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Impact of media supplements FGF2, LIF and IGF1 on the genome activity of porcine embryos produced in vitro

A. Rosenbaum Bartkova, L. Nemcova, F. Strejcek, A. Gad, V. Kinterova, M. Morovic, M. Benc, R. Prochazka, J. Laurincik

. 2024 ; 14 (1) : 7081. [pub] 20240325

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc24006664

Grantová podpora
DS-FR-22-0003 Agentúra na Podporu Výskumu a Vývoja
CZ.02.1.01/0.0/0.0/15_003/0000460 Ministerstvo Školství, Mládeže a Tělovýchovy
VEGA 1/0167/20 Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
039UKF-4/2021 Kultúrna a Edukacná Grantová Agentúra MŠVVaŠ SR
23-05108S Grantová Agentura České Republiky

In this article, we focused on the impact of precisely chemically modified FLI maturation medium enriched with fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), insulin-like growth factor 1 (IGF1), and polyvinyl alcohol (PVA) and its potential to improve the efficiency of in vitro production of porcine embryos. We hypothesized that enhancing the composition of the maturation medium could result in an elevated production of embryos in vitro and can affect EGA. FLI medium resulted in a significantly higher rate of oocyte blastocyst maturation and formation compared to the control DMEM medium. In addition, immunocytochemical labelling confirmed the detection of UBF in 4-cell FLI parthenogenic embryos, suggesting similarities with natural embryo development. Through RNAseq analysis, upregulated genes present in 4-cell FLI embryos were found to play key roles in important biological processes such as cell proliferation, cell differentiation, and transcriptional regulation. Based on our findings, we demonstrated the positive influence of FLI medium in the evaluation of in vitro embryo production, EGA detection, transcriptomic and proteomic profile, which was confirmed by the positive activation of the embryonal genome in the 4-cell stage of parthenogenetically activated embryos.

Citace poskytuje Crossref.org

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