• Je něco špatně v tomto záznamu ?

Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity

SM. Koch, C. Freidank-Pohl, O. Siontas, M. Cortesao, A. Mota, K. Runzheimer, S. Jung, K. Rebrosova, M. Siler, R. Moeller, V. Meyer

. 2023 ; 14 (-) : 1233740. [pub] 20230720

Status neindexováno Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc23015679

Melanins are complex pigments with various biological functions and potential applications in space exploration and biomedicine due to their radioprotective properties. Aspergillus niger, a fungus known for its high radiation resistance, is widely used in biotechnology and a candidate for melanin production. In this study, we investigated the production of fungal pyomelanin (PyoFun) in A. niger by inducing overproduction of the pigment using L-tyrosine in a recombinant ΔhmgA mutant strain (OS4.3). The PyoFun pigment was characterized using three spectroscopic methods, and its antioxidant properties were assessed using a DPPH-assay. Additionally, we evaluated the protective effect of PyoFun against non-ionizing radiation (monochromatic UV-C) and compared its efficacy to a synthetically produced control pyomelanin (PyoSyn). The results confirmed successful production of PyoFun in A. niger through inducible overproduction. Characterization using spectroscopic methods confirmed the presence of PyoFun, and the DPPH-assay demonstrated its strong antioxidant properties. Moreover, PyoFun exhibited a highly protective effect against radiation-induced stress, surpassing the protection provided by PyoSyn. The findings of this study suggest that PyoFun has significant potential as a biological shield against harmful radiation. Notably, PyoFun is synthesized extracellularly, differing it from other fungal melanins (such as L-DOPA- or DHN-melanin) that require cell lysis for pigment purification. This characteristic makes PyoFun a valuable resource for biotechnology, biomedicine, and the space industry. However, further research is needed to evaluate its protective effect in a dried form and against ionizing radiation.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23015679
003      
CZ-PrNML
005      
20231020093510.0
007      
ta
008      
231010s2023 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3389/fmicb.2023.1233740 $2 doi
035    __
$a (PubMed)37547691
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Koch, Stella Marie $u Radiation Biology Department, Aerospace Microbiology Research Group, German Aerospace Center, Institute of Aerospace Medicine, Cologne, Germany
245    10
$a Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity / $c SM. Koch, C. Freidank-Pohl, O. Siontas, M. Cortesao, A. Mota, K. Runzheimer, S. Jung, K. Rebrosova, M. Siler, R. Moeller, V. Meyer
520    9_
$a Melanins are complex pigments with various biological functions and potential applications in space exploration and biomedicine due to their radioprotective properties. Aspergillus niger, a fungus known for its high radiation resistance, is widely used in biotechnology and a candidate for melanin production. In this study, we investigated the production of fungal pyomelanin (PyoFun) in A. niger by inducing overproduction of the pigment using L-tyrosine in a recombinant ΔhmgA mutant strain (OS4.3). The PyoFun pigment was characterized using three spectroscopic methods, and its antioxidant properties were assessed using a DPPH-assay. Additionally, we evaluated the protective effect of PyoFun against non-ionizing radiation (monochromatic UV-C) and compared its efficacy to a synthetically produced control pyomelanin (PyoSyn). The results confirmed successful production of PyoFun in A. niger through inducible overproduction. Characterization using spectroscopic methods confirmed the presence of PyoFun, and the DPPH-assay demonstrated its strong antioxidant properties. Moreover, PyoFun exhibited a highly protective effect against radiation-induced stress, surpassing the protection provided by PyoSyn. The findings of this study suggest that PyoFun has significant potential as a biological shield against harmful radiation. Notably, PyoFun is synthesized extracellularly, differing it from other fungal melanins (such as L-DOPA- or DHN-melanin) that require cell lysis for pigment purification. This characteristic makes PyoFun a valuable resource for biotechnology, biomedicine, and the space industry. However, further research is needed to evaluate its protective effect in a dried form and against ionizing radiation.
590    __
$a NEINDEXOVÁNO
655    _2
$a časopisecké články $7 D016428
700    1_
$a Freidank-Pohl, Carsten $u Applied and Molecular Microbiology, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany
700    1_
$a Siontas, Oliver $u Applied and Molecular Microbiology, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany
700    1_
$a Cortesao, Marta $u Radiation Biology Department, Aerospace Microbiology Research Group, German Aerospace Center, Institute of Aerospace Medicine, Cologne, Germany
700    1_
$a Mota, Afonso $u Radiation Biology Department, Aerospace Microbiology Research Group, German Aerospace Center, Institute of Aerospace Medicine, Cologne, Germany
700    1_
$a Runzheimer, Katharina $u Radiation Biology Department, Aerospace Microbiology Research Group, German Aerospace Center, Institute of Aerospace Medicine, Cologne, Germany
700    1_
$a Jung, Sascha $u Applied and Molecular Microbiology, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany
700    1_
$a Rebrosova, Katarina $u Department of Microbiology, Faculty of Medicine, Masaryk University (MUNI) and St. Anne's Faculty Hospital, Brno, Czechia
700    1_
$a Siler, Martin $u Institute of Scientific Instruments of the Czech Academy of Sciences, Brno, Czechia
700    1_
$a Moeller, Ralf $u Radiation Biology Department, Aerospace Microbiology Research Group, German Aerospace Center, Institute of Aerospace Medicine, Cologne, Germany
700    1_
$a Meyer, Vera $u Applied and Molecular Microbiology, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany
773    0_
$w MED00181714 $t Frontiers in microbiology $x 1664-302X $g Roč. 14, č. - (2023), s. 1233740
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37547691 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20231010 $b ABA008
991    __
$a 20231020093503 $b ABA008
999    __
$a ok $b bmc $g 1997222 $s 1202041
BAS    __
$a 3
BAS    __
$a PreBMC-PubMed-not-MEDLINE
BMC    __
$a 2023 $b 14 $c - $d 1233740 $e 20230720 $i 1664-302X $m Frontiers in microbiology $n Front Microbiol $x MED00181714
LZP    __
$a Pubmed-20231010

Najít záznam

Citační ukazatele

Možnosti archivace