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Stable endocytic structures navigate the complex pellicle of apicomplexan parasites

L. Koreny, BN. Mercado-Saavedra, CM. Klinger, K. Barylyuk, S. Butterworth, J. Hirst, Y. Rivera-Cuevas, NR. Zaccai, VJC. Holzer, A. Klingl, JB. Dacks, VB. Carruthers, MS. Robinson, S. Gras, RF. Waller

. 2023 ; 14 (1) : 2167. [pub] 20230415

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc23011768

Grantová podpora
214272 Wellcome Trust - United Kingdom
WT 207455/Z/17/Z Wellcome Trust - United Kingdom
T32 AI007528 NIAID NIH HHS - United States
100140 Wellcome Trust - United Kingdom
086598 Wellcome Trust - United Kingdom
R01 AI120607 NIAID NIH HHS - United States
F31 AI152297 NIAID NIH HHS - United States
214298/Z/18/Z Wellcome Trust - United Kingdom

Apicomplexan parasites have immense impacts on humanity, but their basic cellular processes are often poorly understood. Where endocytosis occurs in these cells, how conserved this process is with other eukaryotes, and what the functions of endocytosis are across this phylum are major unanswered questions. Using the apicomplexan model Toxoplasma, we identified the molecular composition and behavior of unusual, fixed endocytic structures. Here, stable complexes of endocytic proteins differ markedly from the dynamic assembly/disassembly of these machineries in other eukaryotes. We identify that these endocytic structures correspond to the 'micropore' that has been observed throughout the Apicomplexa. Moreover, conserved molecular adaptation of this structure is seen in apicomplexans including the kelch-domain protein K13 that is central to malarial drug-resistance. We determine that a dominant function of endocytosis in Toxoplasma is plasma membrane homeostasis, rather than parasite nutrition, and that these specialized endocytic structures originated early in infrakingdom Alveolata likely in response to the complex cell pellicle that defines this medically and ecologically important ancient eukaryotic lineage.

Citace poskytuje Crossref.org

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