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Porcine oocytes matured in a chemically defined medium are transcriptionally active
M. Murin, L. Nemcova, A. Bartkova, A. Gad, A. Lucas-Hahn, F. Strejcek, R. Prochazka, J. Laurincik
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
- MeSH
- buněčné jádro metabolismus MeSH
- IVM techniky * veterinární metody MeSH
- messenger RNA genetika metabolismus MeSH
- oocyty * metabolismus MeSH
- prasata MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The statement that fully-grown porcine oocytes (oocytes from follicles with diameter from 3 to 6 mm) are transcriptionally quiescent is not as strongly supported as it was before. Currently, we know that there is a difference between the transcription profile of germinal vesicle (GV) and metaphase II (MII) oocytes. The goal of our study was to compare the transcription profile of GV, germinal vesicle breakdown (GVBD), metaphase I (MI), and MII oocytes matured in the chemically defined medium FLI. Oocytes were sequenced, and the results were subsequently validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR). We detected multiple differentially transcribed mRNAs, of which many were upregulated. Among them we found mRNAs necessary for protein production, mitochondrial functions and cytoplasmic maturation. Collectively, these data support the hypothesis that transcription activity in fully-grown porcine oocytes is necessary for key processes during their successful maturation in vitro in a chemically defined maturation medium.
Department of Animal Production Faculty of Agriculture Cairo University Giza 12613 Egypt
Friedrich Loeffler Institute Institute of Animal Genetics Holtystrasse 10 Neustadt D 31535 Germany
Citace poskytuje Crossref.org
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- $a Murin, Matej $u Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, 27721, Czech Republic. Electronic address: Murin@iapg.cas.cz
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- $a Porcine oocytes matured in a chemically defined medium are transcriptionally active / $c M. Murin, L. Nemcova, A. Bartkova, A. Gad, A. Lucas-Hahn, F. Strejcek, R. Prochazka, J. Laurincik
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- $a The statement that fully-grown porcine oocytes (oocytes from follicles with diameter from 3 to 6 mm) are transcriptionally quiescent is not as strongly supported as it was before. Currently, we know that there is a difference between the transcription profile of germinal vesicle (GV) and metaphase II (MII) oocytes. The goal of our study was to compare the transcription profile of GV, germinal vesicle breakdown (GVBD), metaphase I (MI), and MII oocytes matured in the chemically defined medium FLI. Oocytes were sequenced, and the results were subsequently validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR). We detected multiple differentially transcribed mRNAs, of which many were upregulated. Among them we found mRNAs necessary for protein production, mitochondrial functions and cytoplasmic maturation. Collectively, these data support the hypothesis that transcription activity in fully-grown porcine oocytes is necessary for key processes during their successful maturation in vitro in a chemically defined maturation medium.
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- $a Nemcova, Lucie $u Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, 27721, Czech Republic. Electronic address: Nemcova@iapg.cas.cz
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