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The killifish germline regulates longevity and somatic repair in a sex-specific manner
E. Moses, T. Atlan, X. Sun, R. Franěk, A. Siddiqui, GK. Marinov, S. Shifman, DM. Zucker, A. Oron-Gottesman, WJ. Greenleaf, E. Cohen, O. Ram, I. Harel
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
StG #101078188
EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
19/HU04
Abisch-Frenkel-Stiftung (Abisch-Frenkel Foundation)
2178/19
Israel Science Foundation (ISF)
GBMF9341
Gordon and Betty Moore Foundation (Gordon E. and Betty I. Moore Foundation)
2020611
United States - Israel Binational Science Foundation (BSF)
#CZ.02.1.01/0.0/0.0/16_025/0007370
Ministerstvo Školství, Mládeže a Tělovýchovy (Ministry of Education, Youth and Sports)
P50HG007735
U.S. Department of Health & Human Services | National Institutes of Health (NIH)
UM1HG009442
U.S. Department of Health & Human Services | National Institutes of Health (NIH)
1UM1HG009436
U.S. Department of Health & Human Services | National Institutes of Health (NIH)
NLK
ProQuest Central
od 2021-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2021-01-01 do Před 1 rokem
Public Health Database (ProQuest)
od 2021-01-01 do Před 1 rokem
- MeSH
- Caenorhabditis elegans genetika fyziologie MeSH
- dlouhověkost * genetika MeSH
- pohlavní dimorfismus MeSH
- zárodečné buňky * metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Classical evolutionary theories propose tradeoffs among reproduction, damage repair and lifespan. However, the specific role of the germline in shaping vertebrate aging remains largely unknown. In this study, we used the turquoise killifish (Nothobranchius furzeri) to genetically arrest germline development at discrete stages and examine how different modes of infertility impact life history. We first constructed a comprehensive single-cell gonadal atlas, providing cell-type-specific markers for downstream phenotypic analysis. We show here that germline depletion-but not arresting germline differentiation-enhances damage repair in female killifish. Conversely, germline-depleted males instead showed an extension in lifespan and rejuvenated metabolic functions. Through further transcriptomic analysis, we highlight enrichment of pro-longevity pathways and genes in germline-depleted male killifish and demonstrate functional conservation of how these factors may regulate longevity in germline-depleted Caenorhabditis elegans. Our results, therefore, demonstrate that different germline manipulation paradigms can yield pronounced sexually dimorphic phenotypes, implying alternative responses to classical evolutionary tradeoffs.
Center for Personal Dynamic Regulomes Stanford University Stanford CA USA
Chan Zuckerberg Biohub San Francisco CA USA
Department of Applied Physics Stanford University Stanford CA USA
Department of Genetics Silberman Institute Hebrew University of Jerusalem Givat Ram Jerusalem Israel
Department of Genetics Stanford University Stanford CA USA
Department of Statistics and Data Science Hebrew University of Jerusalem Jerusalem Israel
Citace poskytuje Crossref.org
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