• Je něco špatně v tomto záznamu ?

A metatranscriptomic evaluation of viruses in field-collected bed bugs

JM. Sheele, V. Peta, A. Miron, O. Balvin, D. Cain, S. Edelheit, T. McCormick, JE. Pietri

. 2023 ; 123 (1) : 4. [pub] 20231205

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc24000295

Cimex lectularius, known as the common bed bug, is a widespread hematophagous human ectoparasite and urban pest that is not known to be a vector of any human infectious disease agents. However, few studies in the era of molecular biology have profiled the microorganisms harbored by field populations of bed bugs. The objective of this study was to examine the viruses present in a large sampling of common bed bugs and related bat bugs (Cimex pipistrelle). RNA sequencing was undertaken on an international sampling of > 500 field-collected bugs, and multiple workflows were used to assemble contigs and query these against reference nucleotide databases to identify viral genomes. Shuangao bed bug virus 2, an uncharacterized rhabdovirus previously discovered in Cimex hemipterus from China, was found in several bed bug pools from the USA and Europe, as well as in C. pipistrelle, suggesting that this virus is common among bed bug populations. In addition, Shuangao bed bug virus 1 was detected in a bed bug pool from China, and sequences matching Enterobacteria phage P7 were found in all bed bug pools, indicating the ubiquitous presence of phage-derived elements in the genome of the bed bug or its enterobacterial symbiont. However, viral diversity was low in bed bugs in our study, as no other viral genomes were detected with significant coverage. These results provide evidence against frequent virus infection in bed bugs. Nonetheless, our investigation had several important limitations, and additional studies should be conducted to better understand the prevalence and composition of viruses in bed bugs. Most notably, our study largely focused on insects from urban areas in industrialized nations, thus likely missing infrequent virus infections and those that could occur in rural or tropical environments or developing nations.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24000295
003      
CZ-PrNML
005      
20250312124232.0
007      
ta
008      
240109s2023 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s00436-023-08049-1 $2 doi
035    __
$a (PubMed)38049683
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Sheele, Johnathan M $u Department of Emergency Medicine, University Hospitals Cleveland Medical Center & Case Western Reserve University, Cleveland, OH, USA. jsheele@gmail.com $u Department of Emergency Medicine, Mayo Clinic, Jacksonville, FL, 32224, USA. jsheele@gmail.com $1 https://orcid.org/0000000345038590
245    12
$a A metatranscriptomic evaluation of viruses in field-collected bed bugs / $c JM. Sheele, V. Peta, A. Miron, O. Balvin, D. Cain, S. Edelheit, T. McCormick, JE. Pietri
520    9_
$a Cimex lectularius, known as the common bed bug, is a widespread hematophagous human ectoparasite and urban pest that is not known to be a vector of any human infectious disease agents. However, few studies in the era of molecular biology have profiled the microorganisms harbored by field populations of bed bugs. The objective of this study was to examine the viruses present in a large sampling of common bed bugs and related bat bugs (Cimex pipistrelle). RNA sequencing was undertaken on an international sampling of > 500 field-collected bugs, and multiple workflows were used to assemble contigs and query these against reference nucleotide databases to identify viral genomes. Shuangao bed bug virus 2, an uncharacterized rhabdovirus previously discovered in Cimex hemipterus from China, was found in several bed bug pools from the USA and Europe, as well as in C. pipistrelle, suggesting that this virus is common among bed bug populations. In addition, Shuangao bed bug virus 1 was detected in a bed bug pool from China, and sequences matching Enterobacteria phage P7 were found in all bed bug pools, indicating the ubiquitous presence of phage-derived elements in the genome of the bed bug or its enterobacterial symbiont. However, viral diversity was low in bed bugs in our study, as no other viral genomes were detected with significant coverage. These results provide evidence against frequent virus infection in bed bugs. Nonetheless, our investigation had several important limitations, and additional studies should be conducted to better understand the prevalence and composition of viruses in bed bugs. Most notably, our study largely focused on insects from urban areas in industrialized nations, thus likely missing infrequent virus infections and those that could occur in rural or tropical environments or developing nations.
650    _2
$a zvířata $7 D000818
650    _2
$a lidé $7 D006801
650    12
$a štěnice $x genetika $7 D001511
650    12
$a infestace ektoparazity $7 D004478
650    12
$a viry $x genetika $7 D014780
651    _2
$a Evropa $7 D005060
651    _2
$a Čína $7 D002681
655    _2
$a časopisecké články $7 D016428
700    1_
$a Peta, Vincent $u Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD, 57069, USA
700    1_
$a Miron, Alexander $u Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, OH, USA
700    1_
$a Balvín, Ondřej $u Department of Ecology, Czech University of Life Sciences Prague, Prague, Czech Republic $7 xx0329898
700    1_
$a Cain, David $u Bed Bugs Limited, 3 Cobden Road, London, UK
700    1_
$a Edelheit, Simone $u Department of Dermatology, Case Western Reserve University, Cleveland, OH, USA
700    1_
$a McCormick, Tom $u Department of Dermatology, Case Western Reserve University, Cleveland, OH, USA
700    1_
$a Pietri, Jose E $u Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD, 57069, USA. Jose.Pietri@usd.edu
773    0_
$w MED00003691 $t Parasitology research $x 1432-1955 $g Roč. 123, č. 1 (2023), s. 4
856    41
$u https://pubmed.ncbi.nlm.nih.gov/38049683 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240109 $b ABA008
991    __
$a 20250312124239 $b ABA008
999    __
$a ok $b bmc $g 2049140 $s 1209989
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 123 $c 1 $d 4 $e 20231205 $i 1432-1955 $m Parasitology research $n Parasitol Res $x MED00003691
LZP    __
$a Pubmed-20240109

Najít záznam

Citační ukazatele

Možnosti archivace