks, AD. Tsaousis
520    9_
$a Blastocystis is the most prevalent microbial eukaryote in the human and animal gut, yet its role as commensal or parasite is still under debate. Blastocystis has clearly undergone evolutionary adaptation to the gut environment and possesses minimal cellular compartmentalization, reduced anaerobic mitochondria, no flagella, and no reported peroxisomes. To address this poorly understood evolutionary transition, we have taken a multi-disciplinary approach to characterize Proteromonas lacertae, the closest canonical stramenopile relative of Blastocystis. Genomic data reveal an abundance of unique genes in P. lacertae but also reductive evolution of the genomic complement in Blastocystis. Comparative genomic analysis sheds light on flagellar evolution, including 37 new candidate components implicated with mastigonemes, the stramenopile morphological hallmark. The P. lacertae membrane-trafficking system (MTS) complement is only slightly more canonical than that of Blastocystis, but notably, we identified that both organisms encode the complete enigmatic endocytic TSET complex, a first for the entire stramenopile lineage. Investigation also details the modulation of mitochondrial composition and metabolism in both P. lacertae and Blastocystis. Unexpectedly, we identify in P. lacertae the most reduced peroxisome-derived organelle reported to date, which leads us to speculate on a mechanism of constraint guiding the dynamics of peroxisome-mitochondrion reductive evolution on the path to anaerobiosis. Overall, these analyses provide a launching point to investigate organellar evolution and reveal in detail the evolutionary path that Blastocystis has taken from a canonical flagellated protist to the hyper-divergent and hyper-prevalent animal and human gut microbe.
650    _2
$a zvířata $7 D000818
650    _2
$a lidé $7 D006801
650    12
$a Blastocystis $x genetika $7 D016844
650    12
$a střevní mikroflóra $x genetika $7 D000069196
650    _2
$a mitochondrie $x genetika $x metabolismus $7 D008928
650    _2
$a organely $x metabolismus $7 D015388
650    _2
$a Eukaryota $7 D056890
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Low, Ross S $u Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, UK; The Earlham Institute, Norwich Research Park, Norwich NR4 7UZ, UK
700    1_
$a Warren, Christopher J $u Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Giles Lane, Stacey Building, Canterbury, Kent CT2 7NJ, UK
700    1_
$a Cantoni, Diego $u Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Giles Lane, Stacey Building, Canterbury, Kent CT2 7NJ, UK
700    1_
$a Herman, Emily K $u Division of Infectious Diseases, Department of Medicine, University of Alberta, 1-124 Clinical Sciences Building, 11350-83 Avenue, Edmonton T6G 2G3, Canada; Department of Agricultural, Food, and Nutritional Science, Faculty of Agricultural, Life, and Environmental Sciences, University of Alberta, 2-31 General Services Building, Edmonton, AB T6G 2H1, Canada
700    1_
$a Yiangou, Lyto $u Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Giles Lane, Stacey Building, Canterbury, Kent CT2 7NJ, UK
700    1_
$a Ribeiro, Cláudia A $u Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Giles Lane, Stacey Building, Canterbury, Kent CT2 7NJ, UK
700    1_
$a Phanprasert, Yasinee $u Division of Infectious Diseases, Department of Medicine, University of Alberta, 1-124 Clinical Sciences Building, 11350-83 Avenue, Edmonton T6G 2G3, Canada; School of Science, Mae Fah Luang Universit, 333 Moo 1, T. Tasud, Muang District, Chiang Rai 57100, Thailand
700    1_
$a Brown, Ian R $u Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Giles Lane, Stacey Building, Canterbury, Kent CT2 7NJ, UK
700    1_
$a Rueckert, Sonja $u School of Applied Sciences, Sighthill Campus, Room 3.B.36, Edinburgh EH11 4BN, Scotland; Faculty of Biology, AG Eukaryotische Mikrobiologie, Universitätsstrasse 5, S05 R04 H83, Essen 45141, Germany
700    1_
$a Baker, Nicola L $u Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Giles Lane, Stacey Building, Canterbury, Kent CT2 7NJ, UK
700    1_
$a Tachezy, Jan $u Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Průmyslová 595, Vestec 252 50, Czech Republic
700    1_
$a Betts, Emma L $u Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Giles Lane, Stacey Building, Canterbury, Kent CT2 7NJ, UK; School of Applied Sciences, Sighthill Campus, Room 3.B.36, Edinburgh EH11 4BN, Scotland
700    1_
$a Gentekaki, Eleni $u School of Science, Mae Fah Luang Universit, 333 Moo 1, T. Tasud, Muang District, Chiang Rai 57100, Thailand; Gut Microbiome Research Group, Mae Fah Luang University, 333 Moo 1, T. Tasud, Muang District, Chiang Rai 57100, Thailand
700    1_
$a van der Giezen, Mark $u Department of Chemistry, Bioscience and Environmental Engineering, University of Stavanger Richard Johnsens Gate 4, 4021 Stavanger, Norway; Biosciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK
700    1_
$a Clark, C Graham $u Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, UK
700    1_
$a Jackson, Andrew P $u Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, UK
700    1_
$a Dacks, Joel B $u Division of Infectious Diseases, Department of Medicine, University of Alberta, 1-124 Clinical Sciences Building, 11350-83 Avenue, Edmonton T6G 2G3, Canada; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branišovská 1160/31, České Budějovice (Budweis) 370 05, Czech Republic; Centre for Life's Origin and Evolution, Division of Biosciences, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK. Electronic address: dacks@ualberta.ca
700    1_
$a Tsaousis, Anastasios D $u Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Giles Lane, Stacey Building, Canterbury, Kent CT2 7NJ, UK. Electronic address: a.tsaousis@kent.ac.uk
773    0_
$w MED00006482 $t Current biology : CB $x 1879-0445 $g Roč. 33, č. 12 (2023), s. 2449-2464.e8
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37267944 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230718 $b ABA008
991    __
$a 20230801132747 $b ABA008
999    __
$a ok $b bmc $g 1963437 $s 1197291
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 33 $c 12 $d 2449-2464.e8 $e 20230601 $i 1879-0445 $m Current biology $n Curr Biol $x MED00006482
LZP    __
$a Pubmed-20230718

Najít záznam

Citační ukazatele

Nahrávání dat ...

    Možnosti archivace