-
Je něco špatně v tomto záznamu ?
Zhilong Huoxue Tongyu capsule alleviates myocardial fibrosis by improving endothelial cell dysfunction
T. Bi, Y. Zhou, L. Mao, P. Liang, J. Liu, L. Yang, G. Ren, M. Mazhar, H. Shen, P. Liu, R. Spáčil, Q. Guo, G. Luo, S. Yang, W. Ren
Status neindexováno Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
NLK
Directory of Open Access Journals
od 2015
Free Medical Journals
od 2011
PubMed Central
od 2011
Europe PubMed Central
od 2011
Open Access Digital Library
od 2011-01-01
Open Access Digital Library
od 2015-01-01
- Publikační typ
- časopisecké články MeSH
BACKGROUND AND AIM: Zhilong Huoxue Tongyu (ZL) capsule is a classical traditional Chinese medicine (TCM) with satisfactory curative effects. Endothelial cell (EC) dysfunction plays an important role during myocardial fibrosis (MF). But the therapeutic effect of ZL capsule on EC dysfunction remains unknown in the development of MF. This study aims to investigate the effect of ZL capsule on EC dysfunction during MF in vivo. EXPERIMENTAL PROCEDURE: The model of MF is established in vivo by injecting isoproterenol for 14 days, simultaneously, we examined the therapeutic effect of ZL capsule on MF in vivo. An integrative approach combining biomarker examination, echocardiography and myocardial fibrosis condition using Hematoxylin-eosin staining, Masson staining, and Sirius red staining were performed to assess the efficacy of ZL capsule against MF. Subsequently, comprehensive immunofluorescence staining was performed to evaluate the therapeutic effect of ZL capsule on EC dysfunction. RESULTS AND CONCLUSION: Prior to experiments, analysis of the published single-cell sequencing data was performed and it was discovered that EC dysfunction plays an important role. Further pharmacological results showed that ZL capsule could alleviate fibrosis injury and collagen fiber deposition. The mechanism investigation results showed that the endothelial-to-mesenchymal transition (EndMT) and MHC class-II (MHC-II) expression in EC were improved. In addition, ZL capsule can attenuate the inflammatory response during MF by intervening the activation of CD4+T cell mediated by EC. For the first time, we provided evidence that ZL capsule could improve MF by alleviating EC dysfunction via the regulation of EndMT and expression of MHC-II. TAXONOMY CLASSIFICATION BY EVISE: Myocardial fibrosis, Chinese Herbal Medicine, Traditional Medicine, Endothelium, dysfunction, Endothelial-to-mesenchymal transition.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc24006108
- 003
- CZ-PrNML
- 005
- 20240412130931.0
- 007
- ta
- 008
- 240405s2024 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.jtcme.2023.07.001 $2 doi
- 035 __
- $a (PubMed)38223805
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Bi, Tao $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China
- 245 10
- $a Zhilong Huoxue Tongyu capsule alleviates myocardial fibrosis by improving endothelial cell dysfunction / $c T. Bi, Y. Zhou, L. Mao, P. Liang, J. Liu, L. Yang, G. Ren, M. Mazhar, H. Shen, P. Liu, R. Spáčil, Q. Guo, G. Luo, S. Yang, W. Ren
- 520 9_
- $a BACKGROUND AND AIM: Zhilong Huoxue Tongyu (ZL) capsule is a classical traditional Chinese medicine (TCM) with satisfactory curative effects. Endothelial cell (EC) dysfunction plays an important role during myocardial fibrosis (MF). But the therapeutic effect of ZL capsule on EC dysfunction remains unknown in the development of MF. This study aims to investigate the effect of ZL capsule on EC dysfunction during MF in vivo. EXPERIMENTAL PROCEDURE: The model of MF is established in vivo by injecting isoproterenol for 14 days, simultaneously, we examined the therapeutic effect of ZL capsule on MF in vivo. An integrative approach combining biomarker examination, echocardiography and myocardial fibrosis condition using Hematoxylin-eosin staining, Masson staining, and Sirius red staining were performed to assess the efficacy of ZL capsule against MF. Subsequently, comprehensive immunofluorescence staining was performed to evaluate the therapeutic effect of ZL capsule on EC dysfunction. RESULTS AND CONCLUSION: Prior to experiments, analysis of the published single-cell sequencing data was performed and it was discovered that EC dysfunction plays an important role. Further pharmacological results showed that ZL capsule could alleviate fibrosis injury and collagen fiber deposition. The mechanism investigation results showed that the endothelial-to-mesenchymal transition (EndMT) and MHC class-II (MHC-II) expression in EC were improved. In addition, ZL capsule can attenuate the inflammatory response during MF by intervening the activation of CD4+T cell mediated by EC. For the first time, we provided evidence that ZL capsule could improve MF by alleviating EC dysfunction via the regulation of EndMT and expression of MHC-II. TAXONOMY CLASSIFICATION BY EVISE: Myocardial fibrosis, Chinese Herbal Medicine, Traditional Medicine, Endothelium, dysfunction, Endothelial-to-mesenchymal transition.
- 590 __
- $a NEINDEXOVÁNO
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Zhou, Yanan $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China
- 700 1_
- $a Mao, Linshen $u Department of Cardiovascular Medicine, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan, China
- 700 1_
- $a Liang, Pan $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China $u State Key Laboratories for Quality Research in Chinese Medicines, Faculty of Chinese Medicine, Macau University of Science and Technology, Macau, 853, China
- 700 1_
- $a Liu, Jiali $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China
- 700 1_
- $a Yang, Luyin $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China
- 700 1_
- $a Ren, Guilin $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China
- 700 1_
- $a Mazhar, Maryam $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u The National T.C.M Service Export Base of the Affiliated T.C.M Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China
- 700 1_
- $a Shen, Hongping $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China
- 700 1_
- $a Liu, Ping $u Department of Cardiovascular Medicine, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan, China
- 700 1_
- $a Spáčil, Roman $u The Czech Center for Traditional Chinese Medicine, Jeremenkova 1211/40, Olomouc, 77900, Czech Republic
- 700 1_
- $a Guo, Qing $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China
- 700 1_
- $a Luo, Gang $u Department of Cardiovascular Medicine, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan, China
- 700 1_
- $a Yang, Sijin $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u The National T.C.M Service Export Base of the Affiliated T.C.M Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China $u State Key Laboratories for Quality Research in Chinese Medicines, Faculty of Chinese Medicine, Macau University of Science and Technology, Macau, 853, China
- 700 1_
- $a Ren, Wei $u National Traditional Chinese Medicine Clinical Research Base and Drug Research Center of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000, China $u The National T.C.M Service Export Base of the Affiliated T.C.M Hospital of Southwest Medical University, Luzhou, 646000, China $u Institute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000, China
- 773 0_
- $w MED00214675 $t Journal of traditional and complementary medicine $x 2225-4110 $g Roč. 14, č. 1 (2024), s. 40-54
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/38223805 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20240405 $b ABA008
- 991 __
- $a 20240412130924 $b ABA008
- 999 __
- $a ok $b bmc $g 2076129 $s 1215870
- BAS __
- $a 3
- BAS __
- $a PreBMC-PubMed-not-MEDLINE
- BMC __
- $a 2024 $b 14 $c 1 $d 40-54 $e 20230712 $i 2225-4110 $m Journal of traditional and complementary medicine $n J Tradit Complement Med $x MED00214675
- LZP __
- $a Pubmed-20240405
