• Je něco špatně v tomto záznamu ?

Modified live vaccine strains of porcine reproductive and respiratory syndrome virus cause immune system dysregulation similar to wild strains

K. Stepanova, M. Toman, J. Sinkorova, S. Sinkora, S. Pfeiferova, H. Kupcova Skalnikova, S. Abuhajiar, R. Moutelikova, J. Salat, H. Stepanova, K. Nechvatalova, L. Leva, P. Hermanova, M. Kratochvilova, B. Dusankova, M. Sinkora, V. Horak, T....

. 2023 ; 14 (-) : 1292381. [pub] 20240112

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc24007584

INTRODUCTION: Porcine reproductive and respiratory syndrome virus (PRRSV) emerged about 30 years ago and continues to cause major economic losses in the pork industry. The lack of effective modified live vaccines (MLV) allows the pandemic to continue. BACKGROUND AND OBJECTIVE: We have previously shown that wild strains of PRRSV affect the nascent T cell repertoire in the thymus, deplete T cell clones recognizing viral epitopes essential for neutralization, while triggering a chronic, robust, but ineffective antibody response. Therefore, we hypothesized that the current MLV are inappropriate because they cause similar damage and fail to prevent viral-induced dysregulation of adaptive immunity. METHODS: We tested three MLV strains to demonstrate that all have a comparable negative effect on thymocytes in vitro. Further in vivo studies compared the development of T cells in the thymus, peripheral lymphocytes, and antibody production in young piglets. These three MLV strains were used in a mixture to determine whether at least some of them behave similarly to the wild virus type 1 or type 2. RESULTS: Both the wild and MLV strains cause the same immune dysregulations. These include depletion of T-cell precursors, alteration of the TCR repertoire, necrobiosis at corticomedullary junctions, low body weight gain, decreased thymic cellularity, lack of virus-neutralizing antibodies, and production of non-neutralizing anti-PRRSV antibodies of different isotypes. DISCUSSION AND CONCLUSION: The results may explain why the use of current MLV in young animals may be ineffective and why their use may be potentially dangerous. Therefore, alternative vaccines, such as subunit or mRNA vaccines or improved MLV, are needed to control the PRRSV pandemic.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24007584
003      
CZ-PrNML
005      
20240423160107.0
007      
ta
008      
240412e20240112sz f 000 0|eng||
009      
AR
024    7_
$a 10.3389/fimmu.2023.1292381 $2 doi
035    __
$a (PubMed)38283357
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Stepanova, Katerina $u Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia
245    10
$a Modified live vaccine strains of porcine reproductive and respiratory syndrome virus cause immune system dysregulation similar to wild strains / $c K. Stepanova, M. Toman, J. Sinkorova, S. Sinkora, S. Pfeiferova, H. Kupcova Skalnikova, S. Abuhajiar, R. Moutelikova, J. Salat, H. Stepanova, K. Nechvatalova, L. Leva, P. Hermanova, M. Kratochvilova, B. Dusankova, M. Sinkora, V. Horak, T. Hudcovic, JE. Butler, M. Sinkora
520    9_
$a INTRODUCTION: Porcine reproductive and respiratory syndrome virus (PRRSV) emerged about 30 years ago and continues to cause major economic losses in the pork industry. The lack of effective modified live vaccines (MLV) allows the pandemic to continue. BACKGROUND AND OBJECTIVE: We have previously shown that wild strains of PRRSV affect the nascent T cell repertoire in the thymus, deplete T cell clones recognizing viral epitopes essential for neutralization, while triggering a chronic, robust, but ineffective antibody response. Therefore, we hypothesized that the current MLV are inappropriate because they cause similar damage and fail to prevent viral-induced dysregulation of adaptive immunity. METHODS: We tested three MLV strains to demonstrate that all have a comparable negative effect on thymocytes in vitro. Further in vivo studies compared the development of T cells in the thymus, peripheral lymphocytes, and antibody production in young piglets. These three MLV strains were used in a mixture to determine whether at least some of them behave similarly to the wild virus type 1 or type 2. RESULTS: Both the wild and MLV strains cause the same immune dysregulations. These include depletion of T-cell precursors, alteration of the TCR repertoire, necrobiosis at corticomedullary junctions, low body weight gain, decreased thymic cellularity, lack of virus-neutralizing antibodies, and production of non-neutralizing anti-PRRSV antibodies of different isotypes. DISCUSSION AND CONCLUSION: The results may explain why the use of current MLV in young animals may be ineffective and why their use may be potentially dangerous. Therefore, alternative vaccines, such as subunit or mRNA vaccines or improved MLV, are needed to control the PRRSV pandemic.
650    _2
$a zvířata $7 D000818
650    _2
$a prasata $7 D013552
650    12
$a virus reprodukčního a respiračního syndromu prasat $7 D019316
650    12
$a reprodukční a respirační syndrom prasat $x prevence a kontrola $7 D019318
650    _2
$a protilátky virové $7 D000914
650    _2
$a atenuované vakcíny $7 D014613
650    _2
$a imunitní systém $7 D007107
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Toman, Miroslav $u Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czechia
700    1_
$a Sinkorova, Jana $u Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia
700    1_
$a Sinkora, Simon $u Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia
700    1_
$a Pfeiferova, Sarka $u Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia
700    1_
$a Kupcova Skalnikova, Helena $u Laboratory of Applied Proteome Analyses and Research Center PIGMOD (Pig Models of Diseases), Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czechia $u Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czechia
700    1_
$a Abuhajiar, Salim $u Laboratory of Applied Proteome Analyses and Research Center PIGMOD (Pig Models of Diseases), Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czechia $u Department of Cell Biology, Faculty of Science, Charles University, Prague, Czechia
700    1_
$a Moutelikova, Romana $u Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czechia
700    1_
$a Salat, Jiri $u Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czechia
700    1_
$a Stepanova, Hana $u Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czechia
700    1_
$a Nechvatalova, Katerina $u Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czechia
700    1_
$a Leva, Lenka $u Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czechia
700    1_
$a Hermanova, Petra $u Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia
700    1_
$a Kratochvilova, Mirka $u Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia
700    1_
$a Dusankova, Blanka $u Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia
700    1_
$a Sinkora, Marek $u Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia
700    1_
$a Horak, Vratislav $u Laboratory of Applied Proteome Analyses and Research Center PIGMOD (Pig Models of Diseases), Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czechia
700    1_
$a Hudcovic, Tomas $u Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia
700    1_
$a Butler, John E $u Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States
700    1_
$a Sinkora, Marek $u Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia
773    0_
$w MED00181405 $t Frontiers in immunology $x 1664-3224 $g Roč. 14 (20240112), s. 1292381
856    41
$u https://pubmed.ncbi.nlm.nih.gov/38283357 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240412 $b ABA008
991    __
$a 20240423160104 $b ABA008
999    __
$a ok $b bmc $g 2081527 $s 1217351
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 14 $c - $d 1292381 $e 20240112 $i 1664-3224 $m Frontiers in immunology $n Front Immunol $x MED00181405
LZP    __
$a Pubmed-20240412

Najít záznam

Citační ukazatele

Možnosti archivace