The skin is the primary tissue affected by wounds and aging, significantly impacting its protective function. Natural products are widely used in cosmetics, representing a new approach to preventing age-related damage. Nanomedicine combines nanotechnology and traditional treatments to create innovative drugs. The main targets of nanotechnological approaches are wound healing, regeneration, and rejuvenation of skin tissue. The skin barrier is not easily permeable, and the creation of modern nanodevices is a way to improve the passive penetration of substances. In this study, Helichrysum italicum oil (HO) was combined with different types of electrospun nanofibers to study their protective activity on the skin and to evaluate their future application for topical treatments. In the present research, we used biodegradable polymers, including polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP), which were characterized by a scanning electron microscope (SEM). All results show a positive trend in cell proliferation and viability of human skin stem cells (SSCs) and BJ fibroblasts pre-treated with combined nanofibers and then exposed to UV stress. Gene expression analysis revealed the activation of a molecular rejuvenation program in SSCs treated with functionalized nanofibers before UV exposure. Understanding the mechanisms involved in skin changes during aging allows for the future application of nanomaterials combined with HO directly to the patients.

Center for Developmental Biology and Reprogramming CEDEBIOR Department of Biomedical Sciences University of Sassari Viale San Pietro 43 B 07100 Sassari Italy

Department of Biomedical Sciences University of Sassari Viale San Pietro 43 B 07100 Sassari Italy

Department of Biophysics 2nd Faculty of Medicine Charles University 5 Uvalu 84 150 06 Prague Czech Republic

Department of Medical Surgical and Experimental Sciences University of Sassari 07100 Sassari Italy

Department of Medicine Surgery and Pharmacy University of Sassari 07100 Sassari Italy

Fraunhofer Institute for Biomedical Engineering IBMT Joseph von Fraunhofer Weg 1 66280 Sulzbach Germany

Laboratory of Molecular Biology and Stem Cell Engineering National Institute of Biostructures and Biosystems Eldor Lab Innovation Accelerator CNR Via Piero Gobetti 101 40129 Bologna Italy

R and D Laboratory Center InoCure s r o Politických Veziu 935 13 110 00 Prague Czech Republic

University Centre for Energy Efficient Buildings Czech Technical University Prague Trinecka 1024 273 43 Bustehrad Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc24007199

003      

CZ-PrNML

005      

20240423155802.0

007      

ta

008      

240412s2024 sz f 000 0|eng||

009      

AR

024    7_

$a 10.3390/ijms25031908 $2 doi

035    __

$a (PubMed)38339184

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a sz

100    1_

$a Serra, Diletta $u Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy $u R&D Laboratory Center, InoCure s.r.o., Politických Veziu 935/13, 110 00 Prague, Czech Republic

245    10

$a Electrospun Nanofibers Encapsulated with Natural Products: A Novel Strategy to Counteract Skin Aging / $c D. Serra, G. Garroni, S. Cruciani, D. Coradduzza, A. Pashchenko, E. Amler, G. Pintore, R. Satta, MA. Montesu, Y. Kohl, C. Ventura, M. Maioli

520    9_

$a The skin is the primary tissue affected by wounds and aging, significantly impacting its protective function. Natural products are widely used in cosmetics, representing a new approach to preventing age-related damage. Nanomedicine combines nanotechnology and traditional treatments to create innovative drugs. The main targets of nanotechnological approaches are wound healing, regeneration, and rejuvenation of skin tissue. The skin barrier is not easily permeable, and the creation of modern nanodevices is a way to improve the passive penetration of substances. In this study, Helichrysum italicum oil (HO) was combined with different types of electrospun nanofibers to study their protective activity on the skin and to evaluate their future application for topical treatments. In the present research, we used biodegradable polymers, including polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP), which were characterized by a scanning electron microscope (SEM). All results show a positive trend in cell proliferation and viability of human skin stem cells (SSCs) and BJ fibroblasts pre-treated with combined nanofibers and then exposed to UV stress. Gene expression analysis revealed the activation of a molecular rejuvenation program in SSCs treated with functionalized nanofibers before UV exposure. Understanding the mechanisms involved in skin changes during aging allows for the future application of nanomaterials combined with HO directly to the patients.

650    _2

$a lidé $7 D006801

650    12

$a nanovlákna $7 D057139

650    12

$a stárnutí kůže $7 D015595

650    12

$a biologické přípravky $x farmakologie $7 D001688

650    _2

$a kůže $7 D012867

650    _2

$a hojení ran $7 D014945

650    _2

$a polyvinylalkohol $7 D011142

655    _2

$a časopisecké články $7 D016428

700    1_

$a Garroni, Giuseppe $u Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy $1 https://orcid.org/0000000203892142

700    1_

$a Cruciani, Sara $u Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy $1 https://orcid.org/0000000186322577

700    1_

$a Coradduzza, Donatella $u Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy

700    1_

$a Pashchenko, Aleksei $u Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy $u Department of Biophysics, Second Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic $u University Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 273 43 Bustehrad, Czech Republic $1 https://orcid.org/0000000313153770

700    1_

$a Amler, Evzen $u University Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 273 43 Bustehrad, Czech Republic

700    1_

$a Pintore, Giorgio $u Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100 Sassari, Italy

700    1_

$a Satta, Rosanna $u Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy

700    1_

$a Montesu, Maria Antonietta $u Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy

700    1_

$a Kohl, Yvonne $u Fraunhofer Institute for Biomedical Engineering IBMT, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany $1 https://orcid.org/0000000181195441

700    1_

$a Ventura, Carlo $u Laboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems-Eldor Lab, Innovation Accelerator, CNR, Via Piero Gobetti 101, 40129 Bologna, Italy $1 https://orcid.org/0000000193330321

700    1_

$a Maioli, Margherita $u Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy $u Center for Developmental Biology and Reprogramming-CEDEBIOR, Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy $1 https://orcid.org/0000000301874968 $7 xx0271022

773    0_

$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 25, č. 3 (2024)

856    41

$u https://pubmed.ncbi.nlm.nih.gov/38339184 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y - $z 0

990    __

$a 20240412 $b ABA008

991    __

$a 20240423155759 $b ABA008

999    __

$a ok $b bmc $g 2081280 $s 1216966

BAS    __

$a 3

BAS    __

$a PreBMC-MEDLINE

BMC    __

$a 2024 $b 25 $c 3 $e 20240205 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142

LZP    __

$a Pubmed-20240412