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Evolutionary analysis of cellular reduction and anaerobicity in the hyper-prevalent gut microbe Blastocystis
K. Záhonová, RS. Low, CJ. Warren, D. Cantoni, EK. Herman, L. Yiangou, CA. Ribeiro, Y. Phanprasert, IR. Brown, S. Rueckert, NL. Baker, J. Tachezy, EL. Betts, E. Gentekaki, M. van der Giezen, CG. Clark, AP. Jackson, JB. Dacks, AD. Tsaousis
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Cell Press Free Archives
od 1995-01-01 do Před 1 rokem
Free Medical Journals
od 1995 do Před 1 rokem
Elsevier Open Access Journals
od 1995-01-01 do 2023-06-19
Elsevier Open Archive Journals
od 1995-01-01 do Před 1 rokem
- MeSH
- Blastocystis * genetika MeSH
- Eukaryota MeSH
- lidé MeSH
- mitochondrie genetika metabolismus MeSH
- organely metabolismus MeSH
- střevní mikroflóra * genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Blastocystis is the most prevalent microbial eukaryote in the human and animal gut, yet its role as commensal or parasite is still under debate. Blastocystis has clearly undergone evolutionary adaptation to the gut environment and possesses minimal cellular compartmentalization, reduced anaerobic mitochondria, no flagella, and no reported peroxisomes. To address this poorly understood evolutionary transition, we have taken a multi-disciplinary approach to characterize Proteromonas lacertae, the closest canonical stramenopile relative of Blastocystis. Genomic data reveal an abundance of unique genes in P. lacertae but also reductive evolution of the genomic complement in Blastocystis. Comparative genomic analysis sheds light on flagellar evolution, including 37 new candidate components implicated with mastigonemes, the stramenopile morphological hallmark. The P. lacertae membrane-trafficking system (MTS) complement is only slightly more canonical than that of Blastocystis, but notably, we identified that both organisms encode the complete enigmatic endocytic TSET complex, a first for the entire stramenopile lineage. Investigation also details the modulation of mitochondrial composition and metabolism in both P. lacertae and Blastocystis. Unexpectedly, we identify in P. lacertae the most reduced peroxisome-derived organelle reported to date, which leads us to speculate on a mechanism of constraint guiding the dynamics of peroxisome-mitochondrion reductive evolution on the path to anaerobiosis. Overall, these analyses provide a launching point to investigate organellar evolution and reveal in detail the evolutionary path that Blastocystis has taken from a canonical flagellated protist to the hyper-divergent and hyper-prevalent animal and human gut microbe.
Biosciences University of Exeter Stocker Road Exeter EX4 4QD UK
Institute of Infection Veterinary and Ecological Sciences University of Liverpool Liverpool UK
School of Applied Sciences Sighthill Campus Room 3 B 36 Edinburgh EH11 4BN Scotland
School of Science Mae Fah Luang Universit 333 Moo 1 T Tasud Muang District Chiang Rai 57100 Thailand
The Earlham Institute Norwich Research Park Norwich NR4 7UZ UK
Citace poskytuje Crossref.org
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- $a Záhonová, Kristína $u Division of Infectious Diseases, Department of Medicine, University of Alberta, 1-124 Clinical Sciences Building, 11350-83 Avenue, Edmonton T6G 2G3, Canada; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branišovská 1160/31, České Budějovice (Budweis) 370 05, Czech Republic; Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Průmyslová 595, Vestec 252 50, Czech Republic; Life Science Research Centre, Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, Ostrava 710 00, Czech Republic
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