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In silico prediction of the metabolism of Blastocrithidia nonstop, a trypanosomatid with non-canonical genetic code

FR. Opperdoes, K. Záhonová, I. Škodová-Sveráková, B. Bučková, Ľ. Chmelová, J. Lukeš, V. Yurchenko

. 2024 ; 25 (1) : 184. [pub] 20240216

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc24007107

Grantová podpora
CZ.02.1.01/16_019/ 0000759 European Regional Development Fund
CZ.02.1.01/16_019/ 0000759 European Regional Development Fund
CZ.02.1.01/16_019/ 0000759 European Regional Development Fund
SK-CZ-RD-21-0038 Agentúra na Podporu Výskumu a Vývoja
SK-CZ-RD-21-0038 Agentúra na Podporu Výskumu a Vývoja
SGS/PřF/2023 Ostravská Univerzita v Ostravě
SGS/PřF/2023 Ostravská Univerzita v Ostravě
22-14356S Grantová Agentura České Republiky
22-14356S Grantová Agentura České Republiky
22-14356S Grantová Agentura České Republiky
CZ.10.03.01/00/22_003/0000003 European Union's Operational Program LERCO
CZ.10.03.01/00/22_003/0000003 European Union's Operational Program LERCO
e-INFRA CZ 90254 Ministerstvo Školství, Mládeže a Tělovýchovy
LUASK22033 Ministerstvo školství, mládeže a tělovýchovy České republiky

BACKGROUND: Almost all extant organisms use the same, so-called canonical, genetic code with departures from it being very rare. Even more exceptional are the instances when a eukaryote with non-canonical code can be easily cultivated and has its whole genome and transcriptome sequenced. This is the case of Blastocrithidia nonstop, a trypanosomatid flagellate that reassigned all three stop codons to encode amino acids. RESULTS: We in silico predicted the metabolism of B. nonstop and compared it with that of the well-studied human parasites Trypanosoma brucei and Leishmania major. The mapped mitochondrial, glycosomal and cytosolic metabolism contains all typical features of these diverse and important parasites. We also provided experimental validation for some of the predicted observations, concerning, specifically presence of glycosomes, cellular respiration, and assembly of the respiratory complexes. CONCLUSIONS: In an unusual comparison of metabolism between a parasitic protist with a massively altered genetic code and its close relatives that rely on a canonical code we showed that the dramatic differences on the level of nucleic acids do not seem to be reflected in the metabolisms. Moreover, although the genome of B. nonstop is extremely AT-rich, we could not find any alterations of its pyrimidine synthesis pathway when compared to other trypanosomatids. Hence, we conclude that the dramatic alteration of the genetic code of B. nonstop has no significant repercussions on the metabolism of this flagellate.

Citace poskytuje Crossref.org

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